| Type: |
Conjugated Primary Antibody |
| Conjugated with: |
594, ALEXA FLUOR® |
| Host organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Protein/Peptide: |
UBE2W |
| Specificity: |
This antibody reacts specifically with UBE2W |
| Modification: |
No modification has been applied to this antibody |
| Modification site: |
None |
| Clonality: |
Polyclonal Antibody |
| Clone: |
Polyclonal Antibodies |
| Concentration: |
1ug per 1ul |
| Antigen Source: |
KLH conjugated synthetic peptide derived from human UBE2W |
| Gene ID: |
55284 |
| Applications: |
IF(IHC-P) |
| Applications with corresponding dilutions: |
IF(IHC-P)(1:50-200) |
| Cross reactive species: |
Mouse (Mus musculus), Rat (Rattus norvegicus), Human (Homo sapiens) |
| Cross Reactive Species details: |
However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species |
| Background information: |
As an E2 class enzyme, Functioning as a homodimer, The three classes of enzymes involved in ubiquitination are the ubiquitin-activating enzymes (E1s), There are two isoforms of UBE2W that are produced as a result of alternative splicing events, UBE2W (Ubiquitin-conjugating enzyme E2 W), UBE2W is widely expressed, Ubiquitination is an important mechanism through which three classes of enzymes act in concert to target short-lived or abnormal proteins for destruction, also known as Ubiquitin carrier protein W, highly conserved protein found in all eukaryotic cells either free or covalently attached to cellular proteins, is a 151 amino acid that catalyzes the conjugation of ubiquitin to proteins that are meant for lysosomal degradation, the ubiquitin-conjugating enzymes (E2s) and the ubiquitin-protein ligases (E3s), with highest levels in testis, Ubiquitin is an abundant |
| Purification method: |
Purified by Protein A |
| Storage: |
50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA |
| Excitation emission: |
590nm/617nm |
| Synonyms: |
FLJ11011 UBC-16 |
| Also known as: |
UBE2W Polyclonal Antibody |
| Other name: |
Anti-UBE2W Polyclonal |
| Advisory: |
For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody |
| Properties: |
For facs or microscopy Alexa 1 conjugate |
| Conjugation: |
Alexa Fluor |
| Group: |
Polyclonals and antibodies |
| About: |
The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera, immunohistochemistry on frozen slices or parrafin fixed tissues, Polyclonals can be used for Western blot |
| Gene target: |
UBE2W |
| Short name: |
Anti-UBE2W (Polyclonal) Fluor 594 |
| Technique: |
Pabs are a collection of immunoglobulin , Polyclonal antibodies , each identifying a different , whereas , (pAbs) are mostly rabbit or goat , B cells, Polyclonal, antibodies , antigen, come from a single N cell lineage, epitope, molecules that react against a specific , monoclonal antibodies , that are secreted by different |
| Label: |
ALEXA |
| Alternative name: |
ALEXA Fluor 594, antibody to-UBE2W (polyclonal) |
| Alternative technique: |
polyclonals |
| Identity: |
25616 |
| Gene: |
UBE2W |
More about : UBE2W |
| Long gene name: |
ubiquitin conjugating enzyme E2 W |
| Synonyms gene name: |
ubiquitin-conjugating enzyme E2W (putative) ubiquitin conjugating enzyme E2 W (putative) |
| Synonyms name: |
E2 ubiquitin-conjugating enzyme W N-terminal E2 ubiquitin-conjugating enzyme N-terminus-conjugating E2 Ubiquitin carrier protein W Ubiquitin-conjugating enzyme 16 Ubiquitin-protein ligase W |
| Locus: |
8q21, 11 |
| Discovery year: |
2005-10-06 |
| GenBank acession: |
AK001873 |
| Entrez gene record: |
55284 |
| Pubmed identfication: |
12477932 |
| RefSeq identity: |
NM_001001481 |
| Classification: |
Ubiquitin conjugating enzymes E2 |
| Havana BLAST/BLAT: |
OTTHUMG00000164517 |