| Category: |
Antibody |
| Concentration: |
pH 7, with 0, 09% sodium azide, 4, In 1X PBS |
| Form: |
Purified |
| Conjugation: |
Unconjugated |
| Clone: |
Polyclonal antibody |
| Recognised antigen: |
VHL |
| Host animal: |
Rabbit (Oryctolagus cuniculus) |
| Clonality: |
Polyclonal (rabbit origin) |
| Species reactivity: |
Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur, Human (Homo sapiens) |
| Tested applications: |
ELISA, FACS, WB, IHC |
| Recommended dilutions: |
Flow Cytometry: 1:10-1:50, IHC (Paraffin): 1:50-1:100, Western blot: 1:1000 |
| Notes: |
Titration of the VHL antibody may be required due to differences in protocols and secondary/substrate sensitivity |
| Intented use: |
This VHL antibodyis to be used only for research purposes and not for diagnostics |
| Uniprot #: |
P40337 |
| Purity: |
Purified |
| Description: |
A germline mutation of VHL gene is the basis of familial inheritance of VHL syndrome, RNA polymerase II subunit POLR2G/RPB7 is also reported to be a target of this protein, The protein is a component of the protein complex that includes elongin B, This protein is involved in the ubiquitination and degradation of hypoxia-inducible-factor (HIF), and cullin-2, and possesses ubiquitin ligase E3 activity, elongin C, which is a transcription factor that plays a central role in the regulation of gene expression by oxygen, Von Hippel-Lindau syndrome (VHL) is a dominantly inherited familial cancer syndrome predisposing to a variety of malignant and benign tumors |
| Immunogen: |
A portion of amino acids 181-210 from the human protein was used as the immunogen for this VHL antibody |
| Storage: |
Celcius or lower, Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity, Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized, Aliquot the VHL antibody and store frozen at -20 deg |
| Properties: |
C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24° |
| Gene target: |
VHL |
| Short name: |
VHL Antibody |
| Technique: |
antibodies against human proteins, antibodies for, Antibody |
| Alternative name: |
E3 ubiquitin protein ligase (Antibody to), von Hippel-Lindau tumor suppressor |
| Alternative technique: |
antibodies |
| Alternative to gene target: |
BT, DNA-templated and biological process this GO :0006508 and proteolysis and biological process this GO :0006950 and response to stress and biological process this GO :0008134 and transcription factor binding and molecular function this GO :0008285 and negative regulation of cell proliferation and biological process this GO :0016020 and membrane and cellular component this GO :0016567 and protein ubiquitination and biological process this GO :0019899 and enzyme binding and molecular function this GO :0043066 and negative regulation of apoptotic process and biological process this GO :0045111 and intermediate filament cytoskeleton and cellular component this GO :0045597 and positive regulation of cell differentiation and biological process this GO :0045893 and positive regulation of transcription, DNA-templated and biological process this GO :0050821 and protein stabilization and biological process this GO :0061418 and regulation of transcription from RNA polymerase II promoter in response to hypoxia and biological process this GO :0061428 and negative regulation of transcription from RNA polymerase II promoter in response to hypoxia and biological process this GO :0071456 and cellular response to hypoxia and biological process, E3 ubiquitin protein ligase, HRCA1 and pVHL and RCA1 and VHL1, VHL and IDBG-17819 and ENSG00000134086 and 7428, Vhl and IDBG-178105 and ENSMUSG00000033933 and 22346, enzyme binding, nuclei, this GO :0000122 and negative regulation of transcription from RNA polymerase II promoter and biological process this GO :0000902 and cell morphogenesis and biological process this GO :0004842 and ubiquitin-protein transferase activity and molecular function this GO :0005515 and protein binding and molecular function this GO :0005634 and nucleus and cellular component this GO :0005654 and nucleoplasm and cellular component this GO :0005739 and mitochondrion and cellular component this GO :0005783 and endoplasmic reticulum and cellular component this GO :0005829 and cytosol and cellular component this GO :0006355 and regulation of transcription, this GO :0004842 : ubiquitin-protein transferase activity, this GO :0004842 : ubiquitin-protein transferase activity and also this GO :0005515 : protein binding and also this GO :0008134 : transcription factor binding and also this GO :0019899 : enzyme binding, this GO :0005515 : protein binding, this GO :0008134 : transcription factor binding, this GO :0019899 : enzyme binding, 79817 and IDBG-644624 and ENSBTAG00000016101 and 540957, von Hippel-Lindau tumor suppressor |
| Identity: |
12687 |
| Gene: |
VHL |
More about : VHL |
| Long gene name: |
von Hippel-Lindau tumor suppressor |
| Synonyms gene name: |
E3 ubiquitin protein ligase , von Hippel-Lindau syndrome von Hippel-Lindau tumor suppressor |
| Synonyms: |
VHL1 |
| Locus: |
3p25, 3 |
| Discovery year: |
1986-01-01 |
| GenBank acession: |
L15409 |
| Entrez gene record: |
7428 |
| Pubmed identfication: |
9671762 |
| RefSeq identity: |
NM_000551 |
| Havana BLAST/BLAT: |
OTTHUMG00000128668 |
| Locus Specific Databases: |
UMD Locus Specific Databases LRG_322 |