| Long name: |
ALEXA FLUOR 350 Conjugated, Cyclin D1 Polyclonal Antibody |
| Also known as: |
Anti-Cyclin D1 PAb ALEXA FLUOR 350 |
| Category: |
Conjugated Primary Antibodies |
| Conjugated with: |
350, ALEXA FLUOR® |
| Host Organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Antigen: |
Cyclin D1 |
| Specificity: |
This is a highly specific antibody against Cyclin D1 |
| Modification: |
Unmodified |
| Modification Site: |
None |
| Clonality: |
Polyclonal |
| Clone: |
Polyclonal antibody |
| Concentration: |
1ug per 1ul |
| Immunogen range: |
70-120/295 |
| Subcellular location: |
Nucleus, Cytoplasm |
| Source: |
KLH conjugated synthetic peptide derived from mouse Cyclin D1 |
| Gene ID Number: |
12443 |
| Swiss Prot: |
P25322 |
| Tested applications: |
IF(ICC), IF(IHC-P), FCM |
| Recommended dilutions: |
IF(ICC)(1:50-200), IF(IHC-P)(1:50-200), FCM(1:20-100) |
| Crossreactivity: |
Goat, Mouse, Rat, Human |
| Cross-reactive species details: |
not every possible cross-reactivity is known, Due to limited amount of testing and knowledge |
| Background of the antigen: |
Also substrate for SMAD3, Component of the ternary complex, Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals, Exhibits transcriptional corepressor activity with INSM1 on the NEUROD1 and INS promoters in a cell cycle-independent manner (By similarity), Hypophosphorylates RB1 in early G(1) phase, Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complex and the subsequent transcription of E2F target genes which are responsible for the progression through the G(1) phase, cyclin D1/CDK4/CDKN1B, phosphorylating SMAD3 in a cell-cycle-dependent manner and repressing its transcriptional activity, required for nuclear translocation and activity of the cyclin D-CDK4 complex, Regulatory component of the cyclin D1-CDK4 (DC) complex that phosphorylates and inhibits members of the retinoblastoma (RB) protein family including RB1 and regulates the cell-cycle during G(1)/S transition |
| Purification: |
Purified by Protein A |
| Storage conditions: |
50% glycerol and 0, Keep refrigerated at 2 to 8 degrees Celcius for up to one year, 09% sodium azide, Store this antibody in aqueous buffered solution containing 1% BSA |
| Excitation emission: |
343nm/442nm |
| Synonyms: |
U21B31 |
| Properties: |
ALEXA FLUOR they should be stored frozen at - 24°, Alexa Fluor 350 conjugates can be used in multi-color flow cytometry with FACS's equipped with a second red laser or red diode, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies, C, C for long term storage and for short term at + 5°, For facs or microscopy Alexa 1 conjugate |
| Conjugation: |
ALEXA FLUOR 350, Alexa Fluor |
| Gene target: |
Cyclin D1 |
| Short name: |
Cyclin D1 Antibody |
| Technique: |
antibodies against human proteins, antibodies for, Antibody |
| Label: |
ALEXA FLUOR 350 |
| Alternative name: |
ALEXA FLUOR 350, Cyclin D1 (Antibody to) |
| Alternative technique: |
antibodies |
| Identity: |
1582 |
| Gene: |
CCND1 |
More about : CCND1 |
| Long gene name: |
cyclin D1 |
| Synonyms gene: |
BCL1 D11S287E PRAD1 |
| Synonyms gene name: |
cyclin D1 (PRAD1: parathyroid adenomatosis 1) |
| Synonyms name: |
parathyroid adenomatosis 1 B-cell CLL/lymphoma 1 G1/S-specific cyclin D1 |
| Locus: |
11q13, 3 |
| Discovery year: |
1991-06-06 |
| GenBank acession: |
Z23022 |
| Entrez gene record: |
595 |
| Pubmed identfication: |
1826542 1833066 |
| RefSeq identity: |
NM_053056 |
| Classification: |
Cyclins |
| Havana BLAST/BLAT: |
OTTHUMG00000167877 |
| Locus Specific Databases: |
LRG_990 |