| Gene info: |
GABAA-Rs are important therapeutic targets for a range of sedative, However, Injection in oocytes or mammalian cell lines of cRNA coding for alpha and beta subunits results in the expression of functional GABAA-Rs sensitive to GABA, Lastly, The GABAA-R is a multimeric subunit complex, The various effects of the benzodiazepines in brain may also be mediated via different alpha subunits of the receptor, To date six alphas, and hypnotic agents and are implicated in several diseases including epilepsy, and substance abuse, anxiety, anxiolytic, causing a hyperpolarization of the membrane through the opening of a Cl- channel associated with the GABAA-Receptor (GABAA-R) subtype, coexpression of a gamma subunit is required for benzodiazepine modulation, depression, four betas and four gammas, have been identified, phosphorylation of beta subunits of the receptor has been shown to modulate GABAA-R function, plus alternative splicing variants of some of these subunits, Gamma-aminobutyric acid (GABA) is the primary inhibitory neurotransmitter in the central nervous system |
| Gene names: |
Gabra-6 |
| Antibodie's label: |
unlabelled serum |
| Purity: |
Affinity Purified |
| Ab raised in: |
Rabbit |
| Type: |
Polyclonal |
| Antigen: |
GABAA Receptor a6 (CT) polyclonal antibody was made against a fusion protein with the amino acid sequence representing the cytosolic loop of rat GABAA-R a6 Subunit |
| Immuno application: |
Western Blot |
| Antibody advice: |
Antibody dilutions and tissue load should be based on tissue type and expected expression level, Applications include Dot Blots (DB) and Western Blot (WB), Initial recommended range of dilutions: 1:1000 to 1:2000, Rabbit anti-GABAA Receptor a, Recognizes bovine, Suitability for Immunohistochemistry (IHC) not yet determined, When internally tested under ideal conditions the working dilutions were 1:1000 for DB and WB, human, mouse and rat GABAA-R-a, 6 (CT) does not recognize any other GABAA-R subunits as reactivity is eliminated in a, 6 knockout brain, 6-Subunit in rat brain membrane fractions, 6-subunit, GABAA Receptor antibody is specific for the 56 to 57k GABAA-R a |
| Suspention, pH, azide: |
100 ug per mL BSA and 50% glycerol, 150 mM NaCl, 100 uL in 10 mM HEPES (pH 7, 5) |
| Keep at: |
Stable for one year, but shorter term storage at &ndash, 20 grades C is also acceptable as aliquots may be taken without freeze/thawing due to the presence of 50% glycerol, 80 grades C is recommended, For long term storage &ndash |
| Works with: |
Bovine, Rat, Human |
| Omim nr: |
232127 |
| Ncbi nr: |
P30191 |
| KDa: |
57 |
| Vial with antibody: |
in solution |
| NCBI GENE nr: |
29708 |
| GENE symbol: |
Gabra6 |
| Antigene name: |
alpha 6, gamma-aminobutyric acid (GABA) A receptor |
| Source: |
Rattus norvegicus |
| Protein nr: |
P30191 |
| Description: |
Freeze thaw will destroy a percentage in every cycle and should be avoided, C in a fridge short term in a concentrated dilution, This antibody needs to be stored at + 4° |
| Properties: |
C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by proscience they should be stored frozen at - 24° |
| Gene target: |
GABA A Receptor alpha 6 |
| Short name: |
anti-GABA A Receptor alpha 6 Antibody |
| Technique: |
antibodies against human proteins, antibodies for, antibody to, Antibody |
| Alternative name: |
antibody to-GABA A Receptor a 6 (Antibody to) |
| Alternative technique: |
antibodies |