| Gene info: |
Activated caspase 9 stimulates the subsequent caspase cascade that commits the cell to apoptosis, Alternative splicing results in several transcript variants encoding different isoforms, Apaf-1 is a cytoplasmic protein that initiates apoptosis, The apoptosome binds and cleaves caspase 9 preproprotein, This protein contains several copies of the WD-40 domain, Upon binding cytochrome c and dATP, a caspase recruitment domain (CARD), activated form, and an ATPase domain (NB-ARC), releasing its mature, this protein forms an oligomeric apoptosome, Apoptotic protease activating factor 1 |
| Gene names: |
APAF-1, Apoptotic protease-activating factor 1APAF-1, CED4, KIAA0413 |
| Antibodie's label: |
unlabelled serum |
| Purity: |
Antigen affinity-purified |
| Ab raised in: |
Chicken |
| Type: |
Polyclonal |
| Antigen: |
307-388 |
| Immuno application: |
Western Blot |
| Antibody advice: |
may also work for IHC and ICC, Apaf-1 antibody can be used for the detection of Apaf-1 by Western blot |
| Suspention, pH, azide: |
No preservatives added, Phosphate-Buffered Saline |
| Keep at: |
Antibodies should not be exposed to prolonged high temperatures, As with all antibodies care should be taken to avoid repeated freeze thaw cycles, Long term storage should be at -20 grades C, APAF1 antibody can be stored at 4 grades C for short term (weeks) |
| Works with: |
Mouse, Human |
| Omim nr: |
32483361 |
| Ncbi nr: |
1, NP_863658 |
| KDa: |
141, 8 kDa (calculated) |
| Vial with antibody: |
in solution |
| Quantity per ml: |
1 mg/mL |
| NCBI GENE nr: |
317 |
| GENE symbol: |
APAF1 |
| Antigene name: |
apoptotic peptidase activating factor 1 |
| Source: |
Homo sapiens |
| Protein nr: |
O14727 |
| Description: |
Freeze thaw will destroy a percentage in every cycle and should be avoided, C in a fridge short term in a concentrated dilution, This antibody needs to be stored at + 4° |
| Properties: |
C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by proscience they should be stored frozen at - 24° |
| Gene target: |
APAF1 |
| Short name: |
anti-APAF1 Antibody |
| Technique: |
antibodies against human proteins, antibodies for, antibody to, Antibody |
| Alternative name: |
antibody to-apoptotic peptidase activating factor 1 (Antibody to) |
| Alternative technique: |
antibodies |
| Alternative to gene target: |
ADP binding, APAF-1 and CED4, APAF1 and IDBG-52364 and ENSG00000120868 and 317, APAF1 and IDBG-636787 and ENSBTAG00000021661 and 537782, Apaf1 and IDBG-185032 and ENSMUSG00000019979 and 11783, nuclei, this GO :0000166 and nucleotide binding and molecular function this GO :0001843 and neural tube closure and biological process this GO :0005515 and protein binding and molecular function this GO :0005524 and ATP binding and molecular function this GO :0005622 and intracellular and cellular component this GO :0005634 and nucleus and cellular component this GO :0005829 and cytosol and cellular component this GO :0006915 and apoptotic process and biological process this GO :0006919 and activation of cysteine-type endopeptidase activity involved in apoptotic process and biological process this GO :0006952 and defense response and biological process this GO :0007275 and multicellular organismal development and biological process this GO :0007399 and nervous system development and biological process this GO :0007420 and brain development and biological process this GO :0008635 and activation of cysteine-type endopeptidase activity involved in apoptotic process by cytochrome c and biological process this GO :0008656 and cysteine-type endopeptidase activator activity involved in apoptotic process and molecular function this GO :0030900 and forebrain development and biological process this GO :0042802 and identical protein binding and molecular function this GO :0042981 and regulation of apoptotic process and biological process this GO :0043065 and positive regulation of apoptotic process and biological process this GO :0043293 and apoptosome and cellular component this GO :0043531 and ADP binding and molecular function this GO :0051402 and neuron apoptotic process and biological process this GO :0070062 and extracellular vesicular exosome and cellular component this GO :0072432 and response to G1 DNA damage checkpoint signaling and biological process this GO :0097193 and intrinsic apoptotic signaling pathway and biological process this GO :1902510 and regulation of apoptotic DNA fragmentation and biological process this GO :2001235 and positive regulation of apoptotic signaling pathway and biological process, this GO :0000166 : nucleotide binding, this GO :0000166 : nucleotide binding and also this GO :0005515 : protein binding and also this GO :0005524 : ATP binding and also this GO :0008656 : cysteine-type endopeptidase activator activity involved in apoptotic process and also this GO :0042802 : identical protein binding and also this GO :0043531 : ADP binding, this GO :0005515 : protein binding, this GO :0005524 : ATP binding, this GO :0008656 : cysteine-type endopeptidase activator activity involved in apoptotic process, this GO :0042802 : identical protein binding, this GO :0043531 : ADP binding, apoptotic peptidase activating factor 1 |
| Identity: |
576 |
| Gene: |
APAF1 |
More about : APAF1 |
| Long gene name: |
apoptotic peptidase activating factor 1 |
| Synonyms gene name: |
apoptotic protease activating factor apoptotic peptidase activating factor |
| Synonyms: |
CED4 APAF-1 |
| Locus: |
12q23, 1 |
| Discovery year: |
1998-01-20 |
| GenBank acession: |
AF013263 |
| Entrez gene record: |
317 |
| Pubmed identfication: |
9267021 10702682 |
| RefSeq identity: |
NM_181861, 1 |
| Classification: |
Caspase recruitment domain containing Apoptosome WD repeat domain containing |
| Havana BLAST/BLAT: |
OTTHUMG00000170214 |