| Type: |
Conjugated Primary Antibody |
| Conjugated with: |
594, ALEXA FLUOR® |
| Host organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Protein/Peptide: |
NEDD4 |
| Specificity: |
This antibody reacts specifically with NEDD4 |
| Modification: |
No modification has been applied to this antibody |
| Modification site: |
None |
| Clonality: |
Polyclonal Antibody |
| Clone: |
Polyclonal Antibodies |
| Concentration: |
1ug per 1ul |
| Antigen Source: |
KLH conjugated synthetic peptide derived from human NEDD4 |
| Gene ID: |
4734 |
| Swiss Prot: |
P46934 |
| Applications: |
IF(IHC-P) |
| Applications with corresponding dilutions: |
IF(IHC-P)(1:50-200) |
| Cross reactive species: |
Mouse (Mus musculus), Rat (Rattus norvegicus), Human (Homo sapiens) |
| Cross Reactive Species details: |
However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species |
| Background information: |
According to PubMed:18562292 the direct link between NEDD4 and PTEN regulation through polyubiquitination described in PubMed:17218260 is questionable, Involved in the budding of many viruses, Involved in the pathway leading to the degradation of VEGFR-2/KDFR, Involved in the ubiquitination of ebola virus VP40 protein and this ubiquitination plays a role in facilitating viral budding, Involved in ubiquitination of ERBB4 intracellular domain E4ICD, Monoubiquitinates IGF1R at multiple sites, Part of a signaling complex composed of NEDD4, Promotes ubiquitination of RAPGEF2, RAP2A and TNIK which regulates neuronal dendrite extension and arborization during development, Ubiquitinates FGFR1, Ubiquitinates TNK2 and regulates EGF-induced degradation of EGFR and TNF2, independently of its ubiquitin-ligase activity, leading to receptor internalization and degradation in lysosomes, thus leading to receptor internalization and degradation in lysosomes, E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates |
| Purification method: |
Purified by Protein A |
| Storage: |
50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA |
| Excitation emission: |
590nm/617nm |
| Synonyms: |
KIAA0093 MGC176705 NEDD4-1 RPF1 |
| Also known as: |
NEDD4 Polyclonal Antibody |
| Other name: |
Anti-NEDD4 Polyclonal |
| Advisory: |
For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody |
| Properties: |
For facs or microscopy Alexa 1 conjugate |
| Conjugation: |
Alexa Fluor |
| Group: |
Polyclonals and antibodies |
| About: |
The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera, immunohistochemistry on frozen slices or parrafin fixed tissues, Polyclonals can be used for Western blot |
| Gene target: |
NEDD4 |
| Short name: |
Anti-NEDD4 (Polyclonal) Fluor 594 |
| Technique: |
Pabs are a collection of immunoglobulin , Polyclonal antibodies , each identifying a different , whereas , (pAbs) are mostly rabbit or goat , B cells, Polyclonal, antibodies , antigen, come from a single N cell lineage, epitope, molecules that react against a specific , monoclonal antibodies , that are secreted by different |
| Label: |
ALEXA |
| Alternative name: |
ALEXA Fluor 594, antibody to-NEDD4 (polyclonal) |
| Alternative technique: |
polyclonals |
| Identity: |
7727 |
| Gene: |
NEDD4 |
More about : NEDD4 |
| Long gene name: |
E3 ubiquitin protein ligase , developmentally down-regulated 4, neural precursor cell expressed |
| Synonyms gene name: |
developmentally down-regulated 4 , neural precursor cell expressed |
| Synonyms name: |
receptor-potentiating factor 1 |
| Locus: |
15q21, 3 |
| Discovery year: |
1994-01-21 |
| GenBank acession: |
D42055 |
| Entrez gene record: |
4734 |
| Pubmed identfication: |
9073511 8649367 |
| RefSeq identity: |
NM_198400 |
| Havana BLAST/BLAT: |
OTTHUMG00000132015 |