| Category: |
Antibody |
| Concentration: |
pH 7, with 0, 09% sodium azide, 4, In 1X PBS |
| Form: |
Purified |
| Conjugation: |
Unconjugated |
| Clone: |
Polyclonal antibody |
| Recognised antigen: |
SPHK1 |
| Host animal: |
Rabbit (Oryctolagus cuniculus) |
| Clonality: |
Polyclonal (rabbit origin) |
| Species reactivity: |
Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur, Human (Homo sapiens) |
| Tested applications: |
ELISA, FACS, IHC, WB |
| Recommended dilutions: |
Flow Cytometry: 1:10-1:50, IHC (Paraffin): 1:50-1:100, Western blot: 1:1000 |
| Notes: |
Titration of the SPHK1 antibody may be required due to differences in protocols and secondary/substrate sensitivity |
| Intented use: |
This SPHK1 antibodyis to be used only for research purposes and not for diagnostics |
| Uniprot #: |
Q9NYA1 |
| Purity: |
Purified |
| Description: |
Competitive inhibitors of SPHK block formation of SPP and selectively inhibit cellular proliferation induced by a variety of factors, Intracellularly, MIM 173430) and serum, Various stimuli increase cellular levels of SPP by activation of sphingosine kinase (SPHK), and extracellularly, including platelet-derived growth factor (e, it is a ligand for EDG1 (MIM 601974), it regulates proliferation and survival, the enzyme that catalyzes the phosphorylation of sphingosine, SPHK1 is a novel lipid messenger with both intracellular and extracellular functions, g |
| Immunogen: |
A portion of amino acids 313-343 from the human protein was used as the immunogen for this SPHK1 antibody |
| Storage: |
Celcius or lower, Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity, Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized, Aliquot the SPHK1 antibody and store frozen at -20 deg |
| Properties: |
C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24° |
| Gene target: |
SPHK1 |
| Short name: |
SPHK1 Antibody |
| Technique: |
antibodies against human proteins, antibodies for, Antibody |
| Alternative name: |
sphin this GO sine kinase 1 (Antibody to) |
| Alternative technique: |
antibodies |
| Alternative to gene target: |
SPHK, SPHK1 and IDBG-643068 and ENSBTAG00000008507 and 618605, SPHK1 and IDBG-69650 and ENSG00000176170 and 8877, Sphk1 and IDBG-214470 and ENSMUSG00000061878 and 20698, nuclei, protein phosphatase 2A binding, this GO :0000287 and magnesium ion binding and molecular function this GO :0001568 and blood vessel development and biological process this GO :0001934 and positive regulation of protein phosphorylation and biological process this GO :0001956 and positive regulation of neurotransmitter secretion and biological process this GO :0003677 and DNA binding and molecular function this GO :0003951 and NAD+ kinase activity and molecular function this GO :0004143 and diacylglycerol kinase activity and molecular function this GO :0005515 and protein binding and molecular function this GO :0005516 and calmodulin binding and molecular function this GO :0005524 and ATP binding and molecular function this GO :0005634 and nucleus and cellular component this GO :0005737 and cytoplasm and cellular component this GO :0005829 and cytosol and cellular component this GO :0005886 and plasma membrane and cellular component this GO :0006457 and protein folding and biological process this GO :0006665 and sphin this GO lipid metabolic process and biological process this GO :0006670 and sphin this GO sine metabolic process and biological process this GO :0006954 and inflammatory response and biological process this GO :0007165 and signal transduction and biological process this GO :0007205 and protein kinase C-activating G-protein coupled receptor signaling pathway and biological process this GO :0007420 and brain development and biological process this GO :0007565 and female pregnancy and biological process this GO :0008021 and synaptic vesicle and cellular component this GO :0008284 and positive regulation of cell proliferation and biological process this GO :0008481 and sphinganine kinase activity and molecular function this GO :0009267 and cellular response to starvation and biological process this GO :0010803 and regulation of tumor necrosis factor-mediated signaling pathway and biological process this GO :0010976 and positive regulation of neuron projection development and biological process this GO :0014070 and response to organic cyclic compound and biological process this GO :0014075 and response to amine and biological process this GO :0016310 and phosphorylation and biological process this GO :0017050 and D-erythro-sphin this GO sine kinase activity and molecular function this GO :0019371 and cyclooxygenase pathway and biological process this GO :0019722 and calcium-mediated signaling and biological process this GO :0030148 and sphin this GO lipid biosynthetic process and biological process this GO :0030307 and positive regulation of cell growth and biological process this GO :0030335 and positive regulation of cell migration and biological process this GO :0030424 and axon and cellular component this GO :0031398 and positive regulation of protein ubiquitination and biological process this GO :0032026 and response to magnesium ion and biological process this GO :0032570 and response to progesterone and biological process this GO :0032651 and regulation of interleukin-1 beta production and biological process this GO :0033198 and response to ATP and biological process this GO :0035556 and intracellular signal transduction and biological process this GO :0042346 and positive regulation of NF-kappaB import into nucleus and biological process this GO :0043005 and neuron projection and cellular component this GO :0043066 and negative regulation of apoptotic process and biological process this GO :0044267 and cellular protein metabolic process and biological process this GO :0044281 and small molecule metabolic process and biological process this GO :0045087 and innate immune response and biological process this GO :0045766 and positive regulation of angiogenesis and biological process this GO :0045931 and positive regulation of mitotic cell cycle and biological process this GO :0045987 and positive regulation of smooth muscle contraction and biological process this GO :0046521 and sphin this GO id catabolic process and biological process this GO :0046834 and lipid phosphorylation and biological process this GO :0048146 and positive regulation of fibroblast proliferation and biological process this GO :0051084 and 'de novo' posttranslational protein folding and biological process this GO :0051092 and positive regulation of NF-kappaB transcription factor activity and biological process this GO :0051721 and protein phosphatase 2A binding and molecular function this GO :0070301 and cellular response to hydrogen peroxide and biological process this GO :0070555 and response to interleukin-1 and biological process this GO :0071363 and cellular response to growth factor stimulus and biological process, this GO :0000287 : magnesium ion binding, this GO :0000287 : magnesium ion binding and also this GO :0003677 : DNA binding and also this GO :0003951 : NAD+ kinase activity and also this GO :0004143 : diacylglycerol kinase activity and also this GO :0005515 : protein binding and also this GO :0005516 : calmodulin binding and also this GO :0005524 : ATP binding and also this GO :0008481 : sphinganine kinase activity and also this GO :0017050 : D-erythro-sphin this GO sine kinase activity and also this GO :0051721 : protein phosphatase 2A binding, this GO :0003677 : DNA binding, this GO :0003951 : NAD+ kinase activity, this GO :0004143 : diacylglycerol kinase activity, this GO :0005515 : protein binding, this GO :0005516 : calmodulin binding, this GO :0005524 : ATP binding, this GO :0008481 : sphinganine kinase activity, this GO :0017050 : D-erythro-sphin this GO sine kinase activity, this GO :0051721 : protein phosphatase 2A binding, sphin this GO sine kinase 1 |
| Identity: |
11240 |
| Gene: |
SPHK1 |
More about : SPHK1 |
| Long gene name: |
sphingosine kinase 1 |
| Synonyms: |
SPHK |
| Locus: |
17q25, 2 |
| Discovery year: |
1999-02-01 |
| GenBank acession: |
BC030553 |
| Entrez gene record: |
8877 |
| Pubmed identfication: |
9726979 |
| RefSeq identity: |
NM_182965 NM_021972 |
| Havana BLAST/BLAT: |
OTTHUMG00000180177 |