| Long name: |
Biotin Conjugated, DC-SIGNR/CD299 Polyclonal Antibody |
| Category: |
Conjugated Primary Antibodies |
| Conjugation: |
Biotinylated |
| Host Organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Antigen: |
DC-SIGNR/CD299 |
| Specificity: |
This is a highly specific antibody against DC-SIGNR/CD299 |
| Modification: |
Unmodified |
| Modification site: |
None |
| Clonality: |
Polyclonal |
| Clone: |
Polyclonal antibody |
| Concentration: |
1ug per 1ul |
| Subcellular location: |
Extracellular |
| Source: |
KLH conjugated synthetic peptide derived from human CD299 |
| Gene ID number: |
10332 |
| Tested applications: |
IHC-P, WB |
| Recommended dilutions: |
IHC-P(1:100-500), WB(1:100-1000) |
| Crossreactivity: |
Human |
| Crossreactive species details: |
not every possible cross-reactivity is known, Due to limited amount of testing and knowledge |
| Antigen background: |
A key molecule, CD209L, CD299 is located on Human chromosome 19p13, CD299 is polymorphic, CD299 is primarily transcribed in the liver and lymph nodes but not in monocyte derived DC, DC SIGN2, Efforts to identify additional type II membrane proteins resulted in the isolation of a related molecule named CD299, Expression of CD299 is restricted to endothelial cells derived from liver sinusoids, Its structure is similar to DC SIGN1 and therefore binds mannose residues in a calcium dependent fashion, This is further supported by a study demonstrating the ability to isolate a large repertoire of CD299 transcripts largely the result of alternative splicing of the 7 coding exons, also known as DC SIGNR (DC SIGN Related), although variable expression in placenta and some monocytic cell lines has also been reported, and L SIGN (liver/lymph nodespecific ICAM3 grabbing nonintegrin), and capillaries, dendritic cell specific ICAM3 grabbing nonintegrin (DC SIGN1), including ICAM3 and HIV 1 gp120, including antigen presentation to T cells and enhancement of transinfection of CD4+ T cells by HIV 1, including both membrane and soluble isoforms of the protein, is involved in regulating interactions between DC and resting T cells, lymph nodes sinuses, since allelic variations of the exon 4 encoded sequence have been isolated, 3 and shares 73-80% amino acid identity with DC SIGN1, Dendritic cells (DC) play a primary role in the immune system as antigen presenting cells |
| Purification method: |
This antibody was purified via Protein A |
| Storage conditions: |
50% glycerol and 0, Store refrigerated at 2 to 8 degrees Celcius for up to 1 year, 09% sodium azide, Keep the antibody in an aqueous buffered solution containing 1% BSA |
| Synonyms: |
HP10347 DC-SIGNR LSIGN DCSIGNR DC-SIGN2 |
| Properties: |
Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA, Biotin conjugates can be detected by horseradish peroxidase, To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1, alkaline phosphatase substrates or anti biotin conjugated antibodies, 6, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24° |
| Gene target: |
DC-SIGNR/CD299 Conjugated |
| Short name: |
Conjugated, DC-SIGNR/CD299 Antibody |
| Technique: |
antibodies against human proteins, antibodies for, antibody Conjugates, Antibody |
| Label: |
Biotin |
| Alternative name: |
biotinilated coupled, DC-SIGNR/CD299 (Antibody to) |
| Alternative technique: |
antibodies |
| Identity: |
13523 |
| Gene: |
CLEC4M |
More about : CLEC4M |
| Long gene name: |
C-type lectin domain family 4 member M |
| Synonyms gene: |
CD209L CD299 |
| Synonyms gene name: |
CD299 antigen C-type lectin domain family 4, member M |
| Locus: |
19p13, 2 |
| Discovery year: |
2000-09-19 |
| GenBank acession: |
AB015629 |
| Entrez gene record: |
10332 |
| Pubmed identfication: |
10072769 |
| RefSeq identity: |
NM_014257 |
| Classification: |
CD molecules C-type lectin domain family |
| Havana BLAST/BLAT: |
OTTHUMG00000182432 |