| Reacts with: |
Human |
| Source: |
proteins, Recombinants or rec |
| Description: |
Recombinant Human Hemoglobin subunit alpha is produced by our E, coli expression system and the target gene encoding Met1-Arg142 is expressed with a 6His tag at the N-terminus |
| Molecular Weight: |
16, 7 kD |
| UniProt number: |
P69905 |
| State of the product: |
Freeze-dried |
| Shipping conditions: |
Ambient temperature |
| Formulation: |
150 mM sodium chloride, pH 7, 2 um filtered solution of 20 mM PB, Lyophilized from a 0 |
| Storage recommendations: |
-20°, Aliquots of reconstituted samples are stable at less -20°, If kept at at room temperature, If kept at at room temperature, Reconstituted protein solution should be stored at 4-7°, it is stable for 3 weeks, it is stable for 3 weeks, C, C for 2-7 days, C for 3 months, Freeze-dried proteins should be stored frozen at < |
| Reconstitution: |
Dissolve the lyophilized protein in ddH2O, Do not mix by vortex or pipetting, It is not recommended to reconstitute to a concentration less than 100 &mu, Please aliquot the reconstituted solution to minimize freeze-thaw cycles, Always centrifuge tubes before opening, g/ml |
| Purity: |
and determined by Polyacrylamide gel electrophoresis, Greater than 95% |
| Sequence of the amino acid: |
MNHKVHHHHHHMVLSPADKTNVKAAWGKVGAHAGEYGAEALERMFLSFPTTKTYFPHFDLSHGSAQVKGHGKKVADALTNAVAHVDDMPNALSALSDLHAHKLRVDPVNFKLLSHCLLVTLAAHLPAEFTPAVHASLDKFLASVSTVLTSKYR |
| Levels of endotoxin: |
11 IEU/ug, LAL test shows less than than 0 |
| Properties: |
Depending on the epitopes used human ELISA kits can be cross reactive to many other species, Mainly analyzed are human serum, Modern , cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies, human cell culture supernatants and biological samples, plasma, primarily , saliva, urine, , (Homo sapiens, Homo sapiens sapiens), Human proteins, humans , ssp |
| Group: |
recombinants |
| Gene target: |
HBA1 (N-6His), Hemoglobin Subunit &alpha |
| Short name: |
HBA1 (N-6His), Recombinant Hemoglobin Subunit &alpha |
| Technique: |
E, coli recombinant proteins are , novo advises they will be reconstituted in a buffer soluion or culture medium for cell culture, supplied as white sterile powder lyopillized, Recombinant, genetic recombinations , in Escherichia coli |
| Species: |
Humans |
| Alternative name: |
alpha 1 (N-6His), hemoglobin, sapiens Hemoglobin functionnal sequence &alpha, recombinant H |
| Alternative technique: |
rec |
| Alternative to gene target: |
Extracellular, HBA-T2 and HBA-T3 and HBH, HBA1 and IDBG-7127 and ENSG00000206172 and 3039, alpha 1, haptoglobin binding, this GO :0004601 and peroxidase activity and molecular function this GO :0005344 and oxygen transporter activity and molecular function this GO :0005506 and iron ion binding and molecular function this GO :0005515 and protein binding and molecular function this GO :0005576 and extracellular region and cellular component this GO :0005829 and cytosol and cellular component this GO :0005833 and hemoglobin complex and cellular component this GO :0010942 and positive regulation of cell death and biological process this GO :0015671 and oxygen transport and biological process this GO :0015701 and bicarbonate transport and biological process this GO :0016020 and membrane and cellular component this GO :0019825 and oxygen binding and molecular function this GO :0020037 and heme binding and molecular function this GO :0022627 and cytosolic small ribosomal subunit and cellular component this GO :0031720 and haptoglobin binding and molecular function this GO :0031838 and haptoglobin-hemoglobin complex and cellular component this GO :0042542 and response to hydrogen peroxide and biological process this GO :0042744 and hydrogen peroxide catabolic process and biological process this GO :0044281 and small molecule metabolic process and biological process this GO :0051291 and protein heterooli this GO merization and biological process this GO :0055114 and oxidation-reduction process and biological process this GO :0070062 and extracellular vesicular exosome and cellular component this GO :0071682 and endocytic vesicle lumen and cellular component this GO :0072562 and blood microparticle and cellular component, this GO :0004601 : peroxidase activity, this GO :0004601 : peroxidase activity and also this GO :0005344 : oxygen transporter activity and also this GO :0005506 : iron ion binding and also this GO :0005515 : protein binding and also this GO :0019825 : oxygen binding and also this GO :0020037 : heme binding and also this GO :0031720 : haptoglobin binding, this GO :0005344 : oxygen transporter activity, this GO :0005506 : iron ion binding, this GO :0005515 : protein binding, this GO :0019825 : oxygen binding, this GO :0020037 : heme binding, this GO :0031720 : haptoglobin binding, 3040, hemoglobin |
| Identity: |
4823 |
| Gene: |
HBA1 |
More about : HBA1 |
| Long gene name: |
hemoglobin subunit alpha 1 |
| Synonyms gene name: |
alpha 1 , hemoglobin |
| Synonyms: |
HBA-T3 |
| Locus: |
16p13, 3 |
| Discovery year: |
2001-06-22 |
| GenBank acession: |
AF349571 |
| Entrez gene record: |
3039 |
| Pubmed identfication: |
1975428 2649166 |
| RefSeq identity: |
NM_000558 |
| Classification: |
Hemoglobin subunits |
| Havana BLAST/BLAT: |
OTTHUMG00000060138 |
| Locus Specific Databases: |
HbVar: A Database of Human Hemoglobin Variants and Thalassemias The Globin Gene Server |