| Antigen detectable with this ELISA kit: |
Vascular Cell Adhesion Molecule 1 (VCAM1) |
| Species reactivity: |
Rhesus monkey (Simian) |
| Range of detection: |
0, 0, 1, 10ng/mL, 2, 5ng/mL, 25ng/mL, 313-20ng/mL The standard curve concentrations used for the ELISA&rsquo, 313ng/mL, 5ng/mL, 625ng/mL, s were 20ng/mL |
| Sensitivity: |
133ng/mL, The minimum detectable dose of this kit is typically less than 0 |
| Application: |
ELISA |
| Sample types tested for this ELISA kit: |
cell culture supernates and other biological fluids, cell lysates, plasma, tissue homogenates, Serum |
| Time for performing t hourse test: |
4, 5 hoursours |
| Test principle: |
10nm, After TMB substrate solution is added, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated, Next, Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Vascular Cell Adhesion Molecule 1 (VCAM1), The concentration of Vascular Cell Adhesion Molecule 1 (VCAM1) in the samples is then determined by comparing the O, The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm &, The microtiter plate provided in this kit has been pre-coated with an antibody specific to Vascular Cell Adhesion Molecule 1 (VCAM1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color, of the samples to the standard curve, only those wells that contain Vascular Cell Adhesion Molecule 1 (VCAM1), D, The test principle applied in this kit is Sandwich enzyme immunoassay, plusmn |
| Storage and handling: |
Do not freeze any of the components of the ELISA kit, store all of the kit's components regrigerated at tempretaures between 2 and 6 degrees Celcius, Upon receipt |
| Advisory: |
Prior to use, briefly centrifuge these liquid components to collect all of the amount on the bottom of the vial, Due to transportation and handling issues small amounts of the liquid components of the ELISA kit may get trapped onto the seal or walls of the tubes |
| Properties: |
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays, E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays |
| Test: |
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED |
| Description: |
present in lysates used as reference for ELISA quantification of these molecules and their subunits, Whole adhesion and interacting molecules are |
| Gene target: |
Vascular Adhesion Molecule 1 (VCAM1) |
| Short name: |
Vascular Cell Adhesion Molecule 1 (VCAM1) ELISA kit |
| Technique: |
&hellip, A sign detected by the Colorimetric detection OD optical density absorbance at 450 nm between 0 and 4 with an ELISA reader for 450 nm absortion, Also Enzyme-linked immunoassay, ELISA test kits can be sandwich ELISA, ELISA tests are enzyme linked immunoassays to detect human, ELISAs Enzyme-linked immunosorbent assay 90320007 SNOMED code are used by the medical researcher for detection of human, Genprice Inc, In sandwich ELISAS Enzyme-linked immunosorbent assay 9we use captor and detector antibodies, In single antibody ELISAs the antigen is coated and only a detector antibody is used, In this case the ratio on your graph will be inverse proportional with the antigen present in your sample, MeSH code E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay, MeSH code E05 478 566 350 170 for Enzyme-Linked Immunosorbent Assay or ELISA kits, PROMT code 3841327 Immunoabsorbent assay tests, This is the desigation of other diagnostic analyzers and tests, Traditional competition antigen ELISAs are coated with a captor antibody and a competitive antigen is labelled with the chromogen, competitive have the SNOMED name 61461007 and the PROMT code for ELISA readers is 3841330, mouse, mouse or other proteins in serum, plasma, proteins are supplied in coated 96 well plates to be stored at +4°, the recognized authority tax ID 45-4304622 D-U-N-S number - 078440800, urine or biological fluids, C, ELISA kit |
| Host: |
antigen assay ELISA kit 1 Kit (96 Wells) |
| Alternative name: |
Vascular cellular Adhesion Molecule 1 (vascular cellular adhesion molecule 1) Enzyme-linked immunosorbent assay reagent |
| Alternative technique: |
elisas |
| Alternative to gene target: |
CD106 and INCAM-100, Extracellular, VCAM1 and IDBG-100525 and ENSG00000162692 and 7412, Vcam1 and IDBG-189855 and ENSMUSG00000027962 and 22329, cell adhesion molecule binding, this GO :0001666 and response to hypoxia and biological process this GO :0002102 and podosome and cellular component this GO :0002526 and acute inflammatory response and biological process this GO :0002544 and chronic inflammatory response and biological process this GO :0005178 and integrin binding and molecular function this GO :0005515 and protein binding and molecular function this GO :0005615 and extracellular space and cellular component this GO :0005769 and early endosome and cellular component this GO :0005783 and endoplasmic reticulum and cellular component this GO :0005794 and this GO lgi apparatus and cellular component this GO :0005886 and plasma membrane and cellular component this GO :0005902 and microvillus and cellular component this GO :0007155 and cell adhesion and biological process this GO :0007157 and heterophilic cell-cell adhesion and biological process this GO :0007159 and leukocyte cell-cell adhesion and biological process this GO :0007507 and heart development and biological process this GO :0007568 and aging and biological process this GO :0007584 and response to nutrient and biological process this GO :0008131 and primary amine oxidase activity and molecular function this GO :0009308 and amine metabolic process and biological process this GO :0009897 and external side of plasma membrane and cellular component this GO :0009986 and cell surface and cellular component this GO :0010043 and response to zinc ion and biological process this GO :0010212 and response to ionizing radiation and biological process this GO :0016021 and integral component of membrane and cellular component this GO :0016032 and viral process and biological process this GO :0016337 and single organismal cell-cell adhesion and biological process this GO :0019221 and cytokine-mediated signaling pathway and biological process this GO :0022614 and membrane to membrane docking and biological process this GO :0030175 and filopodium and cellular component this GO :0030183 and B cell differentiation and biological process this GO :0030198 and extracellular matrix organization and biological process this GO :0032496 and response to lipopolysaccharide and biological process this GO :0035094 and response to nicotine and biological process this GO :0035924 and cellular response to vascular endothelial growth factor stimulus and biological process this GO :0042102 and positive regulation of T cell proliferation and biological process this GO :0042383 and sarcolemma and cellular component this GO :0045177 and apical part of cell and cellular component this GO :0045471 and response to ethanol and biological process this GO :0050776 and regulation of immune response and biological process this GO :0050839 and cell adhesion molecule binding and molecular function this GO :0050901 and leukocyte tethering or rolling and biological process this GO :0055114 and oxidation-reduction process and biological process this GO :0060326 and cell chemotaxis and biological process this GO :0060333 and interferon-gamma-mediated signaling pathway and biological process this GO :0060669 and embryonic placenta morphogenesis and biological process this GO :0060710 and chorio-allantoic fusion and biological process this GO :0070062 and extracellular vesicular exosome and cellular component this GO :0071065 and alpha9-beta1 integrin-vascular cell adhesion molecule-1 complex and cellular component this GO :0071333 and cellular response to glucose stimulus and biological process this GO :0071356 and cellular response to tumor necrosis factor and biological process this GO :0071944 and cell periphery and cellular component, this GO :0005178 : integrin binding, this GO :0005178 : integrin binding and also this GO :0005515 : protein binding and also this GO :0008131 : primary amine oxidase activity and also this GO :0050839 : cell adhesion molecule binding, this GO :0005515 : protein binding, this GO :0008131 : primary amine oxidase activity, this GO :0050839 : cell adhesion molecule binding, vascular cell adhesion molecule 1 |
| Identity: |
12663 |
| Gene: |
VCAM1 |
More about : VCAM1 |
| Long gene name: |
vascular cell adhesion molecule 1 |
| Synonyms: |
CD106 |
| Locus: |
1p21, 2 |
| Discovery year: |
1991-07-10 |
| GenBank acession: |
M60335 |
| Entrez gene record: |
7412 |
| RefSeq identity: |
NM_001078 |
| Classification: |
Endogenous ligands C2-set domain containing I-set domain containing CD molecules Immunoglobulin like domain containing |
| Havana BLAST/BLAT: |
OTTHUMG00000010982 |