| Type: |
Conjugated Primary Antibody |
| Conjugated with: |
594, ALEXA FLUOR® |
| Host organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Protein/Peptide: |
IDH1 |
| Specificity: |
This antibody reacts specifically with IDH1 |
| Modification: |
No modification has been applied to this antibody |
| Modification site: |
None |
| Clonality: |
Polyclonal Antibody |
| Clone: |
Polyclonal Antibodies |
| Concentration: |
1ug per 1ul |
| Antigen Source: |
KLH conjugated synthetic peptide derived from human IDH1/Isocitrate dehydrogenase |
| Gene ID: |
3417 |
| Applications: |
IF(IHC-P) |
| Applications with corresponding dilutions: |
IF(IHC-P)(1:50-200) |
| Cross reactive species: |
Mouse (Mus musculus), Rat (Rattus norvegicus), Human (Homo sapiens) |
| Cross Reactive Species details: |
However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species |
| Background information: |
Ca2+ can bind to IDHC as a complex with isocitrate, During this process, IDHC catalyzes the third step of the citric acid cycle, IDHC is expressed in a wide range of species and also in organisms that lack a complete citric acid cycle, The IDHC enzyme is inactivated by phosphorylation at Ser-113 and contains a clasp-like domain wherein both polypeptide chains in the dimer interlock, The first step involves the oxidation of isocitrate to the intermediate oxalosuccinate, acting as a competitive inhibitor of Mg2+, either NADH or NADPH is produced along with CO2, forming ?ketoglutarate and CO2 in a two step reaction, which involves the oxidative decarboxylation of isocitrate, while the second step involves the production of ?ketoglutarate, The Isocitrate dehydrogenase cytoplasmic enzyme is a homodimer of 416 residues that belongs to the isocitrate and isopropylmalate dehydrogenases family |
| Purification method: |
Purified by Protein A |
| Storage: |
50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA |
| Excitation emission: |
590nm/617nm |
| Also known as: |
IDH1 Polyclonal Antibody |
| Other name: |
Anti-IDH1 Polyclonal |
| Advisory: |
For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody |
| Properties: |
For facs or microscopy Alexa 1 conjugate |
| Conjugation: |
Alexa Fluor |
| Group: |
Polyclonals and antibodies |
| About: |
The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera, immunohistochemistry on frozen slices or parrafin fixed tissues, Polyclonals can be used for Western blot |
| Gene target: |
IDH1 |
| Short name: |
Anti-IDH1 (Polyclonal) Fluor 594 |
| Technique: |
Pabs are a collection of immunoglobulin , Polyclonal antibodies , each identifying a different , whereas , (pAbs) are mostly rabbit or goat , B cells, Polyclonal, antibodies , antigen, come from a single N cell lineage, epitope, molecules that react against a specific , monoclonal antibodies , that are secreted by different |
| Label: |
ALEXA |
| Alternative name: |
ALEXA Fluor 594, antibody to-IDH1 (polyclonal) |
| Alternative technique: |
polyclonals |
| Identity: |
5382 |
| Gene: |
IDH1 |
More about : IDH1 |
| Long gene name: |
cytosolic , isocitrate dehydrogenase (NADP(+)) 1 |
| Synonyms gene name: |
isocitrate dehydrogenase 1 (NADP+), soluble isocitrate dehydrogenase 1 (NADP+) |
| Locus: |
2q34 |
| Discovery year: |
2001-06-22 |
| Entrez gene record: |
3417 |
| RefSeq identity: |
NM_005896 |
| Havana BLAST/BLAT: |
OTTHUMG00000132943 |
| Locus Specific Databases: |
LRG_610 |