| Long name: |
FITC Conjugated, RhoA/C Polyclonal Antibody |
| Category: |
Conjugated Primary Antibodies |
| Conjugation: |
Anti-FITC Antibody |
| Host Organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Antigen: |
RhoA/C |
| Specificity: |
This is a highly specific antibody against RhoA/C |
| Modification: |
Unmodified |
| Modification site: |
None |
| Clonality: |
Polyclonal |
| Clone: |
Polyclonal antibody |
| Concentration: |
1ug per 1ul |
| Immunogen_range: |
90-140/193 |
| Subcellular location: |
Helical, Cytoplasm |
| Source: |
KLH conjugated synthetic peptide derived from human RhoA |
| Gene ID number: |
387, 389 |
| Swiss Prot: |
P61586 |
| Tested applications: |
IF(ICC), IF(IHC-P), FCM |
| Recommended dilutions: |
IF(ICC)(1:50-200), IF(IHC-P)(1:50-200), FCM(1:20-100) |
| Crossreactivity: |
Mouse, Rat, Human |
| Crossreactive species details: |
not every possible cross-reactivity is known, Due to limited amount of testing and knowledge |
| Antigen background: |
Activated by ARHGEF2, Essential for the SPATA13-mediated regulation of cell migration and adhesion assembly and disassembly, In turn, Involved in a microtubule-dependent signal that is required for the myosin contractile ring formation during cell cycle cytokinesis, It controls the localization of APC and CLASP2 to the cell membrane, May be an activator of PLCE1, Plays an essential role in cleavage furrow formation, Required for the apical junction formation of keratinocyte cell-cell adhesion, Serves as a target for the yopT cysteine peptidase from Yersinia pestis, Stimulates PKN2 kinase activity, The MEMO1-RHOA-DIAPH1 signaling pathway plays an important role in ERBB2-dependent stabilization of microtubules at the cell cortex, and Yersinia pseudotuberculosis, membrane-bound APC allows the localization of the MACF1 to the cell membrane, vector of the plague, via the regulation of GSK3B activity, which causes gastrointestinal disorders, which is required for microtubule capture and stabilization, which promotes the exchange of GDP for GTP, Regulates a signal transduction pathway linking plasma membrane receptors to the assembly of focal adhesions and actin stress fibers |
| Purification method: |
This antibody was purified via Protein A |
| Storage conditions: |
50% glycerol and 0, Store refrigerated at 2 to 8 degrees Celcius for up to 1 year, 09% sodium azide, Keep the antibody in an aqueous buffered solution containing 1% BSA |
| Excitation Emission: |
494nm/518nm |
| Synonyms: |
ARH12, ARH9, ARHC, RHO12, RHOA, RHOH12, Rho cDNA clone 12, Rho cDNA clone 9, Rho-related GTP-binding protein RhoC, Transforming protein RhoA, h12, ARHA |
| Properties: |
Bioss Primary Conjugated Antibodies FITC is highly sensitive to pH extremes, FITC has a green emission that's usually collected at 530 nanometers, FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light, For fluorescent microscopy applications, This Bioss Primary Conjugated Antibodies Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS, When excited at 488 nanometers, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, the FL1 detector of a FACSCalibur or FACScan, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24° |
| Gene target: |
RhoA/C Conjugated |
| Short name: |
FITC Conjugated, RhoA/C Antibody |
| Technique: |
Fluorescein, antibodies against human proteins, antibodies for, antibody Conjugates, Antibody |
| Label: |
FITC |
| Alternative name: |
fluorecein coupled, ras homolog family member A/C (Antibody to) |
| Alternative technique: |
antibodies |