| Reacts with: |
Human |
| Source: |
proteins, Recombinants or rec |
| Description: |
Recombinant Human Platelet-Activating Factor Acetylhydrolase is produced by our Mammalian expression system and the target gene encoding Phe22-Asn441 is expressed with a 6His tag at the C-terminus |
| Molecular Weight: |
48, 8 kD |
| UniProt number: |
Q13093 |
| State of the product: |
Liquid |
| Shipping conditions: |
Dry ice/ice packs |
| Formulation: |
10% Glycerol, 150 mM sodium chloride, pH 4, 2 um filtered solution of 20 mM HAc-sodium chloride, 5, Supplied as a 0 |
| Storage recommendations: |
-20°, Please minimize freeze-thaw cycles, stable for 6 months after receipt, C, Store at < |
| Reconstitution: |
Dissolve the lyophilized protein in ddH2O, Do not mix by vortex or pipetting, It is not recommended to reconstitute to a concentration less than 100 &mu, Please aliquot the reconstituted solution to minimize freeze-thaw cycles, Always centrifuge tubes before opening, g/ml |
| Purity: |
and determined by Polyacrylamide gel electrophoresis, Greater than 95% |
| Sequence of the amino acid: |
FDWQYINPVAHMKSSAWVNKIQVLMAAASFGQTKIPRGNGPYSVGCTDLMFDHTNKGTFLRLYYPSQDNDRLDTLWIPNKEYFWGLSKFLGTHWLMGNILRLLFGSMTTPANWNSPLRPGEKYPLVVFSHGLGAFRTLYSAIGIDLASHGFIVAAVEHRDRSASATYYFKDQSAAEIGDKSWLYLRTLKQEEETHIRNEQVRQRAKECSQALSLILDIDHGKPVKNALDLKFDMEQLKDSIDREKIAVIGHSFGGATVIQTLSEDQRFRCGIALDAWMFPLGDEVYSRIPQPLFFINSEYFQYPANIIKMKKCYSPDKERKMITIRGSVHQNFADFTFATGKIIGHMLKLKGDIDSNAAIDLSNKASLAFLQKHLGLHKDFDQWDCLIEGDDENLIPGTNINTTNQHIMLQNSSGIEKYNVDHHHHHH |
| Levels of endotoxin: |
11 IEU/ug, LAL test shows less than than 0 |
| Properties: |
Depending on the epitopes used human ELISA kits can be cross reactive to many other species, Mainly analyzed are human serum, Modern , cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies, human cell culture supernatants and biological samples, plasma, primarily , saliva, urine,  , (Homo sapiens, Homo sapiens sapiens), Human proteins, humans , ssp |
| Group: |
recombinants |
| Gene target: |
PLA2G7 (C-6His), PAF-AH |
| Short name: |
PLA2G7 (C-6His), Recombinant PAF-AH |
| Technique: |
E, coli recombinant proteins are , novo advises they will be reconstituted in a buffer soluion or culture medium for cell culture, supplied as white sterile powder lyopillized, Recombinant, genetic recombinations , in Escherichia coli |
| Species: |
Humans, Human |
| Alternative name: |
family VII (platelet-activating factor acetylhydrolase, phospholipase A2, plasma) (C-6His), sapiens PAF-AH, recombinant H |
| Alternative technique: |
rec |
| Alternative to gene target: |
Extracellular, LDL-PLA2 and LP-PLA2 and PAFAD and PAFAH, PLA2G7 and IDBG-632325 and ENSBTAG00000019315 and 282311, PLA2G7 and IDBG-89956 and ENSG00000146070 and 7941, Pla2g7 and IDBG-184295 and ENSMUSG00000023913 and 27226, calcium-independent phospholipase A2 activity, group VII (platelet-activating factor acetylhydrolase, plasma), this GO :0003847 and 1-alkyl-2-acetylglycerophosphocholine esterase activity and molecular function this GO :0005543 and phospholipid binding and molecular function this GO :0005576 and extracellular region and cellular component this GO :0005615 and extracellular space and cellular component this GO :0005737 and cytoplasm and cellular component this GO :0016042 and lipid catabolic process and biological process this GO :0034362 and low-density lipoprotein particle and cellular component this GO :0034374 and low-density lipoprotein particle remodeling and biological process this GO :0034440 and lipid oxidation and biological process this GO :0034441 and plasma lipoprotein particle oxidation and biological process this GO :0044267 and cellular protein metabolic process and biological process this GO :0047499 and calcium-independent phospholipase A2 activity and molecular function this GO :0050729 and positive regulation of inflammatory response and biological process this GO :0090026 and positive regulation of monocyte chemotaxis and biological process, this GO :0003847 : 1-alkyl-2-acetylglycerophosphocholine esterase activity, this GO :0003847 : 1-alkyl-2-acetylglycerophosphocholine esterase activity and also this GO :0005543 : phospholipid binding and also this GO :0047499 : calcium-independent phospholipase A2 activity, this GO :0005543 : phospholipid binding, this GO :0047499 : calcium-independent phospholipase A2 activity, phospholipase A2 |
| Identity: |
9040 |
| Gene: |
PLA2G7 |
More about : PLA2G7 |
| Long gene name: |
phospholipase A2 group VII |
| Synonyms gene name: |
group VII (platelet-activating factor acetylhydrolase, phospholipase A2, plasma) |
| Synonyms: |
PAFAH LDL-PLA2 |
| Locus: |
6p12, 3 |
| Discovery year: |
1999-09-20 |
| GenBank acession: |
U20157 |
| Entrez gene record: |
7941 |
| Pubmed identfication: |
7700381 8624782 |
| Classification: |
Phospholipases |
| Havana BLAST/BLAT: |
OTTHUMG00000014789 |