| Long name: |
FITC Conjugated, E cadherin Polyclonal Antibody |
| Category: |
Conjugated Primary Antibodies |
| Conjugation: |
Anti-FITC Antibody |
| Host Organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Antigen: |
E cadherin |
| Specificity: |
This is a highly specific antibody against E cadherin |
| Modification: |
Unmodified |
| Modification site: |
None |
| Clonality: |
Polyclonal |
| Clone: |
Polyclonal antibody |
| Concentration: |
1ug per 1ul |
| Immunogen_range: |
410-460/882 |
| Subcellular location: |
Extracellular |
| Source: |
KLH conjugated synthetic peptide derived from human E-cadherin |
| Gene ID number: |
999 |
| Swiss Prot: |
P12830 |
| Tested applications: |
IF(ICC), IF(IHC-P), FCM |
| Recommended dilutions: |
IF(ICC)(1:50-200), IF(IHC-P)(1:50-200), FCM(1:20-100) |
| Crossreactivity: |
Mouse, Rat, Human |
| Crossreactive species details: |
not every possible cross-reactivity is known, Due to limited amount of testing and knowledge |
| Antigen background: |
CDH1 is involved in mechanisms regulating cell-cell adhesions, E-Cad/CTF2 promotes non-amyloidogenic degradation of Abeta precursors, Has a potent invasive suppressor role, Has a strong inhibitory effect on APP C99 and C83 production, It is a ligand for integrin alpha-E/beta-7, They preferentially interact with themselves in a homophilic manner in connecting cells, cadherins may thus contribute to the sorting of heterogeneous cell types, mobility and proliferation of epithelial cells, Cadherins are calcium-dependent cell adhesion proteins |
| Purification method: |
This antibody was purified via Protein A |
| Storage conditions: |
50% glycerol and 0, Store refrigerated at 2 to 8 degrees Celcius for up to 1 year, 09% sodium azide, Keep the antibody in an aqueous buffered solution containing 1% BSA |
| Excitation Emission: |
494nm/518nm |
| Synonyms: |
Arc-1, CAM 12/8, CD324, CDH1, CDHE, Cadherin-1, E-cadherin, ECAD, Epithelial cadherin, LCAM, Uvomorulin, UVO |
| Properties: |
Bioss Primary Conjugated Antibodies FITC is highly sensitive to pH extremes, FITC has a green emission that's usually collected at 530 nanometers, FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light, For fluorescent microscopy applications, This Bioss Primary Conjugated Antibodies Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS, When excited at 488 nanometers, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, the FL1 detector of a FACSCalibur or FACScan, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24° |
| Gene target: |
E cadherin Conjugated |
| Short name: |
FITC Conjugated, E cadherin Antibody |
| Technique: |
Fluorescein, antibodies against human proteins, antibodies for, antibody Conjugates, Antibody |
| Label: |
FITC |
| Alternative name: |
fluorecein coupled, E cadherin (Antibody to) |
| Alternative technique: |
antibodies |