| Category: |
Antibody |
| Concentration: |
pH 7, with 0, 09% sodium azide, 4, In 1X PBS |
| Form: |
Antigen affinity purified |
| Conjugation: |
Unconjugated |
| Clone: |
Polyclonal antibody |
| Recognised antigen: |
Eed |
| Host animal: |
Rabbit (Oryctolagus cuniculus) |
| Clonality: |
Polyclonal (rabbit origin) |
| Species reactivity: |
Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur, Mouse (Mus musculus) |
| Tested applications: |
ELISA, WB |
| Recommended dilutions: |
Western blot: 1:1000 |
| Notes: |
Titration of the Eed antibody may be required due to differences in protocols and secondary/substrate sensitivity |
| Intented use: |
This Eed antibodyis to be used only for research purposes and not for diagnostics |
| Uniprot #: |
Q921E6 |
| Purity: |
Antigen affinity |
| Description: |
Also recognizes 'Lys-26' trimethylated histone H1 with the effect of inhibiting PRC2 complex methyltransferase activity on nucleosomal histone H3 'Lys-27', Component of the PRC2/EED- EZH2 complex, Genes repressed by the PRC2/EED-EZH2 complex include HOXA7, HOXB6 and HOXC8, May prevent transcriptional activation of facultative heterochromatin during differentiation, May regulate hippocampal synaptic plasticity in the developing brain, Plays a role in X chromosome inactivation (XCI), Required for development of secondary trophoblast giant cells during placental development, Required for stable maintenance of XCI in both embryonic and extraembryonic tissues, The PRC2/EED-EZH2 complex may also serve as a recruiting platform for DNA methyltransferases, enabling the propagation of this repressive mark (By similarity), in which one of the two X chromosomes in female mammals is transcriptionally silenced to equalize X-linked gene dosage with XY males, leading to transcriptional repression of the affected target gene, thereby linking two epigenetic repression systems (By similarity), whereas H3 'Lys-27' recognition has the opposite effect, which methylates 'Lys-9' and 'Lys-27' of histone H3, Polycomb group (PcG) protein |
| Immunogen: |
This Eed antibody was produced from a rabbit immunized with a KLH conjugated synthetic peptide between 40-74 amino acids from the N-terminal region of mouse Eed |
| Storage: |
Celcius or lower, Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity, Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized, Aliquot the Eed antibody and store frozen at -20 deg |
| Properties: |
C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24° |
| Gene target: |
Eed |
| Short name: |
Eed Antibody |
| Technique: |
antibodies against human proteins, antibodies for, Antibody |
| Alternative name: |
Eed (Antibody to) |
| Alternative technique: |
antibodies |
| Identity: |
3188 |
| Gene: |
EED |
More about : EED |
| Long gene name: |
embryonic ectoderm development |
| Synonyms: |
WAIT-1 HEED |
| Synonyms name: |
WD protein associating with integrin cytoplasmic tails 1 |
| Locus: |
11q14, 2 |
| Discovery year: |
1998-12-09 |
| GenBank acession: |
AF078933 |
| Entrez gene record: |
8726 |
| Pubmed identfication: |
9765275 9806832 |
| RefSeq identity: |
NM_003797 |
| Classification: |
Polycomb repressive complex 2 WD repeat domain containing |
| Havana BLAST/BLAT: |
OTTHUMG00000167209 |