| Type: |
Conjugated Primary Antibody |
| Conjugated with: |
594, ALEXA FLUOR® |
| Host organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Protein/Peptide: |
SASS6 |
| Specificity: |
This antibody reacts specifically with SASS6 |
| Modification: |
No modification has been applied to this antibody |
| Modification site: |
None |
| Clonality: |
Polyclonal Antibody |
| Clone: |
Polyclonal Antibodies |
| Concentration: |
1ug per 1ul |
| Antigen Source: |
KLH conjugated synthetic peptide derived from human SASS6 |
| Gene ID: |
163786 |
| Applications: |
IF(IHC-P) |
| Applications with corresponding dilutions: |
IF(IHC-P)(1:50-200) |
| Cross reactive species: |
Mouse (Mus musculus), Rat (Rattus norvegicus), Human (Homo sapiens) |
| Cross Reactive Species details: |
However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species |
| Background information: |
CPAP and other centriole related proteins are required at different stages of procentriole formation and were associated with different centriolar structures, HsSAS-6) is a 657 amino acid protein encoded by the human gene SAS6, Increased SAS-6 levels promoted the formation of multiple procentrioles forming next to a single centriole, LK4, SAS-6, SAS-6 associates only transiently with nascent procentrioles, SAS-6 is a component of the centrosome that contains one PISA (present in SAS-6) domain, SAS-6 is necessary for procentriole formation in human cell lines and is localized asymmetrically next to the centriole at the onset of procentriole formation, SAS-6 levels oscillate during the cell cycle, The anaphase-promoting complex targets SAS-6 for degradation by the 26S Proteasome, and a KEN box in the C-terminus of SAS-6 is necessary for its degradation, and it accumulates again at the end of the following G1 phase, it is degraded in mitosis starting at anaphase, whereas CEP135 and CPAP form a core structure within the proximal lumen of both parental and nascent centrioles, SAS-6 (spindle assembly abnormal protein 6 homolog |
| Purification method: |
Purified by Protein A |
| Storage: |
50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA |
| Excitation emission: |
590nm/617nm |
| Synonyms: |
DKFZp761A078 SAS-6 FLJ22097 SAS6 |
| Also known as: |
SASS6 Polyclonal Antibody |
| Other name: |
Anti-SASS6 Polyclonal |
| Advisory: |
For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody |
| Properties: |
For facs or microscopy Alexa 1 conjugate |
| Conjugation: |
Alexa Fluor |
| Group: |
Polyclonals and antibodies |
| About: |
The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera, immunohistochemistry on frozen slices or parrafin fixed tissues, Polyclonals can be used for Western blot |
| Gene target: |
SASS6 |
| Short name: |
Anti-SASS6 (Polyclonal) Fluor 594 |
| Technique: |
Pabs are a collection of immunoglobulin , Polyclonal antibodies , each identifying a different , whereas , (pAbs) are mostly rabbit or goat , B cells, Polyclonal, antibodies , antigen, come from a single N cell lineage, epitope, molecules that react against a specific , monoclonal antibodies , that are secreted by different |
| Label: |
ALEXA |
| Alternative name: |
ALEXA Fluor 594, antibody to-SASS6 (polyclonal) |
| Alternative technique: |
polyclonals |
| Identity: |
25403 |
| Gene: |
SASS6 |
More about : SASS6 |
| Long gene name: |
SAS-6 centriolar assembly protein |
| Synonyms gene name: |
elegans) , spindle assembly 6 homolog (C |
| Locus: |
1p21, 2 |
| Discovery year: |
2005-10-13 |
| GenBank acession: |
AL834265 |
| Entrez gene record: |
163786 |
| Pubmed identfication: |
15665853 14654843 |
| RefSeq identity: |
NM_194292 |
| Havana BLAST/BLAT: |
OTTHUMG00000010754 |