| Category: |
Antibody |
| Concentration: |
pH 7, with 0, 09% sodium azide, 4, In 1X PBS |
| Form: |
Antigen affinity purified |
| Conjugation: |
Unconjugated |
| Clone: |
Polyclonal antibody |
| Recognised antigen: |
Retinoblastoma / Rb (phospho-S249) |
| Host animal: |
Rabbit (Oryctolagus cuniculus) |
| Clonality: |
Polyclonal (rabbit origin) |
| Species reactivity: |
Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur, Human (Homo sapiens) |
| Tested applications: |
ELISA, IHC, WB |
| Recommended dilutions: |
IHC (Paraffin): 1:50-1:100, Western blot: 1:1000 |
| Notes: |
Titration of the p-Rb antibody may be required due to differences in protocols and secondary/substrate sensitivity |
| Intented use: |
This phospho-p-Rb antibodyis to be used only for research purposes and not for diagnostics |
| Uniprot #: |
P06400 |
| Purity: |
Antigen affinity |
| Description: |
Defects in RB1 are also a cause of bladder cancer and osteogenic sarcoma, Defects in RB1 are the cause of childhood cancer retinoblastoma (RB), It forms a complex with adenovirus E1A and with SV40 large T antigen, RB1 is a potent inhibitor of E2F-mediated trans-activation, This protein inhibits the intrinsic kinase activity of TAF1, a congenital malignant tumor that arises from the nuclear layers of the retina, acts as a tumor suppressor, and also recruits and targets histone methyltransferase SUV39H1 leading to epigenetic transcriptional repression, and may bind and modulate functionally certain cellular proteins with which T and E1A compete for pocket binding, RB1 likely acts as a regulator of other genes |
| Immunogen: |
This p-Rb antibody was produced from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding pS249 of human Retinoblastoma |
| Storage: |
Celcius or lower, Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity, Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized, Aliquot the p-Rb antibody and store frozen at -20 deg |
| Properties: |
C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24° |
| Gene target: |
Retinoblastoma / Rb (phospho-S249) |
| Short name: |
Retinoblastoma / Rb Antibody (phospho-S249) |
| Technique: |
antibodies against human proteins, antibodies for, Antibody |
| Alternative name: |
Retinoblastoma / Rb (Antibody to) (phosphorilated-S249) |
| Alternative technique: |
antibodies |