| Category: |
Antibody |
| Concentration: |
pH 7, with 0, 09% sodium azide, 4, In 1X PBS |
| Form: |
Purified |
| Conjugation: |
Unconjugated |
| Clone: |
Polyclonal antibody |
| Recognised antigen: |
CDK2 |
| Host animal: |
Rabbit (Oryctolagus cuniculus) |
| Clonality: |
Polyclonal (rabbit origin) |
| Species reactivity: |
Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur, Human (Homo sapiens) |
| Tested applications: |
ELISA, FACS, IHC, WB |
| Recommended dilutions: |
Flow Cytometry: 1:10-1:50, IHC (Paraffin): 1:50-1:100, Western blot: 1:1000 |
| Notes: |
Titration of the CDK2 antibody may be required due to differences in protocols and secondary/substrate sensitivity |
| Intented use: |
This CDK2 antibodyis to be used only for research purposes and not for diagnostics |
| Uniprot #: |
P24941 |
| Purity: |
Purified |
| Description: |
Activity of CDK2 is maximal during S phase and G2, Acts at the G1-S transition to promote the E2F transcriptional program and the initiation of DNA synthesis, B1, B3, BRCA2, CDK7, Crucial role in orchestrating a fine balance between cellular proliferation, D, EZH2, EZH2 phosphorylation promotes H3K27me3 maintenance and epigenetic gene silencing, Interacts with cyclins A, MYC, NPAT, NPM1, Phosphorylates CABLES1 (By similarity), Phosphorylates CTNNB1, RB1, Triggers duplication of centrosomes and DNA, USP37, [UniProt], activated by interaction with cyclin E during the early stages of DNA synthesis to permit G1-S transition, and DNA repair in human embryonic stem cells (hESCs), and coordinates the activation of cyclin B/CDK1 at the centrosome and in the nucleus, and modulates G2 progression, and subsequently activated by cyclin A2 (cyclin A1 in germ cells) during the late stages of DNA replication to drive the transition from S phase to mitosis, but dispensable for mitosis, cell death, controls the timing of entry into mitosis/meiosis by controlling the subsequent activation of cyclin B/CDK1 by phosphorylation, essential for meiosis, or E, p53/TP53, the G2 phase, CDK2 is a serine/threonine-protein kinase involved in the control of the cell cycle |
| Immunogen: |
A portion of amino acids 230-260 from the human protein was used as the immunogen for this CDK2 antibody |
| Storage: |
Celcius or lower, Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity, Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized, Aliquot the CDK2 antibody and store frozen at -20 deg |
| Properties: |
C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24° |
| Gene target: |
CDK2 |
| Short name: |
CDK2 Antibody |
| Technique: |
antibodies against human proteins, antibodies for, Antibody |
| Alternative name: |
CDK2 (Antibody to) |
| Alternative technique: |
antibodies |
| Identity: |
1771 |
| Gene: |
CDK2 |
More about : CDK2 |
| Long gene name: |
cyclin dependent kinase 2 |
| Locus: |
12q13, 2 |
| Discovery year: |
1992-02-28 |
| GenBank acession: |
M68520 |
| Entrez gene record: |
1017 |
| Pubmed identfication: |
1717994 8275715 |
| Classification: |
Cyclin dependent kinases |
| Havana BLAST/BLAT: |
OTTHUMG00000170575 |