| Category: |
Antibody |
| Concentration: |
pH 7, with 0, 09% sodium azide, 4, In 1X PBS |
| Form: |
Antigen affinity purified |
| Conjugation: |
Unconjugated |
| Clone: |
Polyclonal antibody |
| Recognised antigen: |
Anaplastic lymphoma kinase (Alk) |
| Host animal: |
Rabbit (Oryctolagus cuniculus) |
| Clonality: |
Polyclonal (rabbit origin) |
| Species reactivity: |
Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur, Mouse (Mus musculus) |
| Tested applications: |
ELISA, WB |
| Recommended dilutions: |
Western blot: 1:2000 |
| Notes: |
Titration of the Alk antibody may be required due to differences in protocols and secondary/substrate sensitivity |
| Intented use: |
This Alk antibodyis to be used only for research purposes and not for diagnostics |
| Uniprot #: |
P97793 |
| Purity: |
Antigen affinity |
| Description: |
ALK induces tyrosine phosphorylation of CBL, Acts as a receptor for ligands pleiotrophin (PTN), Drives NF-kappa-B activation, FRS2, Following activation by ligand, IRS1 and SHC1, MDK-binding induces phosphorylation of the ALK target insulin receptor substrate (IRS1), PTN-binding induces MAPK pathway activation, Phosphorylates almost exclusively at the first tyrosine of the Y-x-x-x-Y-Y motif, Recruitment of IRS1 to activated ALK and the activation of NF-kappa-B are essential for the autocrine growth and survival signaling of MDK, Transduces signals from ligands at the cell surface, [UniProt], a PTN-related factor, a secreted growth factor, activates mitogen-activated protein kinases (MAPKs) and PI3-kinase, and midkine (MDK), as well as of the MAP kinases MAPK1/ERK2 and MAPK3/ERK1, probably through IRS1 and the activation of the AKT serine/threonine kinase, resulting also in cell proliferation induction, through specific activation of the mitogen-activated protein kinase (MAPK) pathway, thus participating in PTN and MDK signal transduction, which is important for the anti-apoptotic signaling of PTN and regulation of cell proliferation, Anaplastic lymphoma kinase is a neuronal orphan receptor tyrosine kinase that is essentially and transiently expressed in specific regions of the central and peripheral nervous systems and plays an important role in the genesis and differentiation of the nervous system |
| Immunogen: |
This Alk antibody was produced from a rabbit immunized with a KLH conjugated synthetic peptide between 1517-1550 amino acids mouse Alk |
| Storage: |
Celcius or lower, Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity, Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized, Aliquot the Alk antibody and store frozen at -20 deg |
| Properties: |
C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24° |
| Gene target: |
Anaplastic lymphoma kinase (Alk) |
| Short name: |
Anaplastic lymphoma kinase Antibody (Alk) |
| Technique: |
antibodies against human proteins, antibodies for, Antibody |
| Alternative name: |
Anaplastic lymphoma phosphorylation catalyst (Antibody to) (anaplastic lymphoma receptor tyrosine kinase) |
| Alternative technique: |
antibodies |