| Type: |
Conjugated Primary Antibody |
| Conjugated with: |
594, ALEXA FLUOR® |
| Host organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Protein/Peptide: |
p53 FL-393 |
| Specificity: |
This antibody reacts specifically with p53 (FL-393) |
| Modification: |
No modification has been applied to this antibody |
| Modification site: |
None |
| Clonality: |
Polyclonal Antibody |
| Clone: |
Polyclonal Antibodies |
| Concentration: |
1ug per 1ul |
| Antigen Source: |
KLH conjugated synthetic peptide derived from human p53 (FL-393) |
| Gene ID: |
7157 |
| Applications: |
IF(IHC-P) |
| Applications with corresponding dilutions: |
IF(IHC-P)(1:50-200) |
| Cross reactive species: |
Mouse (Mus musculus), Rat (Rattus norvegicus), Human (Homo sapiens) |
| Cross Reactive Species details: |
However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species |
| Background information: |
MDM2, MDM2 is an E3 ubiquitin ligase that is upregulated in the presence of active p53, Mutations in the DNA-binding domain (DBD) of p53, a DNA-binding, amino acids 110-286, and cell cycle control mechanisms, can compromise energetically-favorable association with cis elements and are implicated in several human cancers, cell differentiation, including phosphorylation and acetylation, oligomerization domain- and transcription activation domain-containing tumor suppressor, p53 fluctuates between latent and active DNA-binding conformations and is differentially activated through posttranslational modifications, p53 localizes to the nucleus, thereby influencing programmed cell death, upregulates growth arrest and apoptosis-related genes in response to stress signals, where it poly-ubiquitinates p53 for proteasome targeting, yet can be chaperoned to the cytoplasm by the negative regulator, p53 |
| Purification method: |
Purified by Protein A |
| Storage: |
50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA |
| Excitation emission: |
590nm/617nm |
| Synonyms: |
Phosphoprotein p53, TP53, TRP53, Transformation related protein 53, Tumor protein p53, Tumour Protein p53, p53, p53 Cellular Tumor Antigen, p53 Tumor Suppressor, LFS1 |
| Also known as: |
p53 (FL-393) Polyclonal Antibody |
| Other name: |
Anti-p53 (FL-393) Polyclonal |
| Advisory: |
For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody |
| Properties: |
For facs or microscopy Alexa 1 conjugate |
| Conjugation: |
Alexa Fluor |
| Group: |
Polyclonals and antibodies |
| About: |
The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera, immunohistochemistry on frozen slices or parrafin fixed tissues, Polyclonals can be used for Western blot |
| Gene target: |
p53 (FL-393) |
| Short name: |
Anti-p53 (FL-393) (Polyclonal) Fluor 594 |
| Technique: |
Pabs are a collection of immunoglobulin , Polyclonal antibodies , each identifying a different , whereas , (pAbs) are mostly rabbit or goat , B cells, Polyclonal, antibodies , antigen, come from a single N cell lineage, epitope, molecules that react against a specific , monoclonal antibodies , that are secreted by different |
| Label: |
ALEXA |
| Alternative name: |
ALEXA Fluor 594, antibody to-p53 (FL-393) (polyclonal) |
| Alternative technique: |
polyclonals |