| Long name: |
HSP27 Primary Polyclonal Antibody |
| Also known as: |
HSP27 PAb |
| Category: |
Primary Antibodies |
| Conjugation: |
Unconjugated |
| Target Antigen: |
HSP27 |
| Specificity: |
This is a highly specific antibody against HSP27 |
| Modification(s): |
None |
| Modification site(s): |
Unmodified antibody |
| Clonality: |
Polyclonal antibody |
| Clone number: |
Polyclonal antibody |
| Concentration: |
1ug per 1ul |
| Immunogen range: |
100-150/205 |
| Subcellular location: |
Nucleus, Cytoplasm |
| Source: |
This antibody was obtained by immunization of the host with KLH conjugated synthetic peptide derived from human HSP27 |
| Gene ID number: |
3315 |
| Swiss Prot: |
P04792 |
| Tested Applications: |
IF(IHC-P), IHC-P, WB |
| Recommended dilutions: |
IF(IHC-P)(1:50-200), IHC-P(1:100-500), WB(1:100-1000) |
| Cross reactivity: |
Mouse, Rat, Human |
| Cross reactive species details: |
not every possible cross-reactivity is known, Due to limited amount of testing and knowledge |
| Background of the target antigen: |
A major function of these proteins is to serve as chaperones that bind to and stabilize the active conformation of other proteins, Akt (PKB), Another complex consists of HSP27 and the IKK complex, Cytoplasmic HSP27 exists in multiple complexes, HSP27, HSP27 is also an actin capping protein that binds to the barbed (growing) ends of actin filaments, HSP27 is localized to the cytoplasm of unstressed cells but can redistribute to the nucleus in response to stress, MAPKAP-kinase 2, One complex consists of HSP27, Phosphorylation of HSP27 on serine 82 by MAPKAP-kinase 2 leads to HSP27 dissociation from the Akt/MAPKAP-kinase 2/p38 complex and from actin filaments, The presence of HSP27 in this complex is required for Akt activation by stress stimuli, along with other members of the small HSP group, and p38 MAPK, and stimulates HSP27 binding to the IKK complex, possesses a C-terminal Alpha-crystalline homology domain, thereby inhibiting filament extension, where it may function to stabilize DNA and/or the nuclear membrane, Heat Shock Protein 27 (HSP27) is a 27 kDa member of a family of proteins whose expression and function are stimulated by heat shock and other stress stimuli |
| Purification method: |
This antibody was purified via Protein A |
| Storage conditions: |
50% glycerol and 0, Store at -20°, 09% sodium azide, C for up to 1 year, Keep the antibody in aqueous buffered solution containing 1% BSA |
| Synonym names: |
28 kDa heat shock protein, Estrogen-regulated 24 kDa protein, HEL-S-12, HMN2B, HS, HSP 27, HSP27, HSP28, Heat shock 27 kDa protein, Heat shock protein beta-1, Hsp25, HspB1, SRP27, Stress-responsive protein 27, 7667, CMT2F |
| Properties: |
C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Unconjugated Antibodies they should be stored frozen at - 24° |
| Gene target: |
HSP27 |
| Short name: |
HSP27 Antibody |
| Technique: |
antibodies against human proteins, antibodies for, Antibody |
| Alternative name: |
HSP27 (Antibody to) |
| Alternative technique: |
antibodies |
| Identity: |
5246 |
| Gene: |
HSPB1 |
More about : HSPB1 |
| Long gene name: |
heat shock protein family B (small) member 1 |
| Synonyms gene name: |
heat shock 27kD protein 1 heat shock 27kDa protein 1 |
| Synonyms: |
HSP27 HSP28 Hs, 76067 Hsp25 CMT2F |
| Locus: |
7q11, 23 |
| Discovery year: |
1991-07-09 |
| GenBank acession: |
X54079 |
| Entrez gene record: |
3315 |
| Pubmed identfication: |
2243808 9344682 |
| Classification: |
Small heat shock proteins |
| Havana BLAST/BLAT: |
OTTHUMG00000023228 |
| Locus Specific Databases: |
Inherited Peripheral Neuropathies Mutation Database LRG_248 |