| Gene info: |
Defects in Leptin production cause severe hereditary obesity in rodents and humans, In addition to its effects on body weight, The Leptin gene is the human homolog of the gene (ob) mutant in the mouse 'obese' phenotype, and the immune and inflammatory response, angiogenesis, including the regulation of hematopoiesis, leptin has a variety of other functions, wound healing, Leptin plays a critical role in the regulation of body weight by inhibiting food intake and stimulating energy expenditure |
| Gene names: |
DNA-directed RNA polymerase subunit H |
| Antibodie's label: |
Biotin |
| Purity: |
Anti-hLeptin specific antibody was purified by affinity chromatography and then biotinylated |
| Ab raised in: |
Rabbit |
| Type: |
Polyclonal |
| Antigen: |
98%) recombinant hLeptin (human Leptin), Produced from sera of rabbits pre-immunized with highly pure (> |
| Immuno application: |
Western Blot, ELISA |
| Antibody advice: |
Sandwich: To detect hLeptin by sandwich ELISA (using 100 &, This biotinylated polyclonal antibody, Used in conjunction with compatible secondary reagents, Used in conjunction with compatible secondary reagents the detection limit for recombinant hLeptin is 1, Western Blot: To detect hLeptin by Western Blot analysis this antibody can be used at a concentration of 0, allows the detection of at least 0, allows the detection of at least 0, in conjunction with our Polyclonal Anti-Human Leptin (XP-5212) as a capture antibody, under either reducing or non-reducing conditions, #956, #956, 0 ng/lane, 0 ug/mL of this antibody is required, 1 - 0, 15 - 0, 2 - 0, 2 ng/well of recombinant hLeptin, 2 ug/mL, 25 - 1, 30 ug/mL, 4 ng/well of recombinant hLeptin, 5 - 3, ELISA: To detect hLeptin by direct ELISA (using 100 &, L/well antibody solution) a concentration of 0, L/well antibody solution) this antibody can be used at a concentration of 0 |
| Keep at: |
Avoid repeated freeze-thaw cycles, Frozen aliquots are stable for at least 6 months when stored at -20 grades C, The reconstituted antibody is stable for at least two weeks at 2-8 grades C, Leptin antibody is stable for at least 2 years from date of receipt at -20 grades C |
| Works with: |
Human |
| Omim nr: |
1173164 |
| Ncbi nr: |
P41559 |
| Vial with antibody: |
Lyophilized |
| NCBI GENE nr: |
5325114 |
| GENE symbol: |
rpoH |
| Antigene name: |
DNA-directed RNA polymerase subunit H |
| Source: |
Methanococcus vannielii SB |
| Protein nr: |
P41559 |
| Description: |
Freeze thaw will destroy a percentage in every cycle and should be avoided, C in a fridge short term in a concentrated dilution, This antibody needs to be stored at + 4° |
| Properties: |
C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by proscience they should be stored frozen at - 24° |
| Gene: |
OB |
More about : OB |
| Gene target: |
Leptin |
| Short name: |
anti-Leptin Antibody |
| Technique: |
antibodies against human proteins, antibodies for, antibody to, Antibody |
| Alternative name: |
antibody to-Leptin (Antibody to) |
| Alternative technique: |
antibodies |
| Identity: |
8098 |
| Long gene name: |
leptin |
| Synonyms gene: |
OBS |
| Synonyms gene name: |
leptin (murine obesity homolog) leptin (obesity homolog, mouse) |
| Synonyms: |
LEP |
| Locus: |
7q32, 1 |
| Discovery year: |
1993-01-26 |
| Entrez gene record: |
3952 |
| Pubmed identfication: |
1686014 16932309 |
| Havana BLAST/BLAT: |
OTTHUMG00000157564 |