| Category: |
Antibody |
| Concentration: |
09% sodium azide, 4 with 0, In 1X PBS pH 7 |
| Form: |
Purified |
| Conjugation: |
histidine |
| Clone: |
Polyclonal antibody |
| Recognised antigen: |
His Tag |
| Host animal: |
Rabbit (Oryctolagus cuniculus) |
| Clonality: |
Polyclonal (rabbit origin) |
| Species reactivity: |
Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur |
| Tested applications: |
ELISA, WB |
| Recommended dilutions: |
Western blot: 1:1000 |
| Notes: |
Titration of the anti-His Tag antibody may be required due to differences in protocols and secondary/substrate sensitivity |
| Intented use: |
This anti-His Tag antibodyis to be used only for research purposes and not for diagnostics |
| Purity: |
Purified |
| Description: |
6xHIS-tagged fusion proteins are easily purified from cell lysates by affinity chromatography using Nickel-Sepharose resin, Epitope tags consisting of short sequences recognized by well-characterized monoclonal antibodies have been widely used in the study of protein expression in various systems |
| Immunogen: |
HHHHHH peptide was used as the immunogen for the anti-His Tag antibody |
| Storage: |
Celcius or lower, Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity, Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized, Aliquot the anti-His Tag antibody and store frozen at -20 deg |
| Properties: |
C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24° |
| Gene target: |
His Tag |
| Short name: |
His Tag Antibody |
| Technique: |
Tags, antibodies against human proteins, antibodies for, Antibody |
| Label: |
His |
| Alternative name: |
histidine detection labelled (Antibody to) |
| Alternative technique: |
antibodies |