| Type: |
Conjugated Primary Antibody |
| Conjugated with: |
594, ALEXA FLUOR® |
| Host organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Protein/Peptide: |
Rap2A |
| Specificity: |
This antibody reacts specifically with Rap2A |
| Modification: |
No modification has been applied to this antibody |
| Modification site: |
None |
| Clonality: |
Polyclonal Antibody |
| Clone: |
Polyclonal Antibodies |
| Concentration: |
1ug per 1ul |
| Antigen Source: |
KLH conjugated synthetic peptide derived from human Rap2A |
| Gene ID: |
5911 |
| Applications: |
IF(IHC-P) |
| Applications with corresponding dilutions: |
IF(IHC-P)(1:50-200) |
| Cross reactive species: |
Mouse (Mus musculus), Rat (Rattus norvegicus), Human (Homo sapiens) |
| Cross Reactive Species details: |
However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species |
| Background information: |
In contrast with Rap 1A and Rap 1B, K-Ras and N-Ras proteins, Rap 1A is thought to interfere with Ras effector function by binding to Ras GAP in a GTP-dependent manner without affecting Rap 1A GTPase activity, Rap 2, The putative â, Two Ras-related human genes, another Ras-related protein, designated RAP1A and RAP1B, encode 95% homologous proteins (namely Rap 1A and Rap 1B) that share a similar C-terminal Cys-Ali-Ali-Xaa sequence with Ras proteins and are ubiquitously expressed in mammalian tissues, is conserved in both Rap 1 proteins, overexpression of Rap 2 does not interfere with the Ras signaling pathway, shares 60% identity with Rap 1A and exhibits a carboxy terminal CAAX motif and two upstream cysteines similar to those of the H-Ras, whose integrity is required for cell transformation as well as interaction with the putative effector protein GAP, &euro, &euro, &oelig, Ras oncogenes encode GTP-binding proteins that are capable of transforming immortalized cells in culture, effectorâ, domain of Ras proteins |
| Purification method: |
Purified by Protein A |
| Storage: |
50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA |
| Excitation emission: |
590nm/617nm |
| Synonyms: |
K-REV |
| Also known as: |
Rap2A Polyclonal Antibody |
| Other name: |
Anti-Rap2A Polyclonal |
| Advisory: |
For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody |
| Properties: |
For facs or microscopy Alexa 1 conjugate |
| Conjugation: |
Alexa Fluor |
| Group: |
Polyclonals and antibodies |
| About: |
The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera, immunohistochemistry on frozen slices or parrafin fixed tissues, Polyclonals can be used for Western blot |
| Gene target: |
Rap2A |
| Short name: |
Anti-Rap2A (Polyclonal) Fluor 594 |
| Technique: |
Pabs are a collection of immunoglobulin , Polyclonal antibodies , each identifying a different , whereas , (pAbs) are mostly rabbit or goat , B cells, Polyclonal, antibodies , antigen, come from a single N cell lineage, epitope, molecules that react against a specific , monoclonal antibodies , that are secreted by different |
| Label: |
ALEXA |
| Alternative name: |
ALEXA Fluor 594, antibody to-Rap2A (polyclonal) |
| Alternative technique: |
polyclonals |
| Identity: |
9861 |
| Gene: |
RAP2A |
More about : RAP2A |
| Long gene name: |
RAP2A, member of RAS oncogene family |
| Synonyms gene: |
RAP2 |
| Locus: |
13q32, 1 |
| Discovery year: |
1989-06-30 |
| GenBank acession: |
AF205602 |
| Entrez gene record: |
5911 |
| Classification: |
RAS type GTPase family |
| Havana BLAST/BLAT: |
OTTHUMG00000017240 |