| Antigen detectable with this ELISA kit: |
WNT1 Inducible Signaling Pathway Protein 1 (WISP1) |
| Species reactivity: |
Homo sapiens (Human) |
| Range of detection: |
1000pg/mL, 125pg/mL, 250pg/mL, 31, 500pg/mL, 62, 25-2000pg/mL The standard curve concentrations used for the ELISA&rsquo, 25pg/mL, 31, 5pg/mL, s were 2000pg/mL |
| Sensitivity: |
8pg/mL, The minimum detectable dose of this kit is typically less than 11 |
| Application: |
ELISA |
| Sample types tested for this ELISA kit: |
plasma, tissue homogenates and other biological fluids, Serum |
| Time for performing t hourse test: |
4, 5 hoursours |
| Test principle: |
10nm, After TMB substrate solution is added, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated, Next, Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to WNT1 Inducible Signaling Pathway Protein 1 (WISP1), The concentration of WNT1 Inducible Signaling Pathway Protein 1 (WISP1) in the samples is then determined by comparing the O, The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm &, The microtiter plate provided in this kit has been pre-coated with an antibody specific to WNT1 Inducible Signaling Pathway Protein 1 (WISP1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color, of the samples to the standard curve, only those wells that contain WNT1 Inducible Signaling Pathway Protein 1 (WISP1), D, The test principle applied in this kit is Sandwich enzyme immunoassay, plusmn |
| Storage and handling: |
Do not freeze any of the components of the ELISA kit, store all of the kit's components regrigerated at tempretaures between 2 and 6 degrees Celcius, Upon receipt |
| Advisory: |
Prior to use, briefly centrifuge these liquid components to collect all of the amount on the bottom of the vial, Due to transportation and handling issues small amounts of the liquid components of the ELISA kit may get trapped onto the seal or walls of the tubes |
| Properties: |
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays, E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays |
| Test: |
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED |
| Description: |
By understanding cell signaling, Errors in cellular information processing are responsible for diseases such as cancer, The ability of cells to perceive and correctly respond to their microenvironment is the basis of development, and diabetes, and immunity as well as normal tissue homeostasis, artificial tissues may be created, autoimmunity, diseases may be treated effectively and, theoretically, tissue repair, Cell nucleus signaling proteins and molecules are part of a complex system of communication that governs basic cellular activities and coordinates cell actions |
| Gene target: |
WNT1 Signaling Pathway Protein 1 (WISP1) |
| Short name: |
WNT1 Signaling Pathway Protein 1 (WISP1) ELISA kit |
| Technique: |
&hellip, A sign detected by the Colorimetric detection OD optical density absorbance at 450 nm between 0 and 4 with an ELISA reader for 450 nm absortion, Also Enzyme-linked immunoassay, ELISA test kits can be sandwich ELISA, ELISA tests are enzyme linked immunoassays to detect human, ELISAs Enzyme-linked immunosorbent assay 90320007 SNOMED code are used by the medical researcher for detection of human, Genprice Inc, In sandwich ELISAS Enzyme-linked immunosorbent assay 9we use captor and detector antibodies, In single antibody ELISAs the antigen is coated and only a detector antibody is used, In this case the ratio on your graph will be inverse proportional with the antigen present in your sample, MeSH code E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay, MeSH code E05 478 566 350 170 for Enzyme-Linked Immunosorbent Assay or ELISA kits, PROMT code 3841327 Immunoabsorbent assay tests, This is the desigation of other diagnostic analyzers and tests, Traditional competition antigen ELISAs are coated with a captor antibody and a competitive antigen is labelled with the chromogen, competitive have the SNOMED name 61461007 and the PROMT code for ELISA readers is 3841330, mouse, mouse or other proteins in serum, plasma, proteins are supplied in coated 96 well plates to be stored at +4°, the recognized authority tax ID 45-4304622 D-U-N-S number - 078440800, urine or biological fluids, C, ELISA kit |
| Host: |
antigen assay ELISA kit 1 Kit (96 Wells) |
| Alternative name: |
member 1 Inducible Signaling Pathway Protein 1 (wingless-classification MMTV integration site family, member 1 inducible signaling pathway protein 1) Enzyme-linked immunosorbent assay reagent, wingless-classification MMTV integration site family |
| Alternative technique: |
elisas |
| Alternative to gene target: |
BMND16 and INT1 and OI15, BT, DNA-templated and biological process this GO :0045944 and positive regulation of transcription from RNA polymerase II promoter and biological process this GO :0048018 and receptor a this GO nist activity and molecular function this GO :0048146 and positive regulation of fibroblast proliferation and biological process this GO :0048663 and neuron fate commitment and biological process this GO :0048664 and neuron fate determination and biological process this GO :0051091 and positive regulation of sequence-specific DNA binding transcription factor activity and biological process this GO :0060061 and Spemann organizer formation and biological process this GO :0060070 and canonical Wnt signaling pathway and biological process this GO :0060348 and bone development and biological process this GO :0061184 and positive regulation of dermatome development and biological process this GO :0070365 and hepatocyte differentiation and biological process this GO :0071375 and cellular response to peptide hormone stimulus and biological process this GO :0071425 and hematopoietic stem cell proliferation and biological process this GO :0090344 and negative regulation of cell aging and biological process this GO :2000059 and negative regulation of protein ubiquitination involved in ubiquitin-dependent protein catabolic process and biological process, Extracellular, Extracellular, WISP1 and IDBG-36325 and ENSG00000104415 and 8840, WISP1 and IDBG-644060 and ENSBTAG00000000707 and 539163, WNT1 and IDBG-30719 and ENSG00000125084 and 7471, WNT1 inducible signaling pathway protein 1, Wisp1 and IDBG-146803 and ENSMUSG00000005124 and 22402, Wnt1 and IDBG-181251 and ENSMUSG00000022997 and 22408, insulin-like growth factor binding, member 1, receptor a this GO nist activity, this GO :0000578 and embryonic axis specification and biological process this GO :0001658 and branching involved in ureteric bud morphogenesis and biological process this GO :0001934 and positive regulation of protein phosphorylation and biological process this GO :0005102 and receptor binding and molecular function this GO :0005109 and frizzled binding and molecular function this GO :0005125 and cytokine activity and molecular function this GO :0005515 and protein binding and molecular function this GO :0005576 and extracellular region and cellular component this GO :0005578 and proteinaceous extracellular matrix and cellular component this GO :0005615 and extracellular space and cellular component this GO :0005737 and cytoplasm and cellular component this GO :0005788 and endoplasmic reticulum lumen and cellular component this GO :0005796 and this GO lgi lumen and cellular component this GO :0005886 and plasma membrane and cellular component this GO :0007267 and cell-cell signaling and biological process this GO :0007275 and multicellular organismal development and biological process this GO :0007520 and myoblast fusion and biological process this GO :0008284 and positive regulation of cell proliferation and biological process this GO :0009611 and response to wounding and biological process this GO :0009986 and cell surface and cellular component this GO :0010592 and positive regulation of lamellipodium assembly and biological process this GO :0010812 and negative regulation of cell-substrate adhesion and biological process this GO :0014902 and myotube differentiation and biological process this GO :0016055 and Wnt signaling pathway and biological process this GO :0019904 and protein domain specific binding and molecular function this GO :0021527 and spinal cord association neuron differentiation and biological process this GO :0021536 and diencephalon development and biological process this GO :0021551 and central nervous system morphogenesis and biological process this GO :0021588 and cerebellum formation and biological process this GO :0021797 and forebrain anterior/posterior pattern specification and biological process this GO :0022004 and midbrain-hindbrain boundary maturation during brain development and biological process this GO :0022037 and metencephalon development and biological process this GO :0022408 and negative regulation of cell-cell adhesion and biological process this GO :0030182 and neuron differentiation and biological process this GO :0030512 and negative regulation of transforming growth factor beta receptor signaling pathway and biological process this GO :0030514 and negative regulation of BMP signaling pathway and biological process this GO :0030579 and ubiquitin-dependent SMAD protein catabolic process and biological process this GO :0030901 and midbrain development and biological process this GO :0030917 and midbrain-hindbrain boundary development and biological process this GO :0031100 and organ regeneration and biological process this GO :0033077 and T cell differentiation in thymus and biological process this GO :0042472 and inner ear morphogenesis and biological process this GO :0042770 and signal transduction in response to DNA damage and biological process this GO :0043568 and positive regulation of insulin-like growth factor receptor signaling pathway and biological process this GO :0044212 and transcription regulatory region DNA binding and molecular function this GO :0044336 and canonical Wnt signaling pathway involved in negative regulation of apoptotic process and biological process this GO :0045165 and cell fate commitment and biological process this GO :0045596 and negative regulation of cell differentiation and biological process this GO :0045599 and negative regulation of fat cell differentiation and biological process this GO :0045747 and positive regulation of Notch signaling pathway and biological process this GO :0045893 and positive regulation of transcription, this GO :0001558 and regulation of cell growth and biological process this GO :0005515 and protein binding and molecular function this GO :0005520 and insulin-like growth factor binding and molecular function this GO :0005576 and extracellular region and cellular component this GO :0005615 and extracellular space and cellular component this GO :0005737 and cytoplasm and cellular component this GO :0007155 and cell adhesion and biological process this GO :0007165 and signal transduction and biological process this GO :0007267 and cell-cell signaling and biological process this GO :0016055 and Wnt signaling pathway and biological process, this GO :0005102 : receptor binding, this GO :0005102 : receptor binding and also this GO :0005109 : frizzled binding and also this GO :0005125 : cytokine activity and also this GO :0005515 : protein binding and also this GO :0019904 : protein domain specific binding and also this GO :0044212 : transcription regulatory region DNA binding and also this GO :0048018 : receptor a this GO nist activity, this GO :0005109 : frizzled binding, this GO :0005125 : cytokine activity, this GO :0005515 : protein binding, this GO :0005515 : protein binding, this GO :0005515 : protein binding and also this GO :0005520 : insulin-like growth factor binding, this GO :0005520 : insulin-like growth factor binding, this GO :0019904 : protein domain specific binding, this GO :0044212 : transcription regulatory region DNA binding, this GO :0048018 : receptor a this GO nist activity, 101628 and IDBG-632755 and ENSBTAG00000015364 and 540662, wingless-type MMTV integration site family |
| Identity: |
12769 |
| Gene: |
WISP1 |
More about : WISP1 |
| Long gene name: |
WNT1 inducible signaling pathway protein 1 |
| Synonyms: |
CCN4 |
| Locus: |
8q24, 22 |
| Discovery year: |
1999-01-22 |
| GenBank acession: |
AF100779 |
| Entrez gene record: |
8840 |
| Pubmed identfication: |
9843955 |
| RefSeq identity: |
NM_003882 |
| Classification: |
CYR61/CTGF/NOV matricellular proteins |
| Havana BLAST/BLAT: |
OTTHUMG00000164440 |