| Antigen detectable with this ELISA kit: |
Prohibitin (PHB) |
| Species reactivity: |
Homo sapiens (Human) |
| Range of detection: |
0, 0, 1, 10ng/mL, 2, 5ng/mL, 25ng/mL, 313-20ng/mL The standard curve concentrations used for the ELISA&rsquo, 313ng/mL, 5ng/mL, 625ng/mL, s were 20ng/mL |
| Sensitivity: |
123ng/mL, The minimum detectable dose of this kit is typically less than 0 |
| Application: |
ELISA |
| Sample types tested for this ELISA kit: |
plasma and other biological fluids, Serum |
| Time for performing t hourse test: |
4, 5 hoursours |
| Test principle: |
10nm, After TMB substrate solution is added, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated, Next, Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Prohibitin (PHB), The concentration of Prohibitin (PHB) in the samples is then determined by comparing the O, The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm &, The microtiter plate provided in this kit has been pre-coated with an antibody specific to Prohibitin (PHB), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color, of the samples to the standard curve, only those wells that contain Prohibitin (PHB), D, The test principle applied in this kit is Sandwich enzyme immunoassay, plusmn |
| Storage and handling: |
Do not freeze any of the components of the ELISA kit, store all of the kit's components regrigerated at tempretaures between 2 and 6 degrees Celcius, Upon receipt |
| Advisory: |
Prior to use, briefly centrifuge these liquid components to collect all of the amount on the bottom of the vial, Due to transportation and handling issues small amounts of the liquid components of the ELISA kit may get trapped onto the seal or walls of the tubes |
| Properties: |
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays, E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays |
| Test: |
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED |
| Gene target: |
Prohibitin (PHB) |
| Short name: |
Prohibitin (PHB) ELISA kit |
| Technique: |
&hellip, A sign detected by the Colorimetric detection OD optical density absorbance at 450 nm between 0 and 4 with an ELISA reader for 450 nm absortion, Also Enzyme-linked immunoassay, ELISA test kits can be sandwich ELISA, ELISA tests are enzyme linked immunoassays to detect human, ELISAs Enzyme-linked immunosorbent assay 90320007 SNOMED code are used by the medical researcher for detection of human, Genprice Inc, In sandwich ELISAS Enzyme-linked immunosorbent assay 9we use captor and detector antibodies, In single antibody ELISAs the antigen is coated and only a detector antibody is used, In this case the ratio on your graph will be inverse proportional with the antigen present in your sample, MeSH code E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay, MeSH code E05 478 566 350 170 for Enzyme-Linked Immunosorbent Assay or ELISA kits, PROMT code 3841327 Immunoabsorbent assay tests, This is the desigation of other diagnostic analyzers and tests, Traditional competition antigen ELISAs are coated with a captor antibody and a competitive antigen is labelled with the chromogen, competitive have the SNOMED name 61461007 and the PROMT code for ELISA readers is 3841330, mouse, mouse or other proteins in serum, plasma, proteins are supplied in coated 96 well plates to be stored at +4°, the recognized authority tax ID 45-4304622 D-U-N-S number - 078440800, urine or biological fluids, C, ELISA kit |
| Host: |
antigen assay ELISA kit 1 Kit (96 Wells) |
| Alternative name: |
Prohibitin (prohibitin) Enzyme-linked immunosorbent assay reagent |
| Alternative technique: |
elisas |
| Alternative to gene target: |
DNA-templated and biological process this GO :0006366 and transcription from RNA polymerase II promoter and biological process this GO :0007165 and signal transduction and biological process this GO :0008285 and negative regulation of cell proliferation and biological process this GO :0010944 and negative regulation of transcription by competitive promoter binding and biological process this GO :0016020 and membrane and cellular component this GO :0016575 and histone deacetylation and biological process this GO :0019899 and enzyme binding and molecular function this GO :0030308 and negative regulation of cell growth and biological process this GO :0042826 and histone deacetylase binding and molecular function this GO :0042981 and regulation of apoptotic process and biological process this GO :0044212 and transcription regulatory region DNA binding and molecular function this GO :0045892 and negative regulation of transcription, DNA-templated and biological process this GO :0045893 and positive regulation of transcription, DNA-templated and biological process this GO :0050847 and progesterone receptor signaling pathway and biological process this GO :0060766 and negative regulation of androgen receptor signaling pathway and biological process this GO :0070062 and extracellular vesicular exosome and cellular component this GO :0071354 and cellular response to interleukin-6 and biological process this GO :0071897 and DNA biosynthetic process and biological process this GO :2000323 and negative regulation of glucocorticoid receptor signaling pathway and biological process, HEL-215 and HEL-S-54e and PHB1, PHB and IDBG-57598 and ENSG00000167085 and 5245, PHB and IDBG-638810 and ENSBTAG00000017120 and 530409, Phb and IDBG-209638 and ENSMUSG00000038845 and 18673, nuclei, this GO :0000122 and negative regulation of transcription from RNA polymerase II promoter and biological process this GO :0000981 and sequence-specific DNA binding RNA polymerase II transcription factor activity and molecular function this GO :0001649 and osteoblast differentiation and biological process this GO :0005515 and protein binding and molecular function this GO :0005634 and nucleus and cellular component this GO :0005654 and nucleoplasm and cellular component this GO :0005737 and cytoplasm and cellular component this GO :0005739 and mitochondrion and cellular component this GO :0005743 and mitochondrial inner membrane and cellular component this GO :0005887 and integral component of plasma membrane and cellular component this GO :0006260 and DNA replication and biological process this GO :0006355 and regulation of transcription, this GO :0000981 : sequence-specific DNA binding RNA polymerase II transcription factor activity, this GO :0000981 : sequence-specific DNA binding RNA polymerase II transcription factor activity and also this GO :0005515 : protein binding and also this GO :0019899 : enzyme binding and also this GO :0042826 : histone deacetylase binding and also this GO :0044212 : transcription regulatory region DNA binding, this GO :0005515 : protein binding, this GO :0019899 : enzyme binding, this GO :0042826 : histone deacetylase binding, this GO :0044212 : transcription regulatory region DNA binding, transcription regulatory region DNA binding, 237880, prohibitin |
| Identity: |
8912 |
| Gene: |
PHB |
More about : PHB |
| Long gene name: |
prohibitin |
| Synonyms: |
PHB1 |
| Locus: |
17q21, 33 |
| Discovery year: |
1991-03-04 |
| Entrez gene record: |
5245 |
| Pubmed identfication: |
10376528 8244394 |
| RefSeq identity: |
NM_002634 |
| Havana BLAST/BLAT: |
OTTHUMG00000134271 |