| Long name: |
FITC Conjugated, LC3A/B Polyclonal Antibody |
| Category: |
Conjugated Primary Antibodies |
| Conjugation: |
Anti-FITC Antibody |
| Host Organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Antigen: |
LC3A/B |
| Specificity: |
This is a highly specific antibody against LC3A/B |
| Modification: |
Unmodified |
| Modification site: |
None |
| Clonality: |
Polyclonal |
| Clone: |
Polyclonal antibody |
| Concentration: |
1ug per 1ul |
| Immunogen_range: |
27-59/121 |
| Subcellular location: |
Cytoplasm |
| Source: |
KLH conjugated synthetic peptide derived from human LC3A/B |
| Gene ID number: |
84557 |
| Swiss Prot: |
Q9H492 |
| Tested applications: |
IF(IHC-P) |
| Recommended dilutions: |
IF(IHC-P)(1:50-200) |
| Crossreactivity: |
Mouse, Rat, Human |
| Crossreactive species details: |
not every possible cross-reactivity is known, Due to limited amount of testing and knowledge |
| Antigen background: |
Whereas LC3s are involved in elongation of the phagophore membrane, the GABARAP/GATE-16 subfamily is essential for a later stage in autophagosome maturation, Ubiquitin-like modifier involved in formation of autophagosomal vacuoles (autophagosomes) |
| Purification method: |
This antibody was purified via Protein A |
| Storage conditions: |
50% glycerol and 0, Store refrigerated at 2 to 8 degrees Celcius for up to 1 year, 09% sodium azide, Keep the antibody in an aqueous buffered solution containing 1% BSA |
| Excitation Emission: |
494nm/518nm |
| Synonyms: |
MAP1BLC3 MAP1ALC3 LC3 LC3A ATG8E |
| Properties: |
Bioss Primary Conjugated Antibodies FITC is highly sensitive to pH extremes, FITC has a green emission that's usually collected at 530 nanometers, FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light, For fluorescent microscopy applications, This Bioss Primary Conjugated Antibodies Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS, When excited at 488 nanometers, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, the FL1 detector of a FACSCalibur or FACScan, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24° |
| Gene target: |
LC3A/B Conjugated |
| Short name: |
FITC Conjugated, LC3A/B Antibody |
| Technique: |
Fluorescein, antibodies against human proteins, antibodies for, antibody Conjugates, Antibody |
| Label: |
FITC |
| Alternative name: |
fluorecein coupled, LC3A/B (Antibody to) |
| Alternative technique: |
antibodies |
| Identity: |
6838 |
| Gene: |
MAP1LC3A |
More about : MAP1LC3A |
| Long gene name: |
microtubule associated protein 1 light chain 3 alpha |
| Locus: |
20q11, 22 |
| Discovery year: |
2000-06-29 |
| Entrez gene record: |
84557 |
| Pubmed identfication: |
8833088 17580304 |
| RefSeq identity: |
NM_181509 |
| Classification: |
Autophagy related |
| Havana BLAST/BLAT: |
OTTHUMG00000032306 |