| Category: |
Antibody |
| Concentration: |
pH 7, with 0, 09% sodium azide, 4, In 1X PBS |
| Form: |
Purified |
| Conjugation: |
Unconjugated |
| Clone: |
Polyclonal antibody |
| Recognised antigen: |
HSP60 |
| Host animal: |
Rabbit (Oryctolagus cuniculus) |
| Clonality: |
Polyclonal (rabbit origin) |
| Species reactivity: |
Due to limited knowledge and inability to test the antibody against all known species, Mouse (Mus musculus) , we cannot guarantee that no other cross reactivity can occur, Human (Homo sapiens) |
| Tested applications: |
ELISA, IHC, WB |
| Recommended dilutions: |
IHC (Paraffin): 1:50-1:100, Western blot: 1:1000 |
| Notes: |
Titration of the HSP60 antibody may be required due to differences in protocols and secondary/substrate sensitivity |
| Intented use: |
This HSP60 antibodyis to be used only for research purposes and not for diagnostics |
| Uniprot #: |
Q0VDF9 |
| Purity: |
Purified |
| Description: |
Consistent with their functions as chaperones, Data demonstrates that recombinant Hsp60 and Hsp10 have been shown to accelerate the activation of procaspase 3 by cytochrome c and dATP in an ATP dependent manner, GroEL, GroES, Hsp10 were expressed in a E, Hsp60 and Hsp10 have been suggested to act as docking molecules with a passive role in the maturation of caspase processing, Hsp60 is localized in the mitochondrial matrix and in plants Hsp60 is localized in the chloroplast, Hsp60 with its co chaperonin, Hsps are intracellular proteins which are thought to serve protective functions against infection and cellular stress, In eukaryotes, Mitochondria, These similarities are supported by recent studies where the single ring human mitochondrial homolog, This study has demonstrated that expression of Hsp60-Hsp10 was able to carry out all essential in vivo functions of GroEL and its co chaperonin, artherosclerosis and chlamydial disease, chloroplasts and bacteria have a common ancestry (>, coli strain, engineered so that the groE operon is under strict regulatory control, however several recent studies indicate that members of the Hsp60 family are linked to a number of autoimmune diseases, ii) induction with environmental stress such as heat shock, iii) homo oligomeric structures of either 7 or 14 subunits which reversibly dissociate in the presence of magnesium ions and ATP, iv) ATPase activity and v) a role in folding and assembly of oligomeric protein structures, the E, 1 billion years) and this fact together with the high degree of homology between the divegent Hsp60s would indicate that these proteins carry out a primitive but important function which is similar to all of these different species, Hsp60 is a member of a highly conserved family which includes molecular chaperones from several species such as plant Hsp60 (known as Rubisco binding protein), The common characteristics of the Hsp60s from the divergent species are i) high abundance, coli Hsp60 and 65 kDa major antigen of mycobacteria |
| Immunogen: |
A portion of amino acids 80-109 from the human protein was used as the immunogen for this HSP60 antibody |
| Storage: |
Celcius or lower, Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity, Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized, Aliquot the HSP60 antibody and store frozen at -20 deg |
| Properties: |
C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24° |
| Gene target: |
HSP60 |
| Short name: |
HSP60 Antibody |
| Technique: |
antibodies against human proteins, antibodies for, Antibody |
| Alternative name: |
HSP60 (Antibody to) |
| Alternative technique: |
antibodies |
| Identity: |
5261 |
| Gene: |
HSPD1 |
More about : HSPD1 |
| Long gene name: |
heat shock protein family D (Hsp60) member 1 |
| Synonyms gene: |
SPG13 |
| Synonyms gene name: |
heat shock 60kD protein 1 (chaperonin) spastic paraplegia 13 (autosomal dominant) heat shock 60kDa protein 1 (chaperonin) |
| Synonyms: |
GroEL HSP60 |
| Locus: |
2q33, 1 |
| Discovery year: |
1991-07-19 |
| GenBank acession: |
M34664 |
| Entrez gene record: |
3329 |
| Pubmed identfication: |
1980192 11898127 |
| RefSeq identity: |
NM_002156 |
| Classification: |
Chaperonins |
| Havana BLAST/BLAT: |
OTTHUMG00000154463 |