| Category: |
Antibody |
| Concentration: |
pH 7, with 0, 09% sodium azide, 4, In 1X PBS |
| Form: |
Antigen affinity purified |
| Conjugation: |
Unconjugated |
| Clone: |
Polyclonal antibody |
| Recognised antigen: |
AKT1S1 |
| Host animal: |
Rabbit (Oryctolagus cuniculus) |
| Clonality: |
Polyclonal (rabbit origin) |
| Species reactivity: |
Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur, Human (Homo sapiens) |
| Tested applications: |
ELISA, WB, IHC |
| Recommended dilutions: |
IHC (Paraffin): 1:10-1:50, Western blot: 1:1000 |
| Notes: |
Titration of the AKT1S1 antibody may be required due to differences in protocols and secondary/substrate sensitivity |
| Intented use: |
This AKT1S1 antibodyis to be used only for research purposes and not for diagnostics |
| Uniprot #: |
Q96B36 |
| Purity: |
Antigen affinity |
| Description: |
AKT1S1 negatively regulates mTOR activity in a manner that is dependent on its phosphorylation state and binding to 14-3-3 proteins, Activated mTORC1 up-regulates protein synthesis by phosphorylating key regulators of mRNA translation and ribosome synthesis, Amino acid-signaling to mTORC1 requires its relocalization to the lysosomes mediated by the Ragulator complex and the Rag GTPases, Growth factor-stimulated mTORC1 activation involves a AKT1-mediated phosphorylation of TSC1-TSC2, Inhibits RHEB-GTP-dependent mTORC1 activation, May also play a role in nerve growth factor-mediated neuroprotection, Substrate for AKT1 phosphorylation, Within mTORC1, [UniProt], but can also be activated by AKT1-independent mechanisms, mTORC1 is activated in response to growth factors or amino acids, mTORC1 phosphorylates EIF4EBP1 and releases it from inhibiting the elongation initiation factor 4E (eiF4E), mTORC1 phosphorylates and activates S6K1 at 'Thr-389', which leads to the activation of the RHEB GTPase that potently activates the protein kinase activity of mTORC1, which regulates cell growth and survival in response to nutrient and hormonal signals, which then promotes protein synthesis by phosphorylating PDCD4 and targeting it for degradation, Subunit of mTORC1 |
| Immunogen: |
A portion of amino acids 227-256 from the human protein was used as the immunogen for this AKT1S1 antibody |
| Storage: |
Celcius or lower, Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity, Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized, Aliquot the AKT1S1 antibody and store frozen at -20 deg |
| Properties: |
C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24° |
| Gene target: |
AKT1S1 |
| Short name: |
AKT1S1 Antibody |
| Technique: |
antibodies against human proteins, antibodies for, Antibody |
| Alternative name: |
AKT1S1 (Antibody to) |
| Alternative technique: |
antibodies |
| Identity: |
28426 |
| Gene: |
AKT1S1 |
More about : AKT1S1 |
| Long gene name: |
AKT1 substrate 1 |
| Synonyms gene name: |
AKT1 substrate 1 (proline-rich) |
| Synonyms: |
PRAS40 MGC2865 Lobe |
| Synonyms name: |
40 kDa , proline-rich Akt substrate |
| Locus: |
19q13, 33 |
| Discovery year: |
2004-03-04 |
| GenBank acession: |
BC022241 |
| Entrez gene record: |
84335 |
| Pubmed identfication: |
12524439 |
| RefSeq identity: |
NM_032375 |
| Classification: |
MTOR complex 1 |
| Havana BLAST/BLAT: |
OTTHUMG00000150246 |