| Category: |
Antibody |
| Concentration: |
pH 7, with 0, 09% sodium azide, 4, In 1X PBS |
| Form: |
Antigen affinity purified |
| Conjugation: |
Unconjugated |
| Clone: |
Polyclonal antibody |
| Recognised antigen: |
MeCP2 |
| Host animal: |
Rabbit (Oryctolagus cuniculus) |
| Clonality: |
Polyclonal (rabbit origin) |
| Species reactivity: |
Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur, Human (Homo sapiens) |
| Tested applications: |
ELISA, FACS, IHC, WB |
| Recommended dilutions: |
Flow Cytometry: 1:10-1:50, IHC (Paraffin): 1:50-1:100, Western blot: 1:1000 |
| Notes: |
Titration of the MeCP2 antibody may be required due to differences in protocols and secondary/substrate sensitivity |
| Intented use: |
This MeCP2 antibodyis to be used only for research purposes and not for diagnostics |
| Uniprot #: |
P51608 |
| Purity: |
Antigen affinity |
| Description: |
Binds both 5-methylcytosine (5mC) and 5-hydroxymethylcytosine (5hmC)-containing DNA, It can bind specifically to a single methyl-CpG pair, It is not influenced by sequences flanking the methyl-CpGs, Mediates transcriptional repression through interaction with histone deacetylase and the corepressor SIN3A, [UniProt], with a preference for 5-methylcytosine (5mC), MeCP2 is a chromosomal protein that binds to methylated DNA |
| Immunogen: |
A portion of amino acids 58-87 from the human protein was used as the immunogen for this MeCP2 antibody |
| Storage: |
Celcius or lower, Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity, Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized, Aliquot the MeCP2 antibody and store frozen at -20 deg |
| Properties: |
C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24° |
| Gene target: |
MeCP2 |
| Short name: |
MeCP2 Antibody |
| Technique: |
antibodies against human proteins, antibodies for, Antibody |
| Alternative name: |
MeCP2 (Antibody to) |
| Alternative technique: |
antibodies |
| Identity: |
10470 |
| Gene: |
RTT |
More about : RTT |
| Long gene name: |
methyl-CpG binding protein 2 |
| Synonyms gene: |
MRX16 MRX79 |
| Synonyms gene name: |
X-linked 16 mental retardation, X-linked 79 Rett syndrome methyl CpG binding protein 2 (Rett syndrome) methyl CpG binding protein 2 , mental retardation |
| Synonyms: |
MECP2 |
| Locus: |
Xq28 |
| Discovery year: |
1996-09-03 |
| GenBank acession: |
AF158180 |
| Entrez gene record: |
4204 |
| Pubmed identfication: |
1606614 10508514 |
| RefSeq identity: |
NM_004992 |
| Classification: |
X-linked mental retardation Methyl-CpG binding domain containing |
| Havana BLAST/BLAT: |
OTTHUMG00000024229 |
| Locus Specific Databases: |
RettBASE Mental Retardation database LRG_764 |