| Antigen detectable with this ELISA kit: |
Interleukin 4 (IL4) |
| Species reactivity: |
Bovine (Cattle), Bos taurus |
| Range of detection: |
125pg/mL, 15, 250pg/mL, 31, 500pg/mL, 15, 62, 25pg/mL, 5pg/mL, 625-1000pg/mL The standard curve concentrations used for the ELISA&rsquo, 625pg/mL, s were 1000pg/mL |
| Sensitivity: |
5pg/mL, The minimum detectable dose of this kit is typically less than 6 |
| Application: |
ELISA |
| Sample types tested for this ELISA kit: |
cell culture supernates and other biological fluids, cell lysates, plasma, tissue homogenates, Serum |
| Time for performing t hourse test: |
4, 5 hoursours |
| Test principle: |
10nm, After TMB substrate solution is added, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated, Next, Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Interleukin 4 (IL4), The concentration of Interleukin 4 (IL4) in the samples is then determined by comparing the O, The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm &, The microtiter plate provided in this kit has been pre-coated with an antibody specific to Interleukin 4 (IL4), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color, of the samples to the standard curve, only those wells that contain Interleukin 4 (IL4), D, The test principle applied in this kit is Sandwich enzyme immunoassay, plusmn |
| Storage and handling: |
Do not freeze any of the components of the ELISA kit, store all of the kit's components regrigerated at tempretaures between 2 and 6 degrees Celcius, Upon receipt |
| Advisory: |
Prior to use, briefly centrifuge these liquid components to collect all of the amount on the bottom of the vial, Due to transportation and handling issues small amounts of the liquid components of the ELISA kit may get trapped onto the seal or walls of the tubes |
| Properties: |
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays, E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays |
| Test: |
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED |
| Gene: |
IL4 |
More about : IL4 |
| Gene target: |
Interleukin 4 (IL4) |
| Short name: |
Interleukin 4 (IL4) ELISA kit |
| Technique: |
&hellip, A sign detected by the Colorimetric detection OD optical density absorbance at 450 nm between 0 and 4 with an ELISA reader for 450 nm absortion, Also Enzyme-linked immunoassay, ELISA test kits can be sandwich ELISA, ELISA tests are enzyme linked immunoassays to detect human, ELISAs Enzyme-linked immunosorbent assay 90320007 SNOMED code are used by the medical researcher for detection of human, Genprice Inc, In sandwich ELISAS Enzyme-linked immunosorbent assay 9we use captor and detector antibodies, In single antibody ELISAs the antigen is coated and only a detector antibody is used, In this case the ratio on your graph will be inverse proportional with the antigen present in your sample, MeSH code E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay, MeSH code E05 478 566 350 170 for Enzyme-Linked Immunosorbent Assay or ELISA kits, PROMT code 3841327 Immunoabsorbent assay tests, This is the desigation of other diagnostic analyzers and tests, Traditional competition antigen ELISAs are coated with a captor antibody and a competitive antigen is labelled with the chromogen, competitive have the SNOMED name 61461007 and the PROMT code for ELISA readers is 3841330, mouse, mouse or other proteins in serum, plasma, proteins are supplied in coated 96 well plates to be stored at +4°, the recognized authority tax ID 45-4304622 D-U-N-S number - 078440800, urine or biological fluids, C, ELISA kit |
| Host: |
antigen assay ELISA kit 1 Kit (96 Wells) |
| Alternative name: |
Interleukin 4 (interleukin 4) Enzyme-linked immunosorbent assay reagent |
| Alternative technique: |
elisas |
| Alternative to gene target: |
BCGF-1 and BCGF1 and BSF-1 and BSF1 and IL-4, DNA-templated and biological process this GO :0045893 and positive regulation of transcription, DNA-templated and biological process this GO :0045944 and positive regulation of transcription from RNA polymerase II promoter and biological process this GO :0048295 and positive regulation of isotype switching to IgE isotypes and biological process this GO :0048304 and positive regulation of isotype switching to IgG isotypes and biological process this GO :0050727 and regulation of inflammatory response and biological process this GO :0050731 and positive regulation of peptidyl-tyrosine phosphorylation and biological process this GO :0050776 and regulation of immune response and biological process this GO :0050868 and negative regulation of T cell activation and biological process this GO :0050871 and positive regulation of B cell activation and biological process this GO :0051091 and positive regulation of sequence-specific DNA binding transcription factor activity and biological process this GO :0060041 and retina development in camera-type eye and biological process this GO :0071288 and cellular response to mercury ion and biological process this GO :0071677 and positive regulation of mononuclear cell migration and biological process this GO :0097028 and dendritic cell differentiation and biological process this GO :0097192 and extrinsic apoptotic signaling pathway in absence of ligand and biological process this GO :2000320 and negative regulation of T-helper 17 cell differentiation and biological process this GO :2000424 and positive regulation of eosinophil chemotaxis and biological process this GO :2001237 and negative regulation of extrinsic apoptotic signaling pathway and biological process, Extracellular, IL4 and IDBG-42701 and ENSG00000113520 and 3565, IL4 and IDBG-644671 and ENSBTAG00000015957 and 280824, Il4 and IDBG-172024 and ENSMUSG00000000869 and 16189, growth factor activity, this GO :0001774 and microglial cell activation and biological process this GO :0001934 and positive regulation of protein phosphorylation and biological process this GO :0002227 and innate immune response in mucosa and biological process this GO :0002230 and positive regulation of defense response to virus by host and biological process this GO :0002296 and T-helper 1 cell lineage commitment and biological process this GO :0002674 and negative regulation of acute inflammatory response and biological process this GO :0002677 and negative regulation of chronic inflammatory response and biological process this GO :0005125 and cytokine activity and molecular function this GO :0005126 and cytokine receptor binding and molecular function this GO :0005136 and interleukin-4 receptor binding and molecular function this GO :0005515 and protein binding and molecular function this GO :0005576 and extracellular region and cellular component this GO :0005615 and extracellular space and cellular component this GO :0006935 and chemotaxis and biological process this GO :0006955 and immune response and biological process this GO :0006968 and cellular defense response and biological process this GO :0007565 and female pregnancy and biological process this GO :0007584 and response to nutrient and biological process this GO :0008083 and growth factor activity and molecular function this GO :0008203 and cholesterol metabolic process and biological process this GO :0008284 and positive regulation of cell proliferation and biological process this GO :0009897 and external side of plasma membrane and cellular component this GO :0010155 and regulation of proton transport and biological process this GO :0014070 and response to organic cyclic compound and biological process this GO :0030183 and B cell differentiation and biological process this GO :0030890 and positive regulation of B cell proliferation and biological process this GO :0031296 and B cell costimulation and biological process this GO :0032733 and positive regulation of interleukin-10 production and biological process this GO :0032736 and positive regulation of interleukin-13 production and biological process this GO :0034097 and response to cytokine and biological process this GO :0035745 and T-helper 2 cell cytokine production and biological process this GO :0042092 and type 2 immune response and biological process this GO :0042102 and positive regulation of T cell proliferation and biological process this GO :0042104 and positive regulation of activated T cell proliferation and biological process this GO :0042113 and B cell activation and biological process this GO :0042325 and regulation of phosphorylation and biological process this GO :0042493 and response to drug and biological process this GO :0042523 and positive regulation of tyrosine phosphorylation of Stat5 protein and biological process this GO :0042832 and defense response to protozoan and biological process this GO :0043031 and negative regulation of macrophage activation and biological process this GO :0043066 and negative regulation of apoptotic process and biological process this GO :0045019 and negative regulation of nitric oxide biosynthetic process and biological process this GO :0045064 and T-helper 2 cell differentiation and biological process this GO :0045080 and positive regulation of chemokine biosynthetic process and biological process this GO :0045087 and innate immune response and biological process this GO :0045189 and connective tissue growth factor biosynthetic process and biological process this GO :0045191 and regulation of isotype switching and biological process this GO :0045348 and positive regulation of MHC class II biosynthetic process and biological process this GO :0045471 and response to ethanol and biological process this GO :0045582 and positive regulation of T cell differentiation and biological process this GO :0045671 and negative regulation of osteoclast differentiation and biological process this GO :0045892 and negative regulation of transcription, this GO :0005125 : cytokine activity, this GO :0005125 : cytokine activity and also this GO :0005126 : cytokine receptor binding and also this GO :0005136 : interleukin-4 receptor binding and also this GO :0005515 : protein binding and also this GO :0008083 : growth factor activity, this GO :0005126 : cytokine receptor binding, this GO :0005136 : interleukin-4 receptor binding, this GO :0005515 : protein binding, this GO :0008083 : growth factor activity, interleukin 4 |
| Identity: |
6014 |
| Long gene name: |
interleukin 4 |
| Synonyms: |
BSF1 IL-4 BCGF1 BCGF-1 MGC79402 |
| Synonyms name: |
B_cell stimulatory factor 1 lymphocyte stimulatory factor 1 B cell growth factor 1 |
| Locus: |
5q31, 1 |
| Discovery year: |
1988-08-10 |
| GenBank acession: |
M23442 |
| Entrez gene record: |
3565 |
| Pubmed identfication: |
3016727 |
| RefSeq identity: |
NM_000589 |
| Classification: |
Interleukins |
| Havana BLAST/BLAT: |
OTTHUMG00000059724 |