| Antigen detectable with this ELISA kit: |
Perforin 1 (PRF1) |
| Species reactivity: |
Mus musculus (Mouse) |
| Range of detection: |
0, 0, 1, 10ng/mL, 2, 5ng/mL, 25ng/mL, 313-20ng/mL The standard curve concentrations used for the ELISA&rsquo, 313ng/mL, 5ng/mL, 625ng/mL, s were 20ng/mL |
| Sensitivity: |
119ng/mL, The minimum detectable dose of this kit is typically less than 0 |
| Application: |
ELISA |
| Sample types tested for this ELISA kit: |
cell culture supernates and other biological fluids, cell lysates, plasma, tissue homogenates, Serum |
| Time for performing t hourse test: |
4, 5 hoursours |
| Test principle: |
10nm, After TMB substrate solution is added, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated, Next, Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Perforin 1 (PRF1), The concentration of Perforin 1 (PRF1) in the samples is then determined by comparing the O, The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm &, The microtiter plate provided in this kit has been pre-coated with an antibody specific to Perforin 1 (PRF1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color, of the samples to the standard curve, only those wells that contain Perforin 1 (PRF1), D, The test principle applied in this kit is Sandwich enzyme immunoassay, plusmn |
| Storage and handling: |
Do not freeze any of the components of the ELISA kit, store all of the kit's components regrigerated at tempretaures between 2 and 6 degrees Celcius, Upon receipt |
| Advisory: |
Prior to use, briefly centrifuge these liquid components to collect all of the amount on the bottom of the vial, Due to transportation and handling issues small amounts of the liquid components of the ELISA kit may get trapped onto the seal or walls of the tubes |
| Properties: |
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays, E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays |
| Test: |
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED |
| Gene target: |
Perforin 1 (PRF1) |
| Short name: |
Perforin 1 (PRF1) ELISA kit |
| Technique: |
&hellip, A sign detected by the Colorimetric detection OD optical density absorbance at 450 nm between 0 and 4 with an ELISA reader for 450 nm absortion, Also Enzyme-linked immunoassay, ELISA test kits can be sandwich ELISA, ELISA tests are enzyme linked immunoassays to detect human, ELISAs Enzyme-linked immunosorbent assay 90320007 SNOMED code are used by the medical researcher for detection of human, Genprice Inc, In sandwich ELISAS Enzyme-linked immunosorbent assay 9we use captor and detector antibodies, In single antibody ELISAs the antigen is coated and only a detector antibody is used, In this case the ratio on your graph will be inverse proportional with the antigen present in your sample, MeSH code E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay, MeSH code E05 478 566 350 170 for Enzyme-Linked Immunosorbent Assay or ELISA kits, PROMT code 3841327 Immunoabsorbent assay tests, This is the desigation of other diagnostic analyzers and tests, Traditional competition antigen ELISAs are coated with a captor antibody and a competitive antigen is labelled with the chromogen, competitive have the SNOMED name 61461007 and the PROMT code for ELISA readers is 3841330, mouse, mouse or other proteins in serum, plasma, proteins are supplied in coated 96 well plates to be stored at +4°, the recognized authority tax ID 45-4304622 D-U-N-S number - 078440800, urine or biological fluids, C, ELISA kit |
| Host: |
antigen assay ELISA kit 1 Kit (96 Wells) |
| Alternative name: |
Perforin 1 (perforin 1 (pore forming protein)) Enzyme-linked immunosorbent assay reagent |
| Alternative technique: |
elisas |
| Alternative to gene target: |
BT, Extracellular, FLH2 and HPLH2 and P1 and PFN1 and PFP, PRF1 and IDBG-77451 and ENSG00000180644 and 5551, Prf1 and IDBG-155739 and ENSMUSG00000037202 and 18646, this GO :0002357 and defense response to tumor cell and biological process this GO :0002418 and immune response to tumor cell and biological process this GO :0005509 and calcium ion binding and molecular function this GO :0005515 and protein binding and molecular function this GO :0005576 and extracellular region and cellular component this GO :0005886 and plasma membrane and cellular component this GO :0006915 and apoptotic process and biological process this GO :0006968 and cellular defense response and biological process this GO :0016020 and membrane and cellular component this GO :0016021 and integral component of membrane and cellular component this GO :0016023 and cytoplasmic membrane-bounded vesicle and cellular component this GO :0019835 and cytolysis and biological process this GO :0022829 and wide pore channel activity and molecular function this GO :0031904 and endosome lumen and cellular component this GO :0044194 and cytolytic granule and cellular component this GO :0051260 and protein homooli this GO merization and biological process this GO :0051607 and defense response to virus and biological process this GO :0055085 and transmembrane transport and biological process, this GO :0005509 : calcium ion binding, this GO :0005509 : calcium ion binding and also this GO :0005515 : protein binding and also this GO :0022829 : wide pore channel activity, this GO :0005515 : protein binding, this GO :0022829 : wide pore channel activity, wide pore channel activity, 21975 and IDBG-629772 and ENSBTAG00000001008 and 369025, perforin 1 (pore forming protein) |
| Identity: |
9360 |
| Gene: |
PRF1 |
More about : PRF1 |
| Long gene name: |
perforin 1 |
| Synonyms gene name: |
perforin 1 (pore forming protein) |
| Synonyms: |
PFP P1 HPLH2 |
| Synonyms name: |
Perforin perforin 1 (preforming protein) |
| Locus: |
10q22, 1 |
| Discovery year: |
1989-02-23 |
| GenBank acession: |
BC047695 |
| Entrez gene record: |
5551 |
| Pubmed identfication: |
1505959 2592021 |
| RefSeq identity: |
NM_005041 |
| Classification: |
C2 domain containing |
| Havana BLAST/BLAT: |
OTTHUMG00000018412 |
| Locus Specific Databases: |
PRF1base: Mutation registry for Familiar haemophagocytic lymphohistiocytosis, type II (FHL2) LRG_94 |