| Long name: |
FITC Conjugated, GADD34 Polyclonal Antibody |
| Category: |
Conjugated Primary Antibodies |
| Conjugation: |
Anti-FITC Antibody |
| Host Organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Antigen: |
GADD34 |
| Specificity: |
This is a highly specific antibody against GADD34 |
| Modification: |
Unmodified |
| Modification site: |
None |
| Clonality: |
Polyclonal |
| Clone: |
Polyclonal antibody |
| Concentration: |
1ug per 1ul |
| Immunogen_range: |
415-450/674 |
| Subcellular location: |
Cytoplasm |
| Source: |
KLH conjugated synthetic peptide derived from human GADD34 |
| Gene ID number: |
23645 |
| Swiss Prot: |
O75807 |
| Tested applications: |
IF(IHC-P), FCM |
| Recommended dilutions: |
IF(IHC-P)(1:50-200), FCM(1:20-100) |
| Crossreactivity: |
Mouse, Rat, Human |
| Crossreactive species details: |
not every possible cross-reactivity is known, Due to limited amount of testing and knowledge |
| Antigen background: |
Down-regulates the TGF-beta signaling pathway by promoting dephosphorylation of TGFB1 by PP1, May promote apoptosis by inducing TP53 phosphorylation on 'Ser-15', thereby reversing the shut-off of protein synthesis initiated by stress-inducible kinases and facilitating recovery of cells from stress, Recruits the serine/threonine-protein phosphatase PP1 to dephosphorylate the translation initiation factor eIF-2A/EIF2S1 |
| Purification method: |
This antibody was purified via Protein A |
| Storage conditions: |
50% glycerol and 0, Store refrigerated at 2 to 8 degrees Celcius for up to 1 year, 09% sodium azide, Keep the antibody in an aqueous buffered solution containing 1% BSA |
| Excitation Emission: |
494nm/518nm |
| Synonyms: |
GADD34 |
| Properties: |
Bioss Primary Conjugated Antibodies FITC is highly sensitive to pH extremes, FITC has a green emission that's usually collected at 530 nanometers, FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light, For fluorescent microscopy applications, This Bioss Primary Conjugated Antibodies Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS, When excited at 488 nanometers, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, the FL1 detector of a FACSCalibur or FACScan, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24° |
| Gene target: |
GADD34 Conjugated |
| Short name: |
FITC Conjugated, GADD34 Antibody |
| Technique: |
Fluorescein, antibodies against human proteins, antibodies for, antibody Conjugates, Antibody |
| Label: |
FITC |
| Alternative name: |
fluorecein coupled, GADD34 (Antibody to) |
| Alternative technique: |
antibodies |
| Identity: |
14375 |
| Gene: |
PPP1R15A |
More about : PPP1R15A |
| Long gene name: |
protein phosphatase 1 regulatory subunit 15A |
| Synonyms gene name: |
protein phosphatase 1, regulatory (inhibitor) subunit 15A protein phosphatase 1, regulatory subunit 15A |
| Synonyms name: |
growth arrest and DNA-damage-inducible 34 |
| Locus: |
19q13, 2 |
| Discovery year: |
2001-06-29 |
| GenBank acession: |
U83981 |
| Entrez gene record: |
23645 |
| Pubmed identfication: |
9153226 9413226 |
| RefSeq identity: |
NM_014330 |
| Classification: |
Protein phosphatase 1 regulatory subunits |
| Havana BLAST/BLAT: |
OTTHUMG00000183330 |