| Reacts with: |
Human |
| Source: |
proteins, Recombinants or rec |
| Description: |
Recombinant Human Valosin-Containing Protein is produced by our E, coli expression system and the target gene encoding Ala2-Asn589 is expressed with a 6His tag at the C-terminus |
| Molecular Weight: |
4 kD, 66 |
| UniProt number: |
P55072 |
| State of the product: |
Liquid |
| Shipping conditions: |
Dry Ice/ice packs |
| Formulation: |
2 um filtered solution of 50 mM TrisHCl，pH8, Supplied as a 0 |
| Storage recommendations: |
-20°, Please minimize freeze-thaw cycles, stable for 6 months after receipt, C, Store at < |
| Reconstitution: |
Dissolve the lyophilized protein in ddH2O, Do not mix by vortex or pipetting, It is not recommended to reconstitute to a concentration less than 100 &mu, Please aliquot the reconstituted solution to minimize freeze-thaw cycles, Always centrifuge tubes before opening, g/ml |
| Purity: |
and determined by Polyacrylamide gel electrophoresis, Greater than 95% |
| Sequence of the amino acid: |
ASGADSKGDDLSTAILKQKNRPNRLIVDEAINEDNSVVSLSQPKMDELQLFRGDTVLLKGKKRREAVCIVLSDDTCSDEKIRMNRVVRNNLRVRLGDVISIQPCPDVKYGKRIHVLPIDDTVEGITGNLFEVYLKPYFLEAYRPIRKGDIFLVRGGMRAVEFKVVETHPSPYCIVAPDTVIHCEGEPIKREDEEESLNEVGYDDIGGCRKQLAQIKEMVELPLRHPALFKAIGVKPPRGILLYGPPGTGKTLIARAVANETGAFFFLINGPEIMSKLAGESESNLRKAFEEAEKNAPAIIFIDELDAIAPKREKTHGEVERRIVSQLLTLMDGLKQRAHVIVMAATNRPNSIDPALRRFGRFDREVDIGIPDATGRLEILQIHTKNMKLADDVDLEQVANETHGHVGADLAALCSEAALQAIRKKMDLIDLEDETIDAEVMNSLAVTMDDFRWALSQSNPSALRETVVEVPQVTWEDIGGLEDVKRELQELVQYPVEHPDKFLKFGMTPSKGVLFYGPPGCGKTLLAKAIANECQANFISIKGPELLTMWFGESEANVREIFDKARQAAPCVLFFDELDSIAKARGGNVEHHHHHH |
| Levels of endotoxin: |
11 IEU/ug, LAL test shows less than than 0 |
| Properties: |
Depending on the epitopes used human ELISA kits can be cross reactive to many other species, Mainly analyzed are human serum, Modern , cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies, human cell culture supernatants and biological samples, plasma, primarily , saliva, urine, , (Homo sapiens, Homo sapiens sapiens), Human proteins, humans , ssp |
| Group: |
recombinants |
| Gene target: |
VCP (C-6His), Valosin Protein |
| Short name: |
VCP (C-6His), Recombinant Valosin- Protein |
| Technique: |
E, coli recombinant proteins are , novo advises they will be reconstituted in a buffer soluion or culture medium for cell culture, supplied as white sterile powder lyopillized, Recombinant, genetic recombinations , in Escherichia coli |
| Species: |
Humans, Human |
| Alternative name: |
sapiens Valosin-Containing Protein, valosin containing protein (C-6His), recombinant H |
| Alternative technique: |
rec |
| Alternative to gene target: |
ALS14 and HEL-220 and HEL-S-70 and IBMPFD and IBMPFD1 and p97 and TERA, BT, ER to cytosol and biological process this GO :0031334 and positive regulation of protein complex assembly and biological process this GO :0031593 and polyubiquitin binding and molecular function this GO :0032403 and protein complex binding and molecular function this GO :0032436 and positive regulation of proteasomal ubiquitin-dependent protein catabolic process and biological process this GO :0034214 and protein hexamerization and biological process this GO :0035800 and deubiquitinase activator activity and molecular function this GO :0035861 and site of double-strand break and cellular component this GO :0042802 and identical protein binding and molecular function this GO :0042981 and regulation of apoptotic process and biological process this GO :0043161 and proteasome-mediated ubiquitin-dependent protein catabolic process and biological process this GO :0043231 and intracellular membrane-bounded organelle and cellular component this GO :0043234 and protein complex and cellular component this GO :0043531 and ADP binding and molecular function this GO :0044822 and poly(A) RNA binding and molecular function this GO :0045184 and establishment of protein localization and biological process this GO :0045732 and positive regulation of protein catabolic process and biological process this GO :0048471 and perinuclear region of cytoplasm and cellular component this GO :0051260 and protein homooli this GO merization and biological process this GO :0070062 and extracellular vesicular exosome and cellular component this GO :0070842 and aggresome assembly and biological process this GO :1903006 and positive regulation of protein K63-linked deubiquitination and biological process this GO :1903007 and positive regulation of Lys63-specific deubiquitinase activity and biological process this GO :1990381 and ubiquitin-specific protease binding and molecular function, VCP and IDBG-61415 and ENSG00000165280 and 7415, Vcp and IDBG-139755 and ENSMUSG00000028452 and 269523, nuclei, this GO :0000502 and proteasome complex and cellular component this GO :0000836 and Hrd1p ubiquitin ligase complex and cellular component this GO :0005102 and receptor binding and molecular function this GO :0005515 and protein binding and molecular function this GO :0005524 and ATP binding and molecular function this GO :0005634 and nucleus and cellular component this GO :0005737 and cytoplasm and cellular component this GO :0005783 and endoplasmic reticulum and cellular component this GO :0005789 and endoplasmic reticulum membrane and cellular component this GO :0005811 and lipid particle and cellular component this GO :0005829 and cytosol and cellular component this GO :0006200 and ATP catabolic process and biological process this GO :0006281 and DNA repair and biological process this GO :0006302 and double-strand break repair and biological process this GO :0006310 and DNA recombination and biological process this GO :0006511 and ubiquitin-dependent protein catabolic process and biological process this GO :0006888 and ER to this GO lgi vesicle-mediated transport and biological process this GO :0006919 and activation of cysteine-type endopeptidase activity involved in apoptotic process and biological process this GO :0006974 and cellular response to DNA damage stimulus and biological process this GO :0008289 and lipid binding and molecular function this GO :0009378 and four-way junction helicase activity and molecular function this GO :0016567 and protein ubiquitination and biological process this GO :0016787 and hydrolase activity and molecular function this GO :0016887 and ATPase activity and molecular function this GO :0018279 and protein N-linked glycosylation via asparagine and biological process this GO :0019903 and protein phosphatase binding and molecular function this GO :0019904 and protein domain specific binding and molecular function this GO :0019985 and translesion synthesis and biological process this GO :0030433 and ER-associated ubiquitin-dependent protein catabolic process and biological process this GO :0030968 and endoplasmic reticulum unfolded protein response and biological process this GO :0030970 and retrograde protein transport, this GO :0005102 : receptor binding, this GO :0005102 : receptor binding and also this GO :0005515 : protein binding and also this GO :0005524 : ATP binding and also this GO :0008289 : lipid binding and also this GO :0009378 : four-way junction helicase activity and also this GO :0016787 : hydrolase activity and also this GO :0016887 : ATPase activity and also this GO :0019903 : protein phosphatase binding and also this GO :0019904 : protein domain specific binding and also this GO :0031593 : polyubiquitin binding and also this GO :0032403 : protein complex binding and also this GO :0035800 : deubiquitinase activator activity and also this GO :0042802 : identical protein binding and also this GO :0043531 : ADP binding and also this GO :0044822 : poly(A) RNA binding and also this GO :1990381 : ubiquitin-specific protease binding, this GO :0005515 : protein binding, this GO :0005524 : ATP binding, this GO :0008289 : lipid binding, this GO :0009378 : four-way junction helicase activity, this GO :0016787 : hydrolase activity, this GO :0016887 : ATPase activity, this GO :0019903 : protein phosphatase binding, this GO :0019904 : protein domain specific binding, this GO :0031593 : polyubiquitin binding, this GO :0032403 : protein complex binding, this GO :0035800 : deubiquitinase activator activity, this GO :0042802 : identical protein binding, this GO :0043531 : ADP binding, this GO :0044822 : poly(A) RNA binding, this GO :1990381 : ubiquitin-specific protease binding, ubiquitin-specific protease binding, 49331 and IDBG-632847 and ENSBTAG00000015000 and 507345, valosin containing protein |
| Identity: |
12666 |
| Gene: |
VCP |
More about : VCP |
| Long gene name: |
valosin containing protein |
| Synonyms gene name: |
valosin-containing protein |
| Synonyms: |
IBMPFD p97 CDC48 |
| Locus: |
9p13, 3 |
| Discovery year: |
1996-08-22 |
| GenBank acession: |
AC004472 |
| Entrez gene record: |
7415 |
| Pubmed identfication: |
8595912 7553851 |
| RefSeq identity: |
NM_007126 |
| Classification: |
AAA ATPases |
| Havana BLAST/BLAT: |
OTTHUMG00000019855 |
| Locus Specific Databases: |
LRG_657 |