| Type: |
Conjugated Primary Antibody |
| Conjugated with: |
594, ALEXA FLUOR® |
| Host organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Protein/Peptide: |
I35 |
| Specificity: |
This antibody reacts specifically with IFI35 |
| Modification: |
No modification has been applied to this antibody |
| Modification site: |
None |
| Clonality: |
Polyclonal Antibody |
| Clone: |
Polyclonal Antibodies |
| Concentration: |
1ug per 1ul |
| Antigen Source: |
KLH conjugated synthetic peptide derived from human IFI35 |
| Gene ID: |
3430 |
| Applications: |
IF(IHC-P) |
| Applications with corresponding dilutions: |
IF(IHC-P)(1:50-200) |
| Cross reactive species: |
Mouse (Mus musculus), Rat (Rattus norvegicus), Human (Homo sapiens) |
| Cross Reactive Species details: |
However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species |
| Background information: |
IFI-35 (Interferon-induced 35 kDa protein), IFI-35 is a leucine zipper protein that can form homodimers, IFI-35 is unable to be degraded by the proteasome, Localized to the nucleus and expressed in macrophages, Once complexed with Nmi, Upon induction by IFN-? IFI-35 associates with Nmi (N-Myc-interacting protein), also known as IFP35, but, cannot bind DNA, fibroblasts and epithelial cells, is a 286 amino acid interferon-induced protein, resulting in the formation of a high molecular weight complex that is thought to play a role in IFN-?signaling and cellular responses, suggesting that IFI-35 is protected from degradation only when needed by IFN-? Two isoforms of IFI-35 exist due to alternative splicing events, thereby controlling various events within the cell, unlike most leucine zipper proteins, The Interferon family of proteins are able to alter the expression of a variety of target genes |
| Purification method: |
Purified by Protein A |
| Storage: |
50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA |
| Excitation emission: |
590nm/617nm |
| Synonyms: |
IFP35 |
| Also known as: |
IFI35 Polyclonal Antibody |
| Other name: |
Anti-IFI35 Polyclonal |
| Advisory: |
For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody |
| Properties: |
For facs or microscopy Alexa 1 conjugate |
| Conjugation: |
Alexa Fluor |
| Group: |
Polyclonals and antibodies |
| About: |
The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera, immunohistochemistry on frozen slices or parrafin fixed tissues, Polyclonals can be used for Western blot |
| Gene target: |
IFI35 |
| Short name: |
Anti-IFI35 (Polyclonal) Fluor 594 |
| Technique: |
Pabs are a collection of immunoglobulin , Polyclonal antibodies , each identifying a different , whereas , (pAbs) are mostly rabbit or goat , B cells, Polyclonal, antibodies , antigen, come from a single N cell lineage, epitope, molecules that react against a specific , monoclonal antibodies , that are secreted by different |
| Label: |
ALEXA |
| Alternative name: |
ALEXA Fluor 594, antibody to-interferon-induced protein 35 (polyclonal) |
| Alternative technique: |
polyclonals |
| Alternative to gene target: |
IFI35 and IDBG-52075 and ENSG00000068079 and 3430, IFI35 and IDBG-640619 and ENSBTAG00000007389 and 510697, IFP35, Ifi35 and IDBG-212192 and ENSMUSG00000010358 and 70110, nuclei, protein binding, this GO :0005515 and protein binding and molecular function this GO :0005634 and nucleus and cellular component this GO :0005829 and cytosol and cellular component this GO :0019221 and cytokine-mediated signaling pathway and biological process this GO :0060337 and type I interferon signaling pathway and biological process, this GO :0005515 : protein binding, this GO :0005515 : protein binding, interferon-induced protein 35 |
| Identity: |
5399 |
| Gene: |
IFI35 |
More about : IFI35 |
| Long gene name: |
interferon induced protein 35 |
| Synonyms gene name: |
interferon-induced protein 35 |
| Locus: |
17q21, 31 |
| Discovery year: |
1996-04-12 |
| GenBank acession: |
BC001356 |
| Entrez gene record: |
3430 |
| RefSeq identity: |
NM_005533 |
| Havana BLAST/BLAT: |
OTTHUMG00000167720 |