| Antigen detectable with this ELISA kit: |
Sphingosine 1 Phosphate Lyase 1 (SGPL1) |
| Species reactivity: |
Mus musculus (Mouse) |
| Range of detection: |
0, 0, 0, 1, 2, 5ng/mL, 156-10ng/mL The standard curve concentrations used for the ELISA&rsquo, 156ng/mL, 25ng/mL, 313ng/mL, 5ng/mL, 625ng/mL, s were 10ng/mL |
| Sensitivity: |
055ng/mL, The minimum detectable dose of this kit is typically less than 0 |
| Application: |
ELISA |
| Sample types tested for this ELISA kit: |
Tissue homogenates and other biological fluids |
| Time for performing t hourse test: |
4, 5 hoursours |
| Test principle: |
10nm, After TMB substrate solution is added, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated, Next, Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Sphingosine 1 Phosphate Lyase 1 (SGPL1), The concentration of Sphingosine 1 Phosphate Lyase 1 (SGPL1) in the samples is then determined by comparing the O, The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm &, The microtiter plate provided in this kit has been pre-coated with an antibody specific to Sphingosine 1 Phosphate Lyase 1 (SGPL1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color, of the samples to the standard curve, only those wells that contain Sphingosine 1 Phosphate Lyase 1 (SGPL1), D, The test principle applied in this kit is Sandwich enzyme immunoassay, plusmn |
| Storage and handling: |
Do not freeze any of the components of the ELISA kit, store all of the kit's components regrigerated at tempretaures between 2 and 6 degrees Celcius, Upon receipt |
| Advisory: |
Prior to use, briefly centrifuge these liquid components to collect all of the amount on the bottom of the vial, Due to transportation and handling issues small amounts of the liquid components of the ELISA kit may get trapped onto the seal or walls of the tubes |
| Properties: |
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays, E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays |
| Test: |
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED |
| Gene target: |
Sphingosine 1 Phosphate Lyase 1 (SGPL1) |
| Short name: |
Sphingosine 1 Phosphate Lyase 1 (SGPL1) ELISA kit |
| Technique: |
&hellip, A sign detected by the Colorimetric detection OD optical density absorbance at 450 nm between 0 and 4 with an ELISA reader for 450 nm absortion, Also Enzyme-linked immunoassay, ELISA test kits can be sandwich ELISA, ELISA tests are enzyme linked immunoassays to detect human, ELISAs Enzyme-linked immunosorbent assay 90320007 SNOMED code are used by the medical researcher for detection of human, Genprice Inc, In sandwich ELISAS Enzyme-linked immunosorbent assay 9we use captor and detector antibodies, In single antibody ELISAs the antigen is coated and only a detector antibody is used, In this case the ratio on your graph will be inverse proportional with the antigen present in your sample, MeSH code E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay, MeSH code E05 478 566 350 170 for Enzyme-Linked Immunosorbent Assay or ELISA kits, PROMT code 3841327 Immunoabsorbent assay tests, This is the desigation of other diagnostic analyzers and tests, Traditional competition antigen ELISAs are coated with a captor antibody and a competitive antigen is labelled with the chromogen, competitive have the SNOMED name 61461007 and the PROMT code for ELISA readers is 3841330, mouse, mouse or other proteins in serum, plasma, proteins are supplied in coated 96 well plates to be stored at +4°, the recognized authority tax ID 45-4304622 D-U-N-S number - 078440800, urine or biological fluids, C, ELISA kit |
| Host: |
antigen assay ELISA kit 1 Kit (96 Wells) |
| Alternative name: |
Sphingosine 1 Phosphate Lyase 1 (sphin this GO sine-1-phosphate lyase 1) Enzyme-linked immunosorbent assay reagent |
| Alternative technique: |
elisas |
| Alternative to gene target: |
Cytoplasm, S1PL and SPL, SGPL1 and IDBG-629798 and ENSBTAG00000009984 and 522515, SGPL1 and IDBG-77509 and ENSG00000166224 and 8879, Sgpl1 and IDBG-155094 and ENSMUSG00000020097 and 20397, pyridoxal phosphate binding, this GO :0001553 and luteinization and biological process this GO :0001570 and vasculogenesis and biological process this GO :0001822 and kidney development and biological process this GO :0005515 and protein binding and molecular function this GO :0005783 and endoplasmic reticulum and cellular component this GO :0005789 and endoplasmic reticulum membrane and cellular component this GO :0006631 and fatty acid metabolic process and biological process this GO :0006665 and sphin this GO lipid metabolic process and biological process this GO :0006672 and ceramide metabolic process and biological process this GO :0006807 and nitrogen compound metabolic process and biological process this GO :0007283 and spermatogenesis and biological process this GO :0008117 and sphinganine-1-phosphate aldolase activity and molecular function this GO :0008152 and metabolic process and biological process this GO :0008209 and androgen metabolic process and biological process this GO :0008210 and estrogen metabolic process and biological process this GO :0008585 and female this GO nad development and biological process this GO :0009058 and biosynthetic process and biological process this GO :0009791 and post-embryonic development and biological process this GO :0010761 and fibroblast migration and biological process this GO :0016831 and carboxy-lyase activity and molecular function this GO :0019752 and carboxylic acid metabolic process and biological process this GO :0030097 and hemopoiesis and biological process this GO :0030148 and sphin this GO lipid biosynthetic process and biological process this GO :0030149 and sphin this GO lipid catabolic process and biological process this GO :0030170 and pyridoxal phosphate binding and molecular function this GO :0030176 and integral component of endoplasmic reticulum membrane and cellular component this GO :0033327 and Leydig cell differentiation and biological process this GO :0040014 and regulation of multicellular organism growth and biological process this GO :0044281 and small molecule metabolic process and biological process this GO :0048008 and platelet-derived growth factor receptor signaling pathway and biological process this GO :0048705 and skeletal system morphogenesis and biological process this GO :0060021 and palate development and biological process this GO :0060325 and face morphogenesis and biological process this GO :0097194 and execution phase of apoptosis and biological process, this GO :0005515 : protein binding, this GO :0005515 : protein binding and also this GO :0008117 : sphinganine-1-phosphate aldolase activity and also this GO :0016831 : carboxy-lyase activity and also this GO :0030170 : pyridoxal phosphate binding, this GO :0008117 : sphinganine-1-phosphate aldolase activity, this GO :0016831 : carboxy-lyase activity, this GO :0030170 : pyridoxal phosphate binding, sphin this GO sine-1-phosphate lyase 1 |
| Identity: |
10817 |
| Gene: |
SGPL1 |
More about : SGPL1 |
| Long gene name: |
sphingosine-1-phosphate lyase 1 |
| Synonyms: |
SPL |
| Locus: |
10q22, 1 |
| Discovery year: |
1999-02-03 |
| GenBank acession: |
AI128825 |
| Entrez gene record: |
8879 |
| Pubmed identfication: |
9464245 17090686 |
| RefSeq identity: |
NM_003901 |
| Havana BLAST/BLAT: |
OTTHUMG00000018421 |