| Category: |
Antibody |
| Concentration: |
pH 7, with 0, 09% sodium azide, 4, In 1X PBS |
| Form: |
Purified |
| Conjugation: |
Unconjugated |
| Clone: |
Polyclonal antibody |
| Recognised antigen: |
PHB |
| Host animal: |
Rabbit (Oryctolagus cuniculus) |
| Clonality: |
Polyclonal (rabbit origin) |
| Species reactivity: |
Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur, Human (Homo sapiens) |
| Tested applications: |
ELISA, FACS, IF, WB, IHC |
| Recommended dilutions: |
Flow Cytometry: 1:10-1:50, IHC (Paraffin): 1:10-1:50, Immunofluorescence: 1:10-1:50, Western blot: 1:1000 |
| Notes: |
Titration of the PHB antibody may be required due to differences in protocols and secondary/substrate sensitivity |
| Intented use: |
This PHB antibodyis to be used only for research purposes and not for diagnostics |
| Uniprot #: |
P35232 |
| Purity: |
Purified |
| Description: |
It is thought to be a negative regulator of cell proliferation and may be a tumor suppressor, Mutations have been linked to sporadic breast cancer, Prohibitin is expressed as two transcripts with varying lengths of 3' untranslated region, Prohibitin is an evolutionarily conserved protein that is ubiquitously expressed |
| Immunogen: |
A portion of amino acids 89-117 from the human protein was used as the immunogen for this PHB antibody |
| Storage: |
Celcius or lower, Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity, Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized, Aliquot the PHB antibody and store frozen at -20 deg |
| Properties: |
C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24° |
| Gene target: |
PHB |
| Short name: |
PHB Antibody |
| Technique: |
antibodies against human proteins, antibodies for, Antibody |
| Alternative name: |
prohibitin (Antibody to) |
| Alternative technique: |
antibodies |
| Alternative to gene target: |
DNA-templated and biological process this GO :0006366 and transcription from RNA polymerase II promoter and biological process this GO :0007165 and signal transduction and biological process this GO :0008285 and negative regulation of cell proliferation and biological process this GO :0010944 and negative regulation of transcription by competitive promoter binding and biological process this GO :0016020 and membrane and cellular component this GO :0016575 and histone deacetylation and biological process this GO :0019899 and enzyme binding and molecular function this GO :0030308 and negative regulation of cell growth and biological process this GO :0042826 and histone deacetylase binding and molecular function this GO :0042981 and regulation of apoptotic process and biological process this GO :0044212 and transcription regulatory region DNA binding and molecular function this GO :0045892 and negative regulation of transcription, DNA-templated and biological process this GO :0045893 and positive regulation of transcription, DNA-templated and biological process this GO :0050847 and progesterone receptor signaling pathway and biological process this GO :0060766 and negative regulation of androgen receptor signaling pathway and biological process this GO :0070062 and extracellular vesicular exosome and cellular component this GO :0071354 and cellular response to interleukin-6 and biological process this GO :0071897 and DNA biosynthetic process and biological process this GO :2000323 and negative regulation of glucocorticoid receptor signaling pathway and biological process, HEL-215 and HEL-S-54e and PHB1, PHB and IDBG-57598 and ENSG00000167085 and 5245, PHB and IDBG-638810 and ENSBTAG00000017120 and 530409, Phb and IDBG-209638 and ENSMUSG00000038845 and 18673, nuclei, this GO :0000122 and negative regulation of transcription from RNA polymerase II promoter and biological process this GO :0000981 and sequence-specific DNA binding RNA polymerase II transcription factor activity and molecular function this GO :0001649 and osteoblast differentiation and biological process this GO :0005515 and protein binding and molecular function this GO :0005634 and nucleus and cellular component this GO :0005654 and nucleoplasm and cellular component this GO :0005737 and cytoplasm and cellular component this GO :0005739 and mitochondrion and cellular component this GO :0005743 and mitochondrial inner membrane and cellular component this GO :0005887 and integral component of plasma membrane and cellular component this GO :0006260 and DNA replication and biological process this GO :0006355 and regulation of transcription, this GO :0000981 : sequence-specific DNA binding RNA polymerase II transcription factor activity, this GO :0000981 : sequence-specific DNA binding RNA polymerase II transcription factor activity and also this GO :0005515 : protein binding and also this GO :0019899 : enzyme binding and also this GO :0042826 : histone deacetylase binding and also this GO :0044212 : transcription regulatory region DNA binding, this GO :0005515 : protein binding, this GO :0019899 : enzyme binding, this GO :0042826 : histone deacetylase binding, this GO :0044212 : transcription regulatory region DNA binding, transcription regulatory region DNA binding, 237880, prohibitin |
| Identity: |
8912 |
| Gene: |
PHB |
More about : PHB |
| Long gene name: |
prohibitin |
| Synonyms: |
PHB1 |
| Locus: |
17q21, 33 |
| Discovery year: |
1991-03-04 |
| Entrez gene record: |
5245 |
| Pubmed identfication: |
10376528 8244394 |
| RefSeq identity: |
NM_002634 |
| Havana BLAST/BLAT: |
OTTHUMG00000134271 |