| Type: |
Conjugated Primary Antibody |
| Conjugated with: |
594, ALEXA FLUOR® |
| Host organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Protein/Peptide: |
UBE2D2 |
| Specificity: |
This antibody reacts specifically with UBE2D2 |
| Modification: |
No modification has been applied to this antibody |
| Modification site: |
None |
| Clonality: |
Polyclonal Antibody |
| Clone: |
Polyclonal Antibodies |
| Concentration: |
1ug per 1ul |
| Antigen Source: |
KLH conjugated synthetic peptide derived from human UBE2D2 |
| Gene ID: |
7322 |
| Applications: |
IF(IHC-P) |
| Applications with corresponding dilutions: |
IF(IHC-P)(1:50-200) |
| Cross reactive species: |
Mouse (Mus musculus), Rat (Rattus norvegicus), Human (Homo sapiens) |
| Cross Reactive Species details: |
However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species |
| Background information: |
E1 first transfers a ubiquitin residue to the E2 component (a UBE2D protein), Specifically, The primary function of ubiquitin in mammalian systems is to clear abnormal, UBE2D proteins, UBE2D2 (ubiquitin-conjugating enzyme E2D2 or UBC5B) and UBE2D3 (ubiquitin-conjugating enzyme E2D3 or UBC5C), and improperly folded proteins by targeting them for proteosome degradation, and the UBE2D protein then associates with an E3 ubiquitin-protein ligase, are E2 ubiquitin-conjugating enzymes that catalyze the ubiquitination of I&, foreign, highly conserved protein found in all eukaryotic cells either free or covalently attached to cellular proteins, in a phosphorylation and SCFB-TRCP-dependent manner, including UBE2D1 (ubiquitin-conjugating enzyme E2D1 or UBC5A), which immediately transfers that residue to a protein that is targeted for degradation, #229, #730, B&, Ubiquitin is an abundant |
| Purification method: |
Purified by Protein A |
| Storage: |
50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA |
| Excitation emission: |
590nm/617nm |
| Synonyms: |
UbcH5B UBC4 |
| Also known as: |
UBE2D2 Polyclonal Antibody |
| Other name: |
Anti-UBE2D2 Polyclonal |
| Advisory: |
For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody |
| Properties: |
For facs or microscopy Alexa 1 conjugate |
| Conjugation: |
Alexa Fluor |
| Group: |
Polyclonals and antibodies |
| About: |
The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera, immunohistochemistry on frozen slices or parrafin fixed tissues, Polyclonals can be used for Western blot |
| Gene target: |
UBE2D2 |
| Short name: |
Anti-UBE2D2 (Polyclonal) Fluor 594 |
| Technique: |
Pabs are a collection of immunoglobulin , Polyclonal antibodies , each identifying a different , whereas , (pAbs) are mostly rabbit or goat , B cells, Polyclonal, antibodies , antigen, come from a single N cell lineage, epitope, molecules that react against a specific , monoclonal antibodies , that are secreted by different |
| Label: |
ALEXA |
| Alternative name: |
ALEXA Fluor 594, antibody to-UBE2D2 (polyclonal) |
| Alternative technique: |
polyclonals |
| Identity: |
12475 |
| Gene: |
UBE2D2 |
More about : UBE2D2 |
| Long gene name: |
ubiquitin conjugating enzyme E2 D2 |
| Synonyms gene name: |
ubiquitin-conjugating enzyme E2D 2 (homologous to yeast UBC4/5) ubiquitin-conjugating enzyme E2D 2 (UBC4/5 homolog, yeast) ubiquitin-conjugating enzyme E2D 2 |
| Locus: |
5q31, 2 |
| Discovery year: |
1997-03-21 |
| GenBank acession: |
L40146 |
| Entrez gene record: |
7322 |
| Pubmed identfication: |
8530467 |
| RefSeq identity: |
NM_181838 |
| Classification: |
Ubiquitin conjugating enzymes E2 |
| Havana BLAST/BLAT: |
OTTHUMG00000163282 |