| Antigen detectable with this ELISA kit: |
Tissue Inhibitors Of Metalloproteinase 4 (TIMP4) |
| Species reactivity: |
Homo sapiens (Human) |
| Range of detection: |
0, 0, 0, 1, 2, 5ng/mL, 156-10ng/mL The standard curve concentrations used for the ELISA&rsquo, 156ng/mL, 25ng/mL, 313ng/mL, 5ng/mL, 625ng/mL, s were 10ng/mL |
| Sensitivity: |
058ng/mL, The minimum detectable dose of this kit is typically less than 0 |
| Application: |
ELISA |
| Sample types tested for this ELISA kit: |
cell lysates and other biological fluids, plasma, tissue homogenates, Serum |
| Time for performing t hourse test: |
4, 5 hoursours |
| Test principle: |
10nm, After TMB substrate solution is added, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated, Next, Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Tissue Inhibitors Of Metalloproteinase 4 (TIMP4), The concentration of Tissue Inhibitors Of Metalloproteinase 4 (TIMP4) in the samples is then determined by comparing the O, The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm &, The microtiter plate provided in this kit has been pre-coated with an antibody specific to Tissue Inhibitors Of Metalloproteinase 4 (TIMP4), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color, of the samples to the standard curve, only those wells that contain Tissue Inhibitors Of Metalloproteinase 4 (TIMP4), D, The test principle applied in this kit is Sandwich enzyme immunoassay, plusmn |
| Storage and handling: |
Do not freeze any of the components of the ELISA kit, store all of the kit's components regrigerated at tempretaures between 2 and 6 degrees Celcius, Upon receipt |
| Advisory: |
Prior to use, briefly centrifuge these liquid components to collect all of the amount on the bottom of the vial, Due to transportation and handling issues small amounts of the liquid components of the ELISA kit may get trapped onto the seal or walls of the tubes |
| Properties: |
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays, E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays |
| Test: |
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED |
| Gene target: |
Tissue Inhibitors Of Metalloproteinase 4 (TIMP4) |
| Short name: |
Tissue Inhibitors Of Metalloproteinase 4 (TIMP4) ELISA kit |
| Technique: |
&hellip, A sign detected by the Colorimetric detection OD optical density absorbance at 450 nm between 0 and 4 with an ELISA reader for 450 nm absortion, Also Enzyme-linked immunoassay, ELISA test kits can be sandwich ELISA, ELISA tests are enzyme linked immunoassays to detect human, ELISAs Enzyme-linked immunosorbent assay 90320007 SNOMED code are used by the medical researcher for detection of human, Genprice Inc, In sandwich ELISAS Enzyme-linked immunosorbent assay 9we use captor and detector antibodies, In single antibody ELISAs the antigen is coated and only a detector antibody is used, In this case the ratio on your graph will be inverse proportional with the antigen present in your sample, MeSH code E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay, MeSH code E05 478 566 350 170 for Enzyme-Linked Immunosorbent Assay or ELISA kits, PROMT code 3841327 Immunoabsorbent assay tests, This is the desigation of other diagnostic analyzers and tests, Traditional competition antigen ELISAs are coated with a captor antibody and a competitive antigen is labelled with the chromogen, competitive have the SNOMED name 61461007 and the PROMT code for ELISA readers is 3841330, mouse, mouse or other proteins in serum, plasma, proteins are supplied in coated 96 well plates to be stored at +4°, the recognized authority tax ID 45-4304622 D-U-N-S number - 078440800, urine or biological fluids, C, ELISA kit |
| Host: |
antigen assay ELISA kit 1 Kit (96 Wells) |
| Alternative name: |
Tissue Inhibitors on Metalloproteinase 4 (TIMP metallopeptidase inhibitor 4) Enzyme-linked immunosorbent assay reagent |
| Alternative technique: |
elisas |
| Alternative to gene target: |
Extracellular, TIMP4 and IDBG-18927 and ENSG00000157150 and 7079, TIMP4 and IDBG-646400 and ENSBTAG00000020263 and 317694, Timp4 and IDBG-179215 and ENSMUSG00000030317 and 110595, metal ion binding, this GO :0005515 and protein binding and molecular function this GO :0005615 and extracellular space and cellular component this GO :0007417 and central nervous system development and biological process this GO :0008150 and biological process and biological process this GO :0008191 and metalloendopeptidase inhibitor activity and molecular function this GO :0009725 and response to hormone and biological process this GO :0010951 and negative regulation of endopeptidase activity and biological process this GO :0030017 and sarcomere and cellular component this GO :0032496 and response to lipopolysaccharide and biological process this GO :0034097 and response to cytokine and biological process this GO :0042493 and response to drug and biological process this GO :0042698 and ovulation cycle and biological process this GO :0043434 and response to peptide hormone and biological process this GO :0046872 and metal ion binding and molecular function, this GO :0005515 : protein binding, this GO :0005515 : protein binding and also this GO :0008191 : metalloendopeptidase inhibitor activity and also this GO :0046872 : metal ion binding, this GO :0008191 : metalloendopeptidase inhibitor activity, this GO :0046872 : metal ion binding, TIMP metallopeptidase inhibitor 4 |
| Identity: |
11823 |
| Gene: |
TIMP4 |
More about : TIMP4 |
| Long gene name: |
TIMP metallopeptidase inhibitor 4 |
| Synonyms gene name: |
tissue inhibitor of metalloproteinase 4 |
| Locus: |
3p25, 2 |
| Discovery year: |
1997-07-07 |
| GenBank acession: |
U76456 |
| Entrez gene record: |
7079 |
| Pubmed identfication: |
8939999 9693046 |
| RefSeq identity: |
NM_003256 |
| Classification: |
Tissue inhibitor of metallopeptidases |
| Havana BLAST/BLAT: |
OTTHUMG00000129763 |