| Antigen detectable with this ELISA kit: |
Topoisomerase I (TOP1) |
| Species reactivity: |
Mus musculus (Mouse) |
| Range of detection: |
0, 0, 0, 1, 2, 5ng/mL, 156-10ng/mL The standard curve concentrations used for the ELISA&rsquo, 156ng/mL, 25ng/mL, 313ng/mL, 5ng/mL, 625ng/mL, s were 10ng/mL |
| Sensitivity: |
055ng/mL, The minimum detectable dose of this kit is typically less than 0 |
| Application: |
ELISA |
| Sample types tested for this ELISA kit: |
cell lysates and other biological fluids, Tissue homogenates |
| Time for performing t hourse test: |
4, 5 hoursours |
| Test principle: |
10nm, After TMB substrate solution is added, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated, Next, Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Topoisomerase I (TOP1), The concentration of Topoisomerase I (TOP1) in the samples is then determined by comparing the O, The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm &, The microtiter plate provided in this kit has been pre-coated with an antibody specific to Topoisomerase I (TOP1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color, of the samples to the standard curve, only those wells that contain Topoisomerase I (TOP1), D, The test principle applied in this kit is Sandwich enzyme immunoassay, plusmn |
| Storage and handling: |
Do not freeze any of the components of the ELISA kit, store all of the kit's components regrigerated at tempretaures between 2 and 6 degrees Celcius, Upon receipt |
| Advisory: |
Prior to use, briefly centrifuge these liquid components to collect all of the amount on the bottom of the vial, Due to transportation and handling issues small amounts of the liquid components of the ELISA kit may get trapped onto the seal or walls of the tubes |
| Properties: |
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays, E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays |
| Test: |
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED |
| Gene target: |
Topoisomerase I (TOP1) |
| Short name: |
Topoisomerase I (TOP1) ELISA kit |
| Technique: |
&hellip, A sign detected by the Colorimetric detection OD optical density absorbance at 450 nm between 0 and 4 with an ELISA reader for 450 nm absortion, Also Enzyme-linked immunoassay, ELISA test kits can be sandwich ELISA, ELISA tests are enzyme linked immunoassays to detect human, ELISAs Enzyme-linked immunosorbent assay 90320007 SNOMED code are used by the medical researcher for detection of human, Genprice Inc, In sandwich ELISAS Enzyme-linked immunosorbent assay 9we use captor and detector antibodies, In single antibody ELISAs the antigen is coated and only a detector antibody is used, In this case the ratio on your graph will be inverse proportional with the antigen present in your sample, MeSH code E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay, MeSH code E05 478 566 350 170 for Enzyme-Linked Immunosorbent Assay or ELISA kits, PROMT code 3841327 Immunoabsorbent assay tests, This is the desigation of other diagnostic analyzers and tests, Traditional competition antigen ELISAs are coated with a captor antibody and a competitive antigen is labelled with the chromogen, competitive have the SNOMED name 61461007 and the PROMT code for ELISA readers is 3841330, mouse, mouse or other proteins in serum, plasma, proteins are supplied in coated 96 well plates to be stored at +4°, the recognized authority tax ID 45-4304622 D-U-N-S number - 078440800, urine or biological fluids, C, ELISA kit |
| Host: |
antigen assay ELISA kit 1 Kit (96 Wells) |
| Alternative name: |
Topoisomerase I (topoisomerase (Desoxyribonucleic acid) I) Enzyme-linked immunosorbent assay reagent |
| Alternative technique: |
elisas |
| Alternative to gene target: |
BT, TOP1 and IDBG-75139 and ENSG00000198900 and 7150, TOPI, Top1 and IDBG-211615 and ENSMUSG00000070544 and 21969, nuclei, poly(A) RNA binding, this GO :0000228 and nuclear chromosome and cellular component this GO :0000932 and cytoplasmic mRNA processing body and cellular component this GO :0003677 and DNA binding and molecular function this GO :0003682 and chromatin binding and molecular function this GO :0003917 and DNA topoisomerase type I activity and molecular function this GO :0003918 and DNA topoisomerase (ATP-hydrolyzing) activity and molecular function this GO :0005515 and protein binding and molecular function this GO :0005634 and nucleus and cellular component this GO :0005654 and nucleoplasm and cellular component this GO :0005694 and chromosome and cellular component this GO :0005730 and nucleolus and cellular component this GO :0005737 and cytoplasm and cellular component this GO :0006200 and ATP catabolic process and biological process this GO :0006260 and DNA replication and biological process this GO :0006265 and DNA topological change and biological process this GO :0006338 and chromatin remodeling and biological process this GO :0007059 and chromosome segregation and biological process this GO :0012501 and programmed cell death and biological process this GO :0016032 and viral process and biological process this GO :0016310 and phosphorylation and biological process this GO :0031298 and replication fork protection complex and cellular component this GO :0040016 and embryonic cleavage and biological process this GO :0042493 and response to drug and biological process this GO :0043204 and perikaryon and cellular component this GO :0044822 and poly(A) RNA binding and molecular function, this GO :0003677 : DNA binding, this GO :0003677 : DNA binding and also this GO :0003682 : chromatin binding and also this GO :0003917 : DNA topoisomerase type I activity and also this GO :0003918 : DNA topoisomerase (ATP-hydrolyzing) activity and also this GO :0005515 : protein binding and also this GO :0044822 : poly(A) RNA binding, this GO :0003682 : chromatin binding, this GO :0003917 : DNA topoisomerase type I activity, this GO :0003918 : DNA topoisomerase (ATP-hydrolyzing) activity, this GO :0005515 : protein binding, this GO :0044822 : poly(A) RNA binding, 39255 and IDBG-642660 and ENSBTAG00000007960 and 534799, topoisomerase (DNA) I |
| Identity: |
11986 |
| Gene: |
TOP1 |
More about : TOP1 |
| Long gene name: |
topoisomerase (DNA) I |
| Locus: |
20q12 |
| Discovery year: |
1989-02-23 |
| Entrez gene record: |
7150 |
| Classification: |
Topoisomerases |
| Havana BLAST/BLAT: |
OTTHUMG00000033057 |