| Category: |
Antibody |
| Concentration: |
pH 7, with 0, 09% sodium azide, 4, In 1X PBS |
| Form: |
Purified |
| Conjugation: |
Unconjugated |
| Clone: |
Polyclonal antibody |
| Recognised antigen: |
Anti-PCNA |
| Host animal: |
Rabbit (Oryctolagus cuniculus) |
| Clonality: |
Polyclonal (rabbit origin) |
| Species reactivity: |
Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur, Human (Homo sapiens) |
| Tested applications: |
ELISA, FACS, IF, IHC, WB |
| Recommended dilutions: |
Flow Cytometry: 1:10-1:50, IHC (Paraffin): 1:10-1:50, Immunofluorescence: 1:10-1:50, Western blot: 1:1000 |
| Notes: |
Titration of the anti-PCNA antibody may be required due to differences in protocols and secondary/substrate sensitivity |
| Intented use: |
This anti-PCNA antibodyis to be used only for research purposes and not for diagnostics |
| Uniprot #: |
P12004 |
| Purity: |
Purified |
| Description: |
In response to DNA damage, This protein acts as a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication, this protein is ubiquitinated and is involved in the RAD6-dependent DNA repair pathway, PCNA is found in the nucleus and is a cofactor of DNA polymerase delta |
| Immunogen: |
A portion of amino acids 89-117 from the human protein was used as the immunogen for this anti-PCNA antibody |
| Storage: |
Celcius or lower, Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity, Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized, Aliquot the anti-PCNA antibody and store frozen at -20 deg |
| Properties: |
C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24° |
| Gene target: |
PCNA |
| Short name: |
Anti-PCNA Antibody |
| Technique: |
antibodies against human proteins, antibodies for, Antibody |
| Alternative name: |
antibody to-PCNA (Antibody to) |
| Alternative technique: |
antibodies |
| Identity: |
8729 |
| Gene: |
PCNA |
More about : PCNA |
| Long gene name: |
proliferating cell nuclear antigen |
| Locus: |
20p12, 3 |
| Discovery year: |
1990-01-15 |
| GenBank acession: |
J04718 |
| Entrez gene record: |
5111 |
| Pubmed identfication: |
2565339 |
| Havana BLAST/BLAT: |
OTTHUMG00000031798 |