| Antigen detectable with this ELISA kit: |
Granzyme A (GZMA) |
| Species reactivity: |
Mus musculus (Mouse) |
| Range of detection: |
0, 0, 0, 1, 2, 5ng/mL, 156-10ng/mL The standard curve concentrations used for the ELISA&rsquo, 156ng/mL, 25ng/mL, 313ng/mL, 5ng/mL, 625ng/mL, s were 10ng/mL |
| Sensitivity: |
058ng/mL, The minimum detectable dose of this kit is typically less than 0 |
| Application: |
ELISA |
| Sample types tested for this ELISA kit: |
cell lysates and other biological fluids, plasma, tissue homogenates, Serum |
| Time for performing t hourse test: |
4, 5 hoursours |
| Test principle: |
10nm, After TMB substrate solution is added, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated, Next, Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Granzyme A (GZMA), The concentration of Granzyme A (GZMA) in the samples is then determined by comparing the O, The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm &, The microtiter plate provided in this kit has been pre-coated with an antibody specific to Granzyme A (GZMA), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color, of the samples to the standard curve, only those wells that contain Granzyme A (GZMA), D, The test principle applied in this kit is Sandwich enzyme immunoassay, plusmn |
| Storage and handling: |
Do not freeze any of the components of the ELISA kit, store all of the kit's components regrigerated at tempretaures between 2 and 6 degrees Celcius, Upon receipt |
| Advisory: |
Prior to use, briefly centrifuge these liquid components to collect all of the amount on the bottom of the vial, Due to transportation and handling issues small amounts of the liquid components of the ELISA kit may get trapped onto the seal or walls of the tubes |
| Properties: |
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays, E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays |
| Test: |
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED |
| Gene target: |
Granzyme A (GZMA) |
| Short name: |
Granzyme A (GZMA) ELISA kit |
| Technique: |
&hellip, A sign detected by the Colorimetric detection OD optical density absorbance at 450 nm between 0 and 4 with an ELISA reader for 450 nm absortion, Also Enzyme-linked immunoassay, ELISA test kits can be sandwich ELISA, ELISA tests are enzyme linked immunoassays to detect human, ELISAs Enzyme-linked immunosorbent assay 90320007 SNOMED code are used by the medical researcher for detection of human, Genprice Inc, In sandwich ELISAS Enzyme-linked immunosorbent assay 9we use captor and detector antibodies, In single antibody ELISAs the antigen is coated and only a detector antibody is used, In this case the ratio on your graph will be inverse proportional with the antigen present in your sample, MeSH code E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay, MeSH code E05 478 566 350 170 for Enzyme-Linked Immunosorbent Assay or ELISA kits, PROMT code 3841327 Immunoabsorbent assay tests, This is the desigation of other diagnostic analyzers and tests, Traditional competition antigen ELISAs are coated with a captor antibody and a competitive antigen is labelled with the chromogen, competitive have the SNOMED name 61461007 and the PROMT code for ELISA readers is 3841330, mouse, mouse or other proteins in serum, plasma, proteins are supplied in coated 96 well plates to be stored at +4°, the recognized authority tax ID 45-4304622 D-U-N-S number - 078440800, urine or biological fluids, C, ELISA kit |
| Host: |
antigen assay ELISA kit 1 Kit (96 Wells) |
| Alternative name: |
cytotoxic T-lymphocyte-associated serine esterase 3)) Enzyme-linked immunosorbent assay reagent, Granzyme A (granzyme A (granzyme 1 |
| Alternative technique: |
elisas |
| Alternative to gene target: |
BT, CTLA3 and HFSP, GZMA and IDBG-20850 and ENSG00000145649 and 3001, Gzma and IDBG-183453 and ENSMUSG00000023132 and 14938, cytotoxic T-lymphocyte-associated serine esterase 3), nuclei, protein homodimerization activity, this GO :0001772 and immunological synapse and cellular component this GO :0003824 and catalytic activity and molecular function this GO :0004252 and serine-type endopeptidase activity and molecular function this GO :0005515 and protein binding and molecular function this GO :0005576 and extracellular region and cellular component this GO :0005634 and nucleus and cellular component this GO :0006508 and proteolysis and biological process this GO :0006915 and apoptotic process and biological process this GO :0006955 and immune response and biological process this GO :0008236 and serine-type peptidase activity and molecular function this GO :0019835 and cytolysis and biological process this GO :0032078 and negative regulation of endodeoxyribonuclease activity and biological process this GO :0042803 and protein homodimerization activity and molecular function this GO :0043065 and positive regulation of apoptotic process and biological process this GO :0043392 and negative regulation of DNA binding and biological process this GO :0051354 and negative regulation of oxidoreductase activity and biological process this GO :0051603 and proteolysis involved in cellular protein catabolic process and biological process, this GO :0003824 : catalytic activity, this GO :0003824 : catalytic activity and also this GO :0004252 : serine-type endopeptidase activity and also this GO :0005515 : protein binding and also this GO :0008236 : serine-type peptidase activity and also this GO :0042803 : protein homodimerization activity, this GO :0004252 : serine-type endopeptidase activity, this GO :0005515 : protein binding, this GO :0008236 : serine-type peptidase activity, this GO :0042803 : protein homodimerization activity, 58970 and IDBG-629315 and ENSBTAG00000021958 and 539093, granzyme A (granzyme 1 |
| Identity: |
4708 |
| Gene: |
GZMA |
More about : GZMA |
| Long gene name: |
granzyme A |
| Synonyms gene: |
HFSP CTLA3 |
| Synonyms gene name: |
cytotoxic T-lymphocyte-associated serine esterase 3) , granzyme A (granzyme 1 |
| Synonyms name: |
CTL tryptase Cytotoxic T-lymphocyte-associated serine esterase-3 Hanukah factor serine protease) granzyme 1 |
| Locus: |
5q11, 2 |
| Discovery year: |
1988-05-31 |
| Entrez gene record: |
3001 |
| Pubmed identfication: |
3262682 3533635 |
| RefSeq identity: |
NM_006144 |
| Havana BLAST/BLAT: |
OTTHUMG00000097011 |