| Long name: |
BRCA2 Primary Polyclonal Antibody |
| Also known as: |
BRCA2 PAb |
| Category: |
Primary Antibodies |
| Conjugation: |
Unconjugated |
| Target Antigen: |
BRCA2 |
| Specificity: |
This is a highly specific antibody against BRCA2 |
| Modification(s): |
None |
| Modification site(s): |
Unmodified antibody |
| Clonality: |
Polyclonal antibody |
| Clone number: |
Polyclonal antibody |
| Concentration: |
1ug per 1ul |
| Immunogen range: |
1-50/3418 |
| Subcellular location: |
Nucleus, Cytoplasm |
| Source: |
This antibody was obtained by immunization of the host with KLH conjugated synthetic peptide derived from human BRCA2 |
| Gene ID number: |
675 |
| Swiss Prot: |
P51587 |
| Tested Applications: |
IF(IHC-P), IHC-P, WB |
| Recommended dilutions: |
IF(IHC-P)(1:50-200), IHC-P(1:100-500), WB(1:100-1000) |
| Cross reactivity: |
Mouse, Rat, Human |
| Cross reactive species details: |
not every possible cross-reactivity is known, Due to limited amount of testing and knowledge |
| Background of the target antigen: |
Acts by targeting RAD51 to ssDNA over double-stranded DNA, Binds RAD51 and potentiates recombinational DNA repair by promoting assembly of RAD51 onto single-stranded DNA (ssDNA), Binds selectively to ssDNA, In concert with NPM1, Interacts with the TREX-2 complex (transcription and export complex 2) subunits PCID2 and DSS1, May participate in S phase checkpoint activation, May play a role in the extension step after strand invasion at replication-dependent DNA double-strand breaks, Part of a PALB2-scaffolded HR complex containing RAD51C and which is thought to play a role in DNA repair by HR, Silencing of BRCA2 promotes R-loop accumulation at actively transcribed genes in replicating and non-replicating cells, and is required to prevent R-loop-associated DNA damage and thus transcription-associated genomic instability, and to ssDNA in tailed duplexes and replication fork structures, enabling RAD51 to displace replication protein-A (RPA) from ssDNA and stabilizing RAD51-ssDNA filaments by blocking ATP hydrolysis, independently of its known role in homologous recombination (PubMed:24896180), regulates centrosome duplication, suggesting that BRCA2 mediates the control of R-loop associated genomic instability, together with PALB2 is involved in both POLH localization at collapsed replication forks and DNA polymerization activity, Involved in double-strand break repair and/or homologous recombination |
| Purification method: |
This antibody was purified via Protein A |
| Storage conditions: |
50% glycerol and 0, Store at -20°, 09% sodium azide, C for up to 1 year, Keep the antibody in aqueous buffered solution containing 1% BSA |
| Synonym names: |
BRCA2, BRCC2, BROVCA2, Breast cancer type 2 susceptibility protein, FACD, FAD1, FANCD, FANCD1, Fanconi anemia group D1 protein, GLM3, PNCA2, FAD |
| Properties: |
C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Unconjugated Antibodies they should be stored frozen at - 24° |
| Gene target: |
BRCA2 |
| Short name: |
BRCA2 Antibody |
| Technique: |
antibodies against human proteins, antibodies for, Antibody |
| Alternative name: |
early onset (Antibody to), breast cancer 2 |
| Alternative technique: |
antibodies |
| Alternative to gene target: |
BRCA2 and IDBG-22703 and ENSG00000139618 and 675, BRCA2 and IDBG-630223 and ENSBTAG00000000988 and, BRCC2 and BROVCA2 and FACD and FAD and FAD1 and FANCD and FANCD1 and GLM3 and PNCA2, Brca2 and IDBG-209587 and ENSMUSG00000041147 and 12190, DNA-templated and biological process this GO :0045931 and positive regulation of mitotic cell cycle and biological process this GO :0048478 and replication fork protection and biological process this GO :0051276 and chromosome organization and biological process this GO :0051298 and centrosome duplication and biological process, early onset, gamma-tubulin binding, nuclei, signal transduction by p53 class mediator resulting in transcription of p21 class mediator and biological process this GO :0007141 and male meiosis I and biological process this GO :0007283 and spermatogenesis and biological process this GO :0007420 and brain development and biological process this GO :0007569 and cell aging and biological process this GO :0008283 and cell proliferation and biological process this GO :0008585 and female this GO nad development and biological process this GO :0008630 and intrinsic apoptotic signaling pathway in response to DNA damage and biological process this GO :0010165 and response to X-ray and biological process this GO :0010225 and response to UV-C and biological process this GO :0010332 and response to gamma radiation and biological process this GO :0010484 and H3 histone acetyltransferase activity and molecular function this GO :0010485 and H4 histone acetyltransferase activity and molecular function this GO :0030097 and hemopoiesis and biological process this GO :0030141 and secretory granule and cellular component this GO :0032465 and regulation of cytokinesis and biological process this GO :0033593 and BRCA2-MAGE-D1 complex and cellular component this GO :0033600 and negative regulation of mammary gland epithelial cell proliferation and biological process this GO :0042771 and intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator and biological process this GO :0043009 and chordate embryonic development and biological process this GO :0043015 and gamma-tubulin binding and molecular function this GO :0043234 and protein complex and cellular component this GO :0043966 and histone H3 acetylation and biological process this GO :0043967 and histone H4 acetylation and biological process this GO :0045893 and positive regulation of transcription, this GO :0000724 and double-strand break repair via homolo this GO us recombination and biological process this GO :0000910 and cytokinesis and biological process this GO :0001556 and oocyte maturation and biological process this GO :0001833 and inner cell mass cell proliferation and biological process this GO :0002020 and protease binding and molecular function this GO :0003697 and single-stranded DNA binding and molecular function this GO :0005515 and protein binding and molecular function this GO :0005634 and nucleus and cellular component this GO :0005654 and nucleoplasm and cellular component this GO :0005737 and cytoplasm and cellular component this GO :0005813 and centrosome and cellular component this GO :0006281 and DNA repair and biological process this GO :0006289 and nucleotide-excision repair and biological process this GO :0006302 and double-strand break repair and biological process this GO :0006974 and cellular response to DNA damage stimulus and biological process this GO :0006978 and DNA damage response, this GO :0002020 : protease binding, this GO :0002020 : protease binding and also this GO :0003697 : single-stranded DNA binding and also this GO :0005515 : protein binding and also this GO :0010484 : H3 histone acetyltransferase activity and also this GO :0010485 : H4 histone acetyltransferase activity and also this GO :0043015 : gamma-tubulin binding, this GO :0003697 : single-stranded DNA binding, this GO :0005515 : protein binding, this GO :0010484 : H3 histone acetyltransferase activity, this GO :0010485 : H4 histone acetyltransferase activity, this GO :0043015 : gamma-tubulin binding, breast cancer 2 |
| Identity: |
1101 |
| Gene: |
BRCA2 |
More about : BRCA2 |
| Long gene name: |
BRCA2, DNA repair associated |
| Synonyms gene: |
FANCD1 FACD FANCD |
| Synonyms gene name: |
Fanconi anemia, complementation group D1 breast cancer 2, early onset breast cancer 2 |
| Synonyms: |
FAD FAD1 BRCC2 XRCC11 |
| Synonyms name: |
BRCA1/BRCA2-containing complex, subunit 2 |
| Locus: |
13q13, 1 |
| Discovery year: |
1994-10-17 |
| GenBank acession: |
U43746 |
| Entrez gene record: |
675 |
| Pubmed identfication: |
8091231 7581463 15057823 |
| RefSeq identity: |
NM_000059 |
| Classification: |
Fanconi anemia complementation groups |
| Havana BLAST/BLAT: |
OTTHUMG00000017411 |
| Locus Specific Databases: |
Breast Cancer Fanconi Anaemia Mutation Database BRCA1 database at LOVD-China LOVD - Leiden Open Variation Database LOVD - Leiden Open Variation Database Fanconi anemia database LRG_293 |