| Category: |
Antibody |
| Concentration: |
pH 7, with 0, 09% sodium azide, 4, In 1X PBS |
| Form: |
Antigen affinity purified |
| Conjugation: |
Unconjugated |
| Clone: |
Polyclonal antibody |
| Recognised antigen: |
ATG12 |
| Host animal: |
Rabbit (Oryctolagus cuniculus) |
| Clonality: |
Polyclonal (rabbit origin) |
| Species reactivity: |
Due to limited knowledge and inability to test the antibody against all known species, Mouse (Mus musculus) , we cannot guarantee that no other cross reactivity can occur, Human (Homo sapiens) |
| Tested applications: |
ELISA, IF, WB |
| Recommended dilutions: |
Immunofluorescence: 1:50-1:100, Western blot: 1:1000 |
| Notes: |
Titration of the ATG12 antibody may be required due to differences in protocols and secondary/substrate sensitivity |
| Intented use: |
This ATG12 antibodyis to be used only for research purposes and not for diagnostics |
| Uniprot #: |
O94817 |
| Purity: |
Antigen affinity |
| Description: |
Macroautophagy involves the formation of double-membrane bound autophagosomes which enclose the cytoplasmic constituent targeted for degradation in a membrane bound structure, a ubiquitin-activating enzyme E1-like protein essential for the conjugation system that mediates membrane fusion in autophagy, it is also responsible for the degradation of active cytoplasmic enzymes and organelles during nutrient starvation, which then fuse with the lysosome (or vacuole) releasing a single-membrane bound autophagic bodies which are then degraded within the lysosome (or vacuole), <, APG12L/ATG12 is the human homolog of yeast APG12, Macroautophagy is the major inducible pathway for the general turnover of cytoplasmic constituents in eukaryotic cells, p> |
| Immunogen: |
A portion of amino acids 1-50 from the human protein was used as the immunogen for this ATG12 antibody |
| Storage: |
Celcius or lower, Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity, Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized, Aliquot the ATG12 antibody and store frozen at -20 deg |
| Properties: |
C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24° |
| Gene target: |
ATG12 |
| Short name: |
ATG12 Antibody |
| Technique: |
antibodies against human proteins, antibodies for, Antibody |
| Alternative name: |
ATG12 (Antibody to) |
| Alternative technique: |
antibodies |
| Identity: |
588 |
| Gene: |
ATG12 |
More about : ATG12 |
| Long gene name: |
autophagy related 12 |
| Synonyms gene: |
APG12L |
| Synonyms gene name: |
Apg12 (autophagy 12, S, cerevisiae) , cerevisiae) ATG12 autophagy related 12 homolog (S, cerevisiae)-like APG12 autophagy 12-like (S |
| Synonyms: |
APG12 |
| Synonyms name: |
APG12 autophagy 12-like |
| Locus: |
5q22, 3 |
| Discovery year: |
1999-10-01 |
| GenBank acession: |
AB017507 |
| Entrez gene record: |
9140 |
| Pubmed identfication: |
9852036 |
| RefSeq identity: |
NM_004707 |
| Classification: |
Autophagy related |
| Havana BLAST/BLAT: |
OTTHUMG00000128889 |