| Category: |
Antibody |
| Concentration: |
pH 7, with 0, 09% sodium azide, 4, In 1X PBS |
| Form: |
Antigen affinity purified |
| Conjugation: |
Unconjugated |
| Clone: |
Polyclonal antibody |
| Recognised antigen: |
ARF1 |
| Host animal: |
Rabbit (Oryctolagus cuniculus) |
| Clonality: |
Polyclonal (rabbit origin) |
| Species reactivity: |
Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur, Human (Homo sapiens) |
| Tested applications: |
ELISA, IHC, WB |
| Recommended dilutions: |
IHC (Paraffin): 1:50-1:100, Western blot: 1:1000 |
| Notes: |
Titration of the ARF1 antibody may be required due to differences in protocols and secondary/substrate sensitivity |
| Intented use: |
This ARF1 antibodyis to be used only for research purposes and not for diagnostics |
| Uniprot #: |
P84077 |
| Purity: |
Antigen affinity |
| Description: |
Deactivation induces the redistribution of the entire Golgi complex to the endoplasmic reticulum, In its GTP-bound form, Involved in protein trafficking among different compartments, Modulates vesicle budding and uncoating within the Golgi complex, The GTP-bound form interacts with PICK1 to limit PICK1-mediated inhibition of Arp2/3 complex activity, The hydrolysis of ARF1-bound GTP, [UniProt], an ADP-ribosyltransferase, is required for dissociation of coat proteins from Golgi membranes and vesicles, its triggers the association with coat proteins with the Golgi membrane, regulation of synaptic plasicity of excitatory synapses and spine shrinkage during long-term depression (LTD), suggesting a crucial role in protein trafficking, the function is linked to AMPA receptor (AMPAR) trafficking, which is mediated by ARFGAPs proteins, ARF1 is a GTP-binding protein that functions as an allosteric activator of the cholera toxin catalytic subunit |
| Immunogen: |
A portion of amino acids 80-106 from the human protein was used as the immunogen for this ARF1 antibody |
| Storage: |
Celcius or lower, Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity, Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized, Aliquot the ARF1 antibody and store frozen at -20 deg |
| Properties: |
C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24° |
| Gene target: |
ARF1 |
| Short name: |
ARF1 Antibody |
| Technique: |
antibodies against human proteins, antibodies for, Antibody |
| Alternative name: |
ARF1 (Antibody to) |
| Alternative technique: |
antibodies |
| Identity: |
652 |
| Gene: |
ARF1 |
More about : ARF1 |
| Long gene name: |
ADP ribosylation factor 1 |
| Locus: |
1q42, 13 |
| Discovery year: |
1992-07-09 |
| GenBank acession: |
M84326 |
| Entrez gene record: |
375 |
| Pubmed identfication: |
1577740 |
| RefSeq identity: |
NM_001024227 |
| Classification: |
ARF GTPase family Endogenous ligands |
| Havana BLAST/BLAT: |
OTTHUMG00000037595 |