| Antigen detectable with this ELISA kit: |
Wilms Tumor Protein (WT1) |
| Species reactivity: |
Homo sapiens (Human) |
| Range of detection: |
0, 0, 0, 1, 2, 5ng/mL, 156-10ng/mL The standard curve concentrations used for the ELISA&rsquo, 156ng/mL, 25ng/mL, 313ng/mL, 5ng/mL, 625ng/mL, s were 10ng/mL |
| Sensitivity: |
053ng/mL, The minimum detectable dose of this kit is typically less than 0 |
| Application: |
ELISA |
| Sample types tested for this ELISA kit: |
cell lysates and other biological fluids, Tissue homogenates |
| Time for performing t hourse test: |
4, 5 hoursours |
| Test principle: |
10nm, After TMB substrate solution is added, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated, Next, Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Wilms Tumor Protein (WT1), The concentration of Wilms Tumor Protein (WT1) in the samples is then determined by comparing the O, The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm &, The microtiter plate provided in this kit has been pre-coated with an antibody specific to Wilms Tumor Protein (WT1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color, of the samples to the standard curve, only those wells that contain Wilms Tumor Protein (WT1), D, The test principle applied in this kit is Sandwich enzyme immunoassay, plusmn |
| Storage and handling: |
Do not freeze any of the components of the ELISA kit, store all of the kit's components regrigerated at tempretaures between 2 and 6 degrees Celcius, Upon receipt |
| Advisory: |
Prior to use, briefly centrifuge these liquid components to collect all of the amount on the bottom of the vial, Due to transportation and handling issues small amounts of the liquid components of the ELISA kit may get trapped onto the seal or walls of the tubes |
| Properties: |
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays, E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays |
| Test: |
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED |
| Gene target: |
Wilms Tumor Protein (WT1) |
| Short name: |
Wilms Tumor Protein (WT1) ELISA kit |
| Technique: |
&hellip, A sign detected by the Colorimetric detection OD optical density absorbance at 450 nm between 0 and 4 with an ELISA reader for 450 nm absortion, Also Enzyme-linked immunoassay, ELISA test kits can be sandwich ELISA, ELISA tests are enzyme linked immunoassays to detect human, ELISAs Enzyme-linked immunosorbent assay 90320007 SNOMED code are used by the medical researcher for detection of human, Genprice Inc, In sandwich ELISAS Enzyme-linked immunosorbent assay 9we use captor and detector antibodies, In single antibody ELISAs the antigen is coated and only a detector antibody is used, In this case the ratio on your graph will be inverse proportional with the antigen present in your sample, MeSH code E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay, MeSH code E05 478 566 350 170 for Enzyme-Linked Immunosorbent Assay or ELISA kits, PROMT code 3841327 Immunoabsorbent assay tests, This is the desigation of other diagnostic analyzers and tests, Traditional competition antigen ELISAs are coated with a captor antibody and a competitive antigen is labelled with the chromogen, competitive have the SNOMED name 61461007 and the PROMT code for ELISA readers is 3841330, mouse, mouse or other proteins in serum, plasma, proteins are supplied in coated 96 well plates to be stored at +4°, the recognized authority tax ID 45-4304622 D-U-N-S number - 078440800, urine or biological fluids, C, ELISA kit |
| Host: |
antigen assay ELISA kit 1 Kit (96 Wells) |
| Alternative name: |
Wilms Tumor Protein (Wilms tumor 1) Enzyme-linked immunosorbent assay reagent |
| Alternative technique: |
elisas |
| Alternative to gene target: |
AWT1 and EWS-WT1 and GUD and NPHS4 and WAGR and WIT-2 and WT33, C2H2 zinc finger domain binding, DNA-templated and biological process this GO :0006355 and regulation of transcription, DNA-templated and biological process this GO :0006357 and regulation of transcription from RNA polymerase II promoter and biological process this GO :0007281 and germ cell development and biological process this GO :0007356 and thorax and anterior abdomen determination and biological process this GO :0007507 and heart development and biological process this GO :0007530 and sex determination and biological process this GO :0008270 and zinc ion binding and molecular function this GO :0008285 and negative regulation of cell proliferation and biological process this GO :0008380 and RNA splicing and biological process this GO :0008406 and this GO nad development and biological process this GO :0008584 and male this GO nad development and biological process this GO :0009888 and tissue development and biological process this GO :0016607 and nuclear speck and cellular component this GO :0017148 and negative regulation of translation and biological process this GO :0030308 and negative regulation of cell growth and biological process this GO :0030325 and adrenal gland development and biological process this GO :0030539 and male genitalia development and biological process this GO :0030855 and epithelial cell differentiation and biological process this GO :0032835 and glomerulus development and biological process this GO :0032836 and glomerular basement membrane development and biological process this GO :0035802 and adrenal cortex formation and biological process this GO :0043010 and camera-type eye development and biological process this GO :0043066 and negative regulation of apoptotic process and biological process this GO :0043565 and sequence-specific DNA binding and molecular function this GO :0044212 and transcription regulatory region DNA binding and molecular function this GO :0045892 and negative regulation of transcription, DNA-templated and biological process this GO :0045893 and positive regulation of transcription, DNA-templated and biological process this GO :0045944 and positive regulation of transcription from RNA polymerase II promoter and biological process this GO :0046872 and metal ion binding and molecular function this GO :0060231 and mesenchymal to epithelial transition and biological process this GO :0060421 and positive regulation of heart growth and biological process this GO :0060539 and diaphragm development and biological process this GO :0060923 and cardiac muscle cell fate commitment and biological process this GO :0061032 and visceral serous pericardium development and biological process this GO :0070742 and C2H2 zinc finger domain binding and molecular function this GO :0071320 and cellular response to cAMP and biological process this GO :0071371 and cellular response to this GO nadotropin stimulus and biological process this GO :0072075 and metanephric mesenchyme development and biological process this GO :0072112 and glomerular visceral epithelial cell differentiation and biological process this GO :0072166 and posterior mesonephric tubule development and biological process this GO :0072207 and metanephric epithelium development and biological process this GO :0072284 and metanephric S-shaped body morphogenesis and biological process this GO :0072302 and negative regulation of metanephric glomerular mesangial cell proliferation and biological process this GO :1900212 and negative regulation of mesenchymal cell apoptotic process involved in metanephros development and biological process this GO :2000020 and positive regulation of male this GO nad development and biological process this GO :2000195 and negative regulation of female this GO nad development and biological process this GO :2001076 and positive regulation of metanephric ureteric bud development and biological process, WT1 and IDBG-37982 and ENSG00000184937 and 7490, WT1 and IDBG-636230 and ENSBTAG00000047268 and 782183, Wt1 and IDBG-194872 and ENSMUSG00000016458 and 22431, nuclei, this GO :0000122 and negative regulation of transcription from RNA polymerase II promoter and biological process this GO :0001077 and RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription and molecular function this GO :0001570 and vasculogenesis and biological process this GO :0001656 and metanephros development and biological process this GO :0001657 and ureteric bud development and biological process this GO :0001658 and branching involved in ureteric bud morphogenesis and biological process this GO :0001822 and kidney development and biological process this GO :0001823 and mesonephros development and biological process this GO :0003156 and regulation of organ formation and biological process this GO :0003676 and nucleic acid binding and molecular function this GO :0003690 and double-stranded DNA binding and molecular function this GO :0003700 and sequence-specific DNA binding transcription factor activity and molecular function this GO :0003723 and RNA binding and molecular function this GO :0005515 and protein binding and molecular function this GO :0005634 and nucleus and cellular component this GO :0005654 and nucleoplasm and cellular component this GO :0005737 and cytoplasm and cellular component this GO :0006309 and apoptotic DNA fragmentation and biological process this GO :0006351 and transcription, this GO :0001077 : RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription, this GO :0001077 : RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription and also this GO :0003676 : nucleic acid binding and also this GO :0003690 : double-stranded DNA binding and also this GO :0003700 : sequence-specific DNA binding transcription factor activity and also this GO :0003723 : RNA binding and also this GO :0005515 : protein binding and also this GO :0008270 : zinc ion binding and also this GO :0043565 : sequence-specific DNA binding and also this GO :0044212 : transcription regulatory region DNA binding and also this GO :0046872 : metal ion binding and also this GO :0070742 : C2H2 zinc finger domain binding, this GO :0003676 : nucleic acid binding, this GO :0003690 : double-stranded DNA binding, this GO :0003700 : sequence-specific DNA binding transcription factor activity, this GO :0003723 : RNA binding, this GO :0005515 : protein binding, this GO :0008270 : zinc ion binding, this GO :0043565 : sequence-specific DNA binding, this GO :0044212 : transcription regulatory region DNA binding, this GO :0046872 : metal ion binding, this GO :0070742 : C2H2 zinc finger domain binding, Wilms tumor 1 |
| Identity: |
12796 |
| Gene: |
WT1 |
More about : WT1 |
| Long gene name: |
Wilms tumor 1 |
| Synonyms gene: |
GUD |
| Synonyms: |
WAGR WIT-2 AWT1 NPHS4 |
| Locus: |
11p13 |
| Discovery year: |
1989-04-13 |
| Entrez gene record: |
7490 |
| Pubmed identfication: |
14681303 |
| RefSeq identity: |
NM_000378 |
| Classification: |
Zinc fingers C2H2-type |
| Havana BLAST/BLAT: |
OTTHUMG00000039556 |
| Locus Specific Databases: |
LRG_525 |