| Reacts with: |
Human |
| Source: |
proteins, Recombinants or rec |
| Description: |
Recombinant Human Neutrophil cytosol factor 1 is produced by our E, coli expression system and the target gene encoding Met1-Val390 is expressed |
| Molecular Weight: |
45, 6 kD |
| UniProt number: |
P14598 |
| State of the product: |
Freeze-dried |
| Shipping conditions: |
Ambient temperature |
| Formulation: |
1 mM DTT, 1M sodium chloride, 2 um filtered solution of 20 mM Tris pH8, Lyophilized from a 0 |
| Storage recommendations: |
-20°, Aliquots of reconstituted samples are stable at less -20°, If kept at at room temperature, If kept at at room temperature, Reconstituted protein solution should be stored at 4-7°, it is stable for 3 weeks, it is stable for 3 weeks, C, C for 2-7 days, C for 3 months, Freeze-dried proteins should be stored frozen at < |
| Reconstitution: |
Dissolve the lyophilized protein in ddH2O, Do not mix by vortex or pipetting, It is not recommended to reconstitute to a concentration less than 100 &mu, Please aliquot the reconstituted solution to minimize freeze-thaw cycles, Always centrifuge tubes before opening, g/ml |
| Purity: |
and determined by Polyacrylamide gel electrophoresis, Greater than 95% |
| Sequence of the amino acid: |
MGDTFIRHIALLGFEKRFVPSQHYVYMFLVKWQDLSEKVVYRRFTEIYEFHKTLKEMFPIEAGAINPENRIIPHLPAPKWFDGQRAAENRQGTLTEYCSTLMSLPTKISRCPHLLDFFKVRPDDLKLPTDNQTKKPETYLMPKDGKSTATDITGPIILQTYRAIANYEKTSGSEMALSTGDVVEVVEKSESGWWFCQMKAKRGWIPASFLEPLDSPDETEDPEPNYAGEPYVAIKAYTAVEGDEVSLLEGEAVEVIHKLLDGWWVIRKDDVTGYFPSMYLQKSGQDVSQAQRQIKRGAPPRRSSIRNAHSIHQRSRKRLSQDAYRRNSVRFLQQRRRQARPGPQSPGSPLEEERQTQRSKPQPAVPPRPSADLILNRCSESTKRKLASAVEHHHHHH |
| Levels of endotoxin: |
11 IEU/ug, LAL test shows less than than 0 |
| Properties: |
Depending on the epitopes used human ELISA kits can be cross reactive to many other species, Mainly analyzed are human serum, Modern , cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies, human cell culture supernatants and biological samples, plasma, primarily , saliva, urine, , (Homo sapiens, Homo sapiens sapiens), Human proteins, humans , ssp |
| Group: |
recombinants |
| Gene target: |
NCF1, Neutrophil Cytosol Factor 1 |
| Short name: |
NCF1, Recombinant Neutrophil Cytosol Factor 1 |
| Technique: |
E, coli recombinant proteins are , novo advises they will be reconstituted in a buffer soluion or culture medium for cell culture, supplied as white sterile powder lyopillized, Recombinant, genetic recombinations , in Escherichia coli |
| Species: |
Humans, Human |
| Alternative name: |
neutrophil cytosolic factor 1, sapiens Neutrophil cytoplasm Factor 1, recombinant H |
| Alternative technique: |
rec |
| Alternative to gene target: |
NCF1 and IDBG-21570 and ENSG00000158517 and 653361, NCF1 and IDBG-639883 and ENSBTAG00000003305 and 281345, Ncf1 and IDBG-202592 and ENSMUSG00000015950 and 17969, Plasma membranes, TAP-dependent and biological process this GO :0002679 and respiratory burst involved in defense response and biological process this GO :0005515 and protein binding and molecular function this GO :0005737 and cytoplasm and cellular component this GO :0005791 and rough endoplasmic reticulum and cellular component this GO :0005794 and this GO lgi apparatus and cellular component this GO :0005829 and cytosol and cellular component this GO :0005886 and plasma membrane and cellular component this GO :0006612 and protein targeting to membrane and biological process this GO :0006691 and leukotriene metabolic process and biological process this GO :0006801 and superoxide metabolic process and biological process this GO :0006915 and apoptotic process and biological process this GO :0006954 and inflammatory response and biological process this GO :0006968 and cellular defense response and biological process this GO :0008283 and cell proliferation and biological process this GO :0009055 and electron carrier activity and molecular function this GO :0009617 and response to bacterium and biological process this GO :0016020 and membrane and cellular component this GO :0016175 and superoxide-generating NADPH oxidase activity and molecular function this GO :0017124 and SH3 domain binding and molecular function this GO :0019898 and extrinsic component of membrane and cellular component this GO :0030425 and dendrite and cellular component this GO :0032010 and pha this GO lysosome and cellular component this GO :0035091 and phosphatidylinositol binding and molecular function this GO :0042554 and superoxide anion generation and biological process this GO :0042590 and antigen processing and presentation of exogenous peptide antigen via MHC class I and biological process this GO :0042742 and defense response to bacterium and biological process this GO :0043020 and NADPH oxidase complex and cellular component this GO :0043025 and neuronal cell body and cellular component this GO :0043325 and phosphatidylinositol-3, phosphatidylinositol-3, this GO :0001878 and response to yeast and biological process this GO :0001909 and leukocyte mediated cytotoxicity and biological process this GO :0002474 and antigen processing and presentation of peptide antigen via MHC class I and biological process this GO :0002479 and antigen processing and presentation of exogenous peptide antigen via MHC class I, this GO :0005515 : protein binding, this GO :0005515 : protein binding and also this GO :0009055 : electron carrier activity and also this GO :0016175 : superoxide-generating NADPH oxidase activity and also this GO :0017124 : SH3 domain binding and also this GO :0035091 : phosphatidylinositol binding and also this GO :0043325 : phosphatidylinositol-3, this GO :0009055 : electron carrier activity, this GO :0016175 : superoxide-generating NADPH oxidase activity, this GO :0017124 : SH3 domain binding, this GO :0035091 : phosphatidylinositol binding, this GO :0043325 : phosphatidylinositol-3, 4-bisphosphate binding, 4-bisphosphate binding, 4-bisphosphate binding and molecular function this GO :0045087 and innate immune response and biological process this GO :0045730 and respiratory burst and biological process this GO :0045986 and negative regulation of smooth muscle contraction and biological process this GO :0050665 and hydrogen peroxide biosynthetic process and biological process this GO :0050830 and defense response to Gram-positive bacterium and biological process this GO :0050832 and defense response to fungus and biological process this GO :0051701 and interaction with host and biological process this GO :0055114 and oxidation-reduction process and biological process this GO :0070946 and neutrophil mediated killing of gram-positive bacterium and biological process this GO :0070947 and neutrophil mediated killing of fungus and biological process this GO :0090382 and pha this GO some maturation and biological process, neutrophil cytosolic factor 1 |
| Identity: |
7660 |
| Gene: |
NCF1 |
More about : NCF1 |
| Long gene name: |
neutrophil cytosolic factor 1 |
| Synonyms gene name: |
autosomal 1) , chronic granulomatous disease, neutrophil cytosolic factor 1 (47kD |
| Synonyms: |
p47phox NOXO2 NCF1A SH3PXD1A |
| Synonyms name: |
NADPH oxidase organizer 2 chronic granulomatous disease, autosomal 1 |
| Locus: |
7q11, 23 |
| Discovery year: |
1989-05-25 |
| GenBank acession: |
M25665 |
| Entrez gene record: |
653361 |
| RefSeq identity: |
NM_000265 |
| Havana BLAST/BLAT: |
OTTHUMG00000149965 |
| Locus Specific Databases: |
NCF1base: Mutation registry for autosomal recessive chronic granulomatous disease (CGD), deficiency of p47phox LRG_87 |