| Antigen detectable with this ELISA kit: |
cGMP Specific (PDE5A), Phosphodiesterase 5A |
| Species reactivity: |
Homo sapiens (Human) |
| Range of detection: |
1, 1, 3, 6, 12, 25ng/mL, 50ng/mL, 125ng/mL, 25ng/mL, 563-100ng/mL The standard curve concentrations used for the ELISA&rsquo, 563ng/mL, 5ng/mL, s were 100ng/mL |
| Sensitivity: |
56ng/mL, The minimum detectable dose of this kit is typically less than 0 |
| Application: |
ELISA |
| Sample types tested for this ELISA kit: |
plasma, tissue homogenates and other biological fluids, Serum |
| Time for performing t hourse test: |
4, 5 hoursours |
| Test principle: |
10nm, After TMB substrate solution is added, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated, Next, Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Phosphodiesterase 5A, The concentration of Phosphodiesterase 5A, The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm &, The microtiter plate provided in this kit has been pre-coated with an antibody specific to Phosphodiesterase 5A, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color, cGMP Specific (PDE5A), cGMP Specific (PDE5A), cGMP Specific (PDE5A), cGMP Specific (PDE5A) in the samples is then determined by comparing the O, of the samples to the standard curve, only those wells that contain Phosphodiesterase 5A, D, The test principle applied in this kit is Sandwich enzyme immunoassay, plusmn |
| Storage and handling: |
Do not freeze any of the components of the ELISA kit, store all of the kit's components regrigerated at tempretaures between 2 and 6 degrees Celcius, Upon receipt |
| Advisory: |
Prior to use, briefly centrifuge these liquid components to collect all of the amount on the bottom of the vial, Due to transportation and handling issues small amounts of the liquid components of the ELISA kit may get trapped onto the seal or walls of the tubes |
| Properties: |
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays, E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays |
| Test: |
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED |
| Gene target: |
cGMP Specific (PDE5A), Phosphodiesterase 5A |
| Short name: |
cGMP Specific (PDE5A) ELISA kit, Phosphodiesterase 5A |
| Technique: |
&hellip, A sign detected by the Colorimetric detection OD optical density absorbance at 450 nm between 0 and 4 with an ELISA reader for 450 nm absortion, Also Enzyme-linked immunoassay, ELISA test kits can be sandwich ELISA, ELISA tests are enzyme linked immunoassays to detect human, ELISAs Enzyme-linked immunosorbent assay 90320007 SNOMED code are used by the medical researcher for detection of human, Genprice Inc, In sandwich ELISAS Enzyme-linked immunosorbent assay 9we use captor and detector antibodies, In single antibody ELISAs the antigen is coated and only a detector antibody is used, In this case the ratio on your graph will be inverse proportional with the antigen present in your sample, MeSH code E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay, MeSH code E05 478 566 350 170 for Enzyme-Linked Immunosorbent Assay or ELISA kits, PROMT code 3841327 Immunoabsorbent assay tests, This is the desigation of other diagnostic analyzers and tests, Traditional competition antigen ELISAs are coated with a captor antibody and a competitive antigen is labelled with the chromogen, competitive have the SNOMED name 61461007 and the PROMT code for ELISA readers is 3841330, mouse, mouse or other proteins in serum, plasma, proteins are supplied in coated 96 well plates to be stored at +4°, the recognized authority tax ID 45-4304622 D-U-N-S number - 078440800, urine or biological fluids, C, ELISA kit |
| Host: |
antigen assay ELISA kit 1 Kit (96 Wells) |
| Alternative name: |
cGMP Specific (phosphodiesterase 5A, cGMP-specific) Enzyme-linked immunosorbent assay reagent, Phosphodiesterase 5A |
| Alternative technique: |
elisas |
| Alternative to gene target: |
3', CGB-PDE and CN5A and PDE5, Cytoplasm, PDE5A and IDBG-35773 and ENSG00000138735 and 8654, PDE5A and IDBG-639844 and ENSBTAG00000024888 and 281972, Pde5a and IDBG-191818 and ENSMUSG00000053965 and 242202, cGMP-specific, this GO :0004112 and cyclic-nucleotide phosphodiesterase activity and molecular function this GO :0004114 and 3', this GO :0004112 : cyclic-nucleotide phosphodiesterase activity, this GO :0004112 : cyclic-nucleotide phosphodiesterase activity and also this GO :0004114 : 3', this GO :0004114 : 3', this GO :0005515 : protein binding, this GO :0030553 : cGMP binding, this GO :0046872 : metal ion binding, this GO :0047555 : 3', 5'-cyclic-GMP phosphodiesterase activity, 5'-cyclic-GMP phosphodiesterase activity, 5'-cyclic-GMP phosphodiesterase activity and molecular function this GO :0055118 and negative regulation of cardiac muscle contraction and biological process this GO :0055119 and relaxation of cardiac muscle and biological process this GO :0060282 and positive regulation of oocyte development and biological process, 5'-cyclic-nucleotide phosphodiesterase activity, 5'-cyclic-nucleotide phosphodiesterase activity and also this GO :0005515 : protein binding and also this GO :0030553 : cGMP binding and also this GO :0046872 : metal ion binding and also this GO :0047555 : 3', 5'-cyclic-nucleotide phosphodiesterase activity and molecular function this GO :0005515 and protein binding and molecular function this GO :0005575 and cellular component and cellular component this GO :0005829 and cytosol and cellular component this GO :0007165 and signal transduction and biological process this GO :0007596 and blood coagulation and biological process this GO :0010613 and positive regulation of cardiac muscle hypertrophy and biological process this GO :0030553 and cGMP binding and molecular function this GO :0042130 and negative regulation of T cell proliferation and biological process this GO :0043406 and positive regulation of MAP kinase activity and biological process this GO :0046068 and cGMP metabolic process and biological process this GO :0046069 and cGMP catabolic process and biological process this GO :0046872 and metal ion binding and molecular function this GO :0047555 and 3', phosphodiesterase 5A |
| Identity: |
8784 |
| Gene: |
PDE5A |
More about : PDE5A |
| Long gene name: |
phosphodiesterase 5A |
| Synonyms gene name: |
cGMP-specific , phosphodiesterase 5A |
| Locus: |
4q26 |
| Discovery year: |
1998-11-24 |
| GenBank acession: |
D89094 |
| Entrez gene record: |
8654 |
| Pubmed identfication: |
9714779 9642111 |
| RefSeq identity: |
NM_001083 |
| Classification: |
Phosphodiesterases |
| Havana BLAST/BLAT: |
OTTHUMG00000132971 |