| Antigen detectable with this ELISA kit: |
Neutrophil Cytosolic Factor 1 (NCF1) |
| Species reactivity: |
Homo sapiens (Human) |
| Range of detection: |
0, 0, 1, 10ng/mL, 2, 5ng/mL, 25ng/mL, 313-20ng/mL The standard curve concentrations used for the ELISA&rsquo, 313ng/mL, 5ng/mL, 625ng/mL, s were 20ng/mL |
| Sensitivity: |
117ng/mL, The minimum detectable dose of this kit is typically less than 0 |
| Application: |
ELISA |
| Sample types tested for this ELISA kit: |
cell lysates and other biological fluids, Tissue homogenates |
| Time for performing t hourse test: |
4, 5 hoursours |
| Test principle: |
10nm, After TMB substrate solution is added, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated, Next, Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Neutrophil Cytosolic Factor 1 (NCF1), The concentration of Neutrophil Cytosolic Factor 1 (NCF1) in the samples is then determined by comparing the O, The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm &, The microtiter plate provided in this kit has been pre-coated with an antibody specific to Neutrophil Cytosolic Factor 1 (NCF1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color, of the samples to the standard curve, only those wells that contain Neutrophil Cytosolic Factor 1 (NCF1), D, The test principle applied in this kit is Sandwich enzyme immunoassay, plusmn |
| Storage and handling: |
Do not freeze any of the components of the ELISA kit, store all of the kit's components regrigerated at tempretaures between 2 and 6 degrees Celcius, Upon receipt |
| Advisory: |
Prior to use, briefly centrifuge these liquid components to collect all of the amount on the bottom of the vial, Due to transportation and handling issues small amounts of the liquid components of the ELISA kit may get trapped onto the seal or walls of the tubes |
| Properties: |
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays, E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays |
| Test: |
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED |
| Gene target: |
Neutrophil Cytosolic Factor 1 (NCF1) |
| Short name: |
Neutrophil Cytosolic Factor 1 (NCF1) ELISA kit |
| Technique: |
&hellip, A sign detected by the Colorimetric detection OD optical density absorbance at 450 nm between 0 and 4 with an ELISA reader for 450 nm absortion, Also Enzyme-linked immunoassay, ELISA test kits can be sandwich ELISA, ELISA tests are enzyme linked immunoassays to detect human, ELISAs Enzyme-linked immunosorbent assay 90320007 SNOMED code are used by the medical researcher for detection of human, Genprice Inc, In sandwich ELISAS Enzyme-linked immunosorbent assay 9we use captor and detector antibodies, In single antibody ELISAs the antigen is coated and only a detector antibody is used, In this case the ratio on your graph will be inverse proportional with the antigen present in your sample, MeSH code E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay, MeSH code E05 478 566 350 170 for Enzyme-Linked Immunosorbent Assay or ELISA kits, PROMT code 3841327 Immunoabsorbent assay tests, This is the desigation of other diagnostic analyzers and tests, Traditional competition antigen ELISAs are coated with a captor antibody and a competitive antigen is labelled with the chromogen, competitive have the SNOMED name 61461007 and the PROMT code for ELISA readers is 3841330, mouse, mouse or other proteins in serum, plasma, proteins are supplied in coated 96 well plates to be stored at +4°, the recognized authority tax ID 45-4304622 D-U-N-S number - 078440800, urine or biological fluids, C, ELISA kit |
| Host: |
antigen assay ELISA kit 1 Kit (96 Wells) |
| Alternative name: |
Neutrophil Cytosolic Factor 1 (neutrophil cytosolic factor 1) Enzyme-linked immunosorbent assay reagent |
| Alternative technique: |
elisas |
| Alternative to gene target: |
NCF1 and IDBG-21570 and ENSG00000158517 and 653361, NCF1 and IDBG-639883 and ENSBTAG00000003305 and 281345, Ncf1 and IDBG-202592 and ENSMUSG00000015950 and 17969, Plasma membranes, TAP-dependent and biological process this GO :0002679 and respiratory burst involved in defense response and biological process this GO :0005515 and protein binding and molecular function this GO :0005737 and cytoplasm and cellular component this GO :0005791 and rough endoplasmic reticulum and cellular component this GO :0005794 and this GO lgi apparatus and cellular component this GO :0005829 and cytosol and cellular component this GO :0005886 and plasma membrane and cellular component this GO :0006612 and protein targeting to membrane and biological process this GO :0006691 and leukotriene metabolic process and biological process this GO :0006801 and superoxide metabolic process and biological process this GO :0006915 and apoptotic process and biological process this GO :0006954 and inflammatory response and biological process this GO :0006968 and cellular defense response and biological process this GO :0008283 and cell proliferation and biological process this GO :0009055 and electron carrier activity and molecular function this GO :0009617 and response to bacterium and biological process this GO :0016020 and membrane and cellular component this GO :0016175 and superoxide-generating NADPH oxidase activity and molecular function this GO :0017124 and SH3 domain binding and molecular function this GO :0019898 and extrinsic component of membrane and cellular component this GO :0030425 and dendrite and cellular component this GO :0032010 and pha this GO lysosome and cellular component this GO :0035091 and phosphatidylinositol binding and molecular function this GO :0042554 and superoxide anion generation and biological process this GO :0042590 and antigen processing and presentation of exogenous peptide antigen via MHC class I and biological process this GO :0042742 and defense response to bacterium and biological process this GO :0043020 and NADPH oxidase complex and cellular component this GO :0043025 and neuronal cell body and cellular component this GO :0043325 and phosphatidylinositol-3, phosphatidylinositol-3, this GO :0001878 and response to yeast and biological process this GO :0001909 and leukocyte mediated cytotoxicity and biological process this GO :0002474 and antigen processing and presentation of peptide antigen via MHC class I and biological process this GO :0002479 and antigen processing and presentation of exogenous peptide antigen via MHC class I, this GO :0005515 : protein binding, this GO :0005515 : protein binding and also this GO :0009055 : electron carrier activity and also this GO :0016175 : superoxide-generating NADPH oxidase activity and also this GO :0017124 : SH3 domain binding and also this GO :0035091 : phosphatidylinositol binding and also this GO :0043325 : phosphatidylinositol-3, this GO :0009055 : electron carrier activity, this GO :0016175 : superoxide-generating NADPH oxidase activity, this GO :0017124 : SH3 domain binding, this GO :0035091 : phosphatidylinositol binding, this GO :0043325 : phosphatidylinositol-3, 4-bisphosphate binding, 4-bisphosphate binding, 4-bisphosphate binding and molecular function this GO :0045087 and innate immune response and biological process this GO :0045730 and respiratory burst and biological process this GO :0045986 and negative regulation of smooth muscle contraction and biological process this GO :0050665 and hydrogen peroxide biosynthetic process and biological process this GO :0050830 and defense response to Gram-positive bacterium and biological process this GO :0050832 and defense response to fungus and biological process this GO :0051701 and interaction with host and biological process this GO :0055114 and oxidation-reduction process and biological process this GO :0070946 and neutrophil mediated killing of gram-positive bacterium and biological process this GO :0070947 and neutrophil mediated killing of fungus and biological process this GO :0090382 and pha this GO some maturation and biological process, neutrophil cytosolic factor 1 |
| Identity: |
7660 |
| Gene: |
NCF1 |
More about : NCF1 |
| Long gene name: |
neutrophil cytosolic factor 1 |
| Synonyms gene name: |
autosomal 1) , chronic granulomatous disease, neutrophil cytosolic factor 1 (47kD |
| Synonyms: |
p47phox NOXO2 NCF1A SH3PXD1A |
| Synonyms name: |
NADPH oxidase organizer 2 chronic granulomatous disease, autosomal 1 |
| Locus: |
7q11, 23 |
| Discovery year: |
1989-05-25 |
| GenBank acession: |
M25665 |
| Entrez gene record: |
653361 |
| RefSeq identity: |
NM_000265 |
| Havana BLAST/BLAT: |
OTTHUMG00000149965 |
| Locus Specific Databases: |
NCF1base: Mutation registry for autosomal recessive chronic granulomatous disease (CGD), deficiency of p47phox LRG_87 |