| Antigen detectable with this ELISA kit: |
Sphingosine-1-Phosphate Phosphatase 1 (SGPP1) |
| Species reactivity: |
Rattus norvegicus (Rat) |
| Range of detection: |
0, 0, 0, 1, 2, 5ng/mL, 156-10ng/mL The standard curve concentrations used for the ELISA&rsquo, 156ng/mL, 25ng/mL, 313ng/mL, 5ng/mL, 625ng/mL, s were 10ng/mL |
| Sensitivity: |
057ng/mL, The minimum detectable dose of this kit is typically less than 0 |
| Application: |
ELISA |
| Sample types tested for this ELISA kit: |
Tissue homogenates and other biological fluids |
| Time for performing t hourse test: |
4, 5 hoursours |
| Test principle: |
10nm, After TMB substrate solution is added, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated, Next, Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Sphingosine-1-Phosphate Phosphatase 1 (SGPP1), The concentration of Sphingosine-1-Phosphate Phosphatase 1 (SGPP1) in the samples is then determined by comparing the O, The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm &, The microtiter plate provided in this kit has been pre-coated with an antibody specific to Sphingosine-1-Phosphate Phosphatase 1 (SGPP1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color, of the samples to the standard curve, only those wells that contain Sphingosine-1-Phosphate Phosphatase 1 (SGPP1), D, The test principle applied in this kit is Sandwich enzyme immunoassay, plusmn |
| Storage and handling: |
Do not freeze any of the components of the ELISA kit, store all of the kit's components regrigerated at tempretaures between 2 and 6 degrees Celcius, Upon receipt |
| Advisory: |
Prior to use, briefly centrifuge these liquid components to collect all of the amount on the bottom of the vial, Due to transportation and handling issues small amounts of the liquid components of the ELISA kit may get trapped onto the seal or walls of the tubes |
| Properties: |
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays, E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays |
| Test: |
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED |
| Gene target: |
Sphingosine-1-Phosphate Phosphatase 1 (SGPP1) |
| Short name: |
Sphingosine-1-Phosphate Phosphatase 1 (SGPP1) ELISA kit |
| Technique: |
&hellip, A sign detected by the Colorimetric detection OD optical density absorbance at 450 nm between 0 and 4 with an ELISA reader for 450 nm absortion, Also Enzyme-linked immunoassay, ELISA test kits can be sandwich ELISA, ELISA tests are enzyme linked immunoassays to detect human, ELISAs Enzyme-linked immunosorbent assay 90320007 SNOMED code are used by the medical researcher for detection of human, Genprice Inc, In sandwich ELISAS Enzyme-linked immunosorbent assay 9we use captor and detector antibodies, In single antibody ELISAs the antigen is coated and only a detector antibody is used, In this case the ratio on your graph will be inverse proportional with the antigen present in your sample, MeSH code E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay, MeSH code E05 478 566 350 170 for Enzyme-Linked Immunosorbent Assay or ELISA kits, PROMT code 3841327 Immunoabsorbent assay tests, This is the desigation of other diagnostic analyzers and tests, Traditional competition antigen ELISAs are coated with a captor antibody and a competitive antigen is labelled with the chromogen, competitive have the SNOMED name 61461007 and the PROMT code for ELISA readers is 3841330, mouse, mouse or other proteins in serum, plasma, proteins are supplied in coated 96 well plates to be stored at +4°, the recognized authority tax ID 45-4304622 D-U-N-S number - 078440800, urine or biological fluids, C, ELISA kit |
| Host: |
antigen assay ELISA kit 1 Kit (96 Wells) |
| Alternative name: |
Sphingosine-1-Phosphate Phosphatase 1 (sphin this GO sine-1-phosphate phosphatase 1) Enzyme-linked immunosorbent assay reagent |
| Alternative technique: |
elisas |
| Alternative to gene target: |
Plasma membranes, SGPP1 and IDBG-646765 and ENSBTAG00000004430 and 516881, SGPP1 and IDBG-8767 and ENSG00000126821 and 81537, Sgpp1 and IDBG-149469 and ENSMUSG00000021054 and 81535, sphin this GO sine-1-phosphate phosphatase activity, this GO :0003824 and catalytic activity and molecular function this GO :0005783 and endoplasmic reticulum and cellular component this GO :0005789 and endoplasmic reticulum membrane and cellular component this GO :0006665 and sphin this GO lipid metabolic process and biological process this GO :0006668 and sphinganine-1-phosphate metabolic process and biological process this GO :0006670 and sphin this GO sine metabolic process and biological process this GO :0016020 and membrane and cellular component this GO :0016021 and integral component of membrane and cellular component this GO :0016311 and dephosphorylation and biological process this GO :0030148 and sphin this GO lipid biosynthetic process and biological process this GO :0042392 and sphin this GO sine-1-phosphate phosphatase activity and molecular function this GO :0044281 and small molecule metabolic process and biological process this GO :0097191 and extrinsic apoptotic signaling pathway and biological process this GO :0097193 and intrinsic apoptotic signaling pathway and biological process, this GO :0003824 : catalytic activity, this GO :0003824 : catalytic activity and also this GO :0042392 : sphin this GO sine-1-phosphate phosphatase activity, this GO :0042392 : sphin this GO sine-1-phosphate phosphatase activity, sphin this GO sine-1-phosphate phosphatase 1 |
| Identity: |
17720 |
| Gene: |
SGPP1 |
More about : SGPP1 |
| Long gene name: |
sphingosine-1-phosphate phosphatase 1 |
| Locus: |
14q23, 2 |
| Discovery year: |
2003-01-24 |
| GenBank acession: |
AJ293294 |
| Entrez gene record: |
81537 |
| Pubmed identfication: |
10859351 |
| RefSeq identity: |
NM_030791 |
| Classification: |
Sphingosine-1-phosphate phosphatases |
| Havana BLAST/BLAT: |
OTTHUMG00000029080 |